Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0050 and Shewana3_0051 are separated by 10 nucleotides Shewana3_0051 and Shewana3_0052 are separated by 223 nucleotides Shewana3_0052 and Shewana3_0053 are separated by 260 nucleotides
Shewana3_0050: Shewana3_0050 - peptidase M23B (RefSeq), at 59,082 to 60,215
_0050
Shewana3_0051: Shewana3_0051 - phosphoglyceromutase (RefSeq), at 60,226 to 61,770
_0051
Shewana3_0052: Shewana3_0052 - rhodanese domain-containing protein (RefSeq), at 61,994 to 62,470
_0052
Shewana3_0053: Shewana3_0053 - preprotein translocase subunit SecB (RefSeq), at 62,731 to 63,216
_0053
Position (kb) 60
61
62 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 59.301 kb on - strand, within Shewana3_0050 at 59.302 kb on - strand, within Shewana3_0050 at 60.109 kb on + strand at 60.211 kb on - strand at 61.862 kb on + strand at 61.870 kb on - strand at 61.900 kb on - strand at 61.928 kb on + strand at 61.984 kb on + strand at 61.992 kb on - strand at 62.034 kb on - strand at 62.080 kb on - strand, within Shewana3_0052 at 62.165 kb on + strand, within Shewana3_0052 at 62.180 kb on - strand, within Shewana3_0052 at 62.255 kb on + strand, within Shewana3_0052 at 62.332 kb on + strand, within Shewana3_0052 at 62.332 kb on + strand, within Shewana3_0052 at 62.484 kb on + strand at 62.494 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 59,301 - Shewana3_0050 0.19 -2.2 59,302 - Shewana3_0050 0.19 -0.8 60,109 + -2.0 60,211 - -0.6 61,862 + +0.2 61,870 - +0.7 61,900 - -0.1 61,928 + -0.2 61,984 + -0.9 61,992 - -4.1 62,034 - -4.8 62,080 - Shewana3_0052 0.18 -1.7 62,165 + Shewana3_0052 0.36 -4.2 62,180 - Shewana3_0052 0.39 -0.3 62,255 + Shewana3_0052 0.55 -2.8 62,332 + Shewana3_0052 0.71 -1.9 62,332 + Shewana3_0052 0.71 -0.9 62,484 + +1.0 62,494 + -0.0
Or see this region's nucleotide sequence