Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4382 and Shewana3_4383 are separated by 19 nucleotides Shewana3_4383 and Shewana3_4384 overlap by 11 nucleotides Shewana3_4384 and Shewana3_4385 are separated by 11 nucleotides
Shewana3_4382: Shewana3_4382 - hypothetical protein (RefSeq), at 268,001 to 268,531
_4382
Shewana3_4383: Shewana3_4383 - hypothetical protein (RefSeq), at 268,551 to 269,246
_4383
Shewana3_4384: Shewana3_4384 - CzcA family heavy metal efflux protein (RefSeq), at 269,236 to 272,379
_4384
Shewana3_4385: Shewana3_4385 - secretion protein HlyD (RefSeq), at 272,391 to 273,635
_4385
Position (kb) 269
270
271
272
273 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 268.319 kb on - strand, within Shewana3_4382 at 268.336 kb on + strand, within Shewana3_4382 at 268.359 kb on - strand, within Shewana3_4382 at 268.433 kb on - strand, within Shewana3_4382 at 268.567 kb on + strand at 268.595 kb on + strand at 268.595 kb on + strand at 268.595 kb on + strand at 268.595 kb on + strand at 268.595 kb on + strand at 268.595 kb on + strand at 268.603 kb on - strand at 268.613 kb on - strand at 268.689 kb on - strand, within Shewana3_4383 at 268.790 kb on + strand, within Shewana3_4383 at 268.798 kb on - strand, within Shewana3_4383 at 269.085 kb on - strand, within Shewana3_4383 at 269.226 kb on - strand at 269.391 kb on + strand at 269.399 kb on - strand at 269.463 kb on - strand at 269.477 kb on + strand at 269.550 kb on + strand at 269.740 kb on - strand, within Shewana3_4384 at 269.823 kb on - strand, within Shewana3_4384 at 270.042 kb on + strand, within Shewana3_4384 at 270.127 kb on - strand, within Shewana3_4384 at 270.158 kb on + strand, within Shewana3_4384 at 270.404 kb on - strand, within Shewana3_4384 at 270.712 kb on + strand, within Shewana3_4384 at 270.804 kb on - strand, within Shewana3_4384 at 270.804 kb on - strand, within Shewana3_4384 at 270.920 kb on - strand, within Shewana3_4384 at 271.213 kb on + strand, within Shewana3_4384 at 271.307 kb on - strand, within Shewana3_4384 at 271.326 kb on - strand, within Shewana3_4384 at 271.348 kb on - strand, within Shewana3_4384 at 271.502 kb on + strand, within Shewana3_4384 at 271.672 kb on + strand, within Shewana3_4384 at 271.752 kb on + strand, within Shewana3_4384 at 271.839 kb on + strand, within Shewana3_4384 at 271.850 kb on + strand, within Shewana3_4384 at 271.850 kb on + strand, within Shewana3_4384 at 271.858 kb on - strand, within Shewana3_4384 at 272.019 kb on + strand, within Shewana3_4384 at 272.104 kb on - strand at 272.134 kb on + strand at 272.142 kb on - strand at 272.142 kb on - strand at 272.149 kb on + strand at 272.183 kb on + strand at 272.191 kb on - strand at 272.206 kb on - strand at 272.251 kb on + strand at 272.496 kb on - strand at 272.538 kb on + strand, within Shewana3_4385 at 272.553 kb on - strand, within Shewana3_4385 at 272.633 kb on + strand, within Shewana3_4385 at 272.872 kb on + strand, within Shewana3_4385 at 273.031 kb on - strand, within Shewana3_4385
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 268,319 - Shewana3_4382 0.60 +0.1 268,336 + Shewana3_4382 0.63 -1.1 268,359 - Shewana3_4382 0.67 -0.1 268,433 - Shewana3_4382 0.81 -0.2 268,567 + +0.3 268,595 + +0.9 268,595 + -0.8 268,595 + +0.1 268,595 + -0.9 268,595 + -0.8 268,595 + +1.9 268,603 - -0.9 268,613 - -0.0 268,689 - Shewana3_4383 0.20 +0.4 268,790 + Shewana3_4383 0.34 -0.9 268,798 - Shewana3_4383 0.35 -0.2 269,085 - Shewana3_4383 0.77 -1.2 269,226 - +0.0 269,391 + +0.3 269,399 - -0.1 269,463 - +0.2 269,477 + -1.8 269,550 + -1.4 269,740 - Shewana3_4384 0.16 -0.4 269,823 - Shewana3_4384 0.19 -0.1 270,042 + Shewana3_4384 0.26 -0.1 270,127 - Shewana3_4384 0.28 +0.9 270,158 + Shewana3_4384 0.29 -0.8 270,404 - Shewana3_4384 0.37 -0.6 270,712 + Shewana3_4384 0.47 +0.7 270,804 - Shewana3_4384 0.50 +0.6 270,804 - Shewana3_4384 0.50 +0.3 270,920 - Shewana3_4384 0.54 +0.3 271,213 + Shewana3_4384 0.63 -1.0 271,307 - Shewana3_4384 0.66 +0.9 271,326 - Shewana3_4384 0.66 +0.8 271,348 - Shewana3_4384 0.67 +0.7 271,502 + Shewana3_4384 0.72 -0.4 271,672 + Shewana3_4384 0.77 +0.6 271,752 + Shewana3_4384 0.80 -0.4 271,839 + Shewana3_4384 0.83 -0.4 271,850 + Shewana3_4384 0.83 -0.4 271,850 + Shewana3_4384 0.83 -0.1 271,858 - Shewana3_4384 0.83 +1.1 272,019 + Shewana3_4384 0.89 +0.0 272,104 - +0.2 272,134 + -0.3 272,142 - +0.1 272,142 - -0.5 272,149 + +0.3 272,183 + +0.3 272,191 - -0.7 272,206 - +0.2 272,251 + +0.3 272,496 - +0.4 272,538 + Shewana3_4385 0.12 -0.2 272,553 - Shewana3_4385 0.13 +0.3 272,633 + Shewana3_4385 0.19 -3.0 272,872 + Shewana3_4385 0.39 -0.5 273,031 - Shewana3_4385 0.51 +0.0
Or see this region's nucleotide sequence