Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0033 and Shewana3_0034 overlap by 1 nucleotides Shewana3_0034 and def are separated by 15 nucleotides def and Shewana3_0036 are separated by 125 nucleotides
Shewana3_0033: Shewana3_0033 - sun protein (RefSeq), at 40,083 to 41,372
_0033
Shewana3_0034: Shewana3_0034 - methionyl-tRNA formyltransferase (RefSeq), at 41,372 to 42,328
_0034
Shewana3_0035: def - peptide deformylase (RefSeq), at 42,344 to 42,850
def
Shewana3_0036: Shewana3_0036 - peptidoglycan-binding LysM (RefSeq), at 42,976 to 44,100
_0036
Position (kb) 41
42
43 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 40.411 kb on + strand, within Shewana3_0033 at 40.414 kb on - strand, within Shewana3_0033 at 40.419 kb on - strand, within Shewana3_0033 at 40.419 kb on - strand, within Shewana3_0033 at 40.443 kb on + strand, within Shewana3_0033 at 40.451 kb on - strand, within Shewana3_0033 at 40.583 kb on + strand, within Shewana3_0033 at 40.677 kb on + strand, within Shewana3_0033 at 40.685 kb on - strand, within Shewana3_0033 at 40.772 kb on - strand, within Shewana3_0033 at 40.814 kb on - strand, within Shewana3_0033 at 40.875 kb on + strand, within Shewana3_0033 at 40.889 kb on + strand, within Shewana3_0033 at 40.934 kb on + strand, within Shewana3_0033 at 40.958 kb on + strand, within Shewana3_0033 at 40.958 kb on + strand, within Shewana3_0033 at 40.966 kb on - strand, within Shewana3_0033 at 40.978 kb on + strand, within Shewana3_0033 at 41.020 kb on + strand, within Shewana3_0033 at 41.030 kb on + strand, within Shewana3_0033 at 41.094 kb on + strand, within Shewana3_0033 at 41.152 kb on + strand, within Shewana3_0033 at 41.210 kb on + strand, within Shewana3_0033 at 41.218 kb on - strand, within Shewana3_0033 at 41.252 kb on + strand at 41.302 kb on + strand at 41.330 kb on - strand at 41.404 kb on + strand at 42.520 kb on - strand, within def at 42.527 kb on - strand, within def at 42.637 kb on - strand, within def at 42.774 kb on + strand, within def at 43.247 kb on - strand, within Shewana3_0036 at 43.247 kb on - strand, within Shewana3_0036 at 43.247 kb on - strand, within Shewana3_0036 at 43.247 kb on - strand, within Shewana3_0036 at 43.247 kb on - strand, within Shewana3_0036 at 43.292 kb on + strand, within Shewana3_0036 at 43.292 kb on + strand, within Shewana3_0036 at 43.300 kb on - strand, within Shewana3_0036 at 43.300 kb on - strand, within Shewana3_0036 at 43.302 kb on - strand, within Shewana3_0036
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 40,411 + Shewana3_0033 0.25 -1.1 40,414 - Shewana3_0033 0.26 +0.6 40,419 - Shewana3_0033 0.26 -0.1 40,419 - Shewana3_0033 0.26 -2.0 40,443 + Shewana3_0033 0.28 -0.9 40,451 - Shewana3_0033 0.29 -1.4 40,583 + Shewana3_0033 0.39 -1.9 40,677 + Shewana3_0033 0.46 -1.8 40,685 - Shewana3_0033 0.47 -1.4 40,772 - Shewana3_0033 0.53 -1.6 40,814 - Shewana3_0033 0.57 -1.5 40,875 + Shewana3_0033 0.61 -3.3 40,889 + Shewana3_0033 0.62 -2.8 40,934 + Shewana3_0033 0.66 -1.3 40,958 + Shewana3_0033 0.68 -2.5 40,958 + Shewana3_0033 0.68 -1.6 40,966 - Shewana3_0033 0.68 -2.1 40,978 + Shewana3_0033 0.69 -1.7 41,020 + Shewana3_0033 0.73 -1.8 41,030 + Shewana3_0033 0.73 -1.8 41,094 + Shewana3_0033 0.78 -0.9 41,152 + Shewana3_0033 0.83 -2.7 41,210 + Shewana3_0033 0.87 -2.7 41,218 - Shewana3_0033 0.88 -1.2 41,252 + -4.4 41,302 + -2.0 41,330 - -0.8 41,404 + -0.9 42,520 - def Shewana3_0035 0.35 -0.1 42,527 - def Shewana3_0035 0.36 -1.2 42,637 - def Shewana3_0035 0.58 +0.0 42,774 + def Shewana3_0035 0.85 -2.0 43,247 - Shewana3_0036 0.24 -0.1 43,247 - Shewana3_0036 0.24 +0.2 43,247 - Shewana3_0036 0.24 +0.3 43,247 - Shewana3_0036 0.24 +0.2 43,247 - Shewana3_0036 0.24 -0.5 43,292 + Shewana3_0036 0.28 -1.1 43,292 + Shewana3_0036 0.28 -1.1 43,300 - Shewana3_0036 0.29 +0.3 43,300 - Shewana3_0036 0.29 +0.3 43,302 - Shewana3_0036 0.29 -0.9
Or see this region's nucleotide sequence