Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0052 and Shewana3_0053 are separated by 260 nucleotides Shewana3_0053 and gpsA are separated by 4 nucleotides gpsA and Shewana3_0055 are separated by 238 nucleotides
Shewana3_0052: Shewana3_0052 - rhodanese domain-containing protein (RefSeq), at 61,994 to 62,470
_0052
Shewana3_0053: Shewana3_0053 - preprotein translocase subunit SecB (RefSeq), at 62,731 to 63,216
_0053
Shewana3_0054: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase (RefSeq), at 63,221 to 64,237
gpsA
Shewana3_0055: Shewana3_0055 - hypothetical protein (RefSeq), at 64,476 to 65,660
_0055
Position (kb) 63
64
65 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 62.255 kb on + strand, within Shewana3_0052 at 62.332 kb on + strand, within Shewana3_0052 at 62.332 kb on + strand, within Shewana3_0052 at 62.484 kb on + strand at 62.494 kb on + strand at 64.344 kb on - strand at 64.397 kb on + strand at 64.404 kb on - strand at 64.474 kb on - strand at 64.518 kb on + strand at 64.744 kb on + strand, within Shewana3_0055 at 64.765 kb on + strand, within Shewana3_0055 at 64.767 kb on - strand, within Shewana3_0055 at 64.846 kb on + strand, within Shewana3_0055 at 64.925 kb on + strand, within Shewana3_0055 at 64.988 kb on + strand, within Shewana3_0055 at 64.989 kb on + strand, within Shewana3_0055 at 64.989 kb on + strand, within Shewana3_0055 at 65.178 kb on + strand, within Shewana3_0055 at 65.186 kb on - strand, within Shewana3_0055
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 62,255 + Shewana3_0052 0.55 -2.8 62,332 + Shewana3_0052 0.71 -1.9 62,332 + Shewana3_0052 0.71 -0.9 62,484 + +1.0 62,494 + -0.0 64,344 - +0.0 64,397 + -0.9 64,404 - +0.3 64,474 - -1.0 64,518 + -0.1 64,744 + Shewana3_0055 0.23 -0.4 64,765 + Shewana3_0055 0.24 -0.1 64,767 - Shewana3_0055 0.25 -0.3 64,846 + Shewana3_0055 0.31 +0.1 64,925 + Shewana3_0055 0.38 +0.1 64,988 + Shewana3_0055 0.43 +0.1 64,989 + Shewana3_0055 0.43 +0.2 64,989 + Shewana3_0055 0.43 +0.1 65,178 + Shewana3_0055 0.59 -0.2 65,186 - Shewana3_0055 0.60 -0.0
Or see this region's nucleotide sequence