Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4375 and Shewana3_4376 are separated by 76 nucleotides Shewana3_4376 and Shewana3_4377 overlap by 23 nucleotides Shewana3_4377 and Shewana3_4378 are separated by 78 nucleotides Shewana3_4378 and Shewana3_4379 are separated by 8 nucleotides
Shewana3_4375: Shewana3_4375 - hypothetical protein (RefSeq), at 263,170 to 263,847
_4375
Shewana3_4376: Shewana3_4376 - hypothetical protein (RefSeq), at 263,924 to 264,508
_4376
Shewana3_4377: Shewana3_4377 - phage integrase family protein (RefSeq), at 264,486 to 265,601
_4377
Shewana3_4378: Shewana3_4378 - hypothetical protein (RefSeq), at 265,680 to 266,051
_4378
Shewana3_4379: Shewana3_4379 - hypothetical protein (RefSeq), at 266,060 to 266,965
_4379
Position (kb) 264
265
266 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 263.702 kb on + strand, within Shewana3_4375 at 264.032 kb on - strand, within Shewana3_4376 at 264.057 kb on + strand, within Shewana3_4376 at 264.480 kb on + strand at 264.522 kb on - strand at 264.524 kb on + strand at 264.524 kb on + strand at 264.524 kb on + strand at 264.541 kb on + strand at 264.583 kb on + strand at 264.674 kb on + strand, within Shewana3_4377 at 264.920 kb on + strand, within Shewana3_4377 at 264.920 kb on + strand, within Shewana3_4377 at 264.920 kb on + strand, within Shewana3_4377 at 264.965 kb on + strand, within Shewana3_4377 at 264.995 kb on + strand, within Shewana3_4377 at 265.028 kb on - strand, within Shewana3_4377 at 265.276 kb on + strand, within Shewana3_4377 at 265.276 kb on + strand, within Shewana3_4377 at 265.338 kb on + strand, within Shewana3_4377 at 265.346 kb on - strand, within Shewana3_4377 at 265.550 kb on + strand at 265.558 kb on - strand at 266.071 kb on - strand at 266.125 kb on + strand at 266.139 kb on - strand at 266.215 kb on + strand, within Shewana3_4379 at 266.223 kb on - strand, within Shewana3_4379 at 266.301 kb on - strand, within Shewana3_4379 at 266.435 kb on - strand, within Shewana3_4379 at 266.474 kb on - strand, within Shewana3_4379 at 266.478 kb on + strand, within Shewana3_4379 at 266.528 kb on + strand, within Shewana3_4379 at 266.533 kb on + strand, within Shewana3_4379 at 266.548 kb on - strand, within Shewana3_4379
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 263,702 + Shewana3_4375 0.78 +0.1 264,032 - Shewana3_4376 0.18 -0.1 264,057 + Shewana3_4376 0.23 +0.5 264,480 + +0.6 264,522 - -2.2 264,524 + -2.4 264,524 + +0.2 264,524 + -1.6 264,541 + -0.8 264,583 + +0.9 264,674 + Shewana3_4377 0.17 +0.0 264,920 + Shewana3_4377 0.39 +1.3 264,920 + Shewana3_4377 0.39 -0.7 264,920 + Shewana3_4377 0.39 +0.0 264,965 + Shewana3_4377 0.43 +0.3 264,995 + Shewana3_4377 0.46 +0.8 265,028 - Shewana3_4377 0.49 -1.2 265,276 + Shewana3_4377 0.71 +0.4 265,276 + Shewana3_4377 0.71 +1.1 265,338 + Shewana3_4377 0.76 -0.7 265,346 - Shewana3_4377 0.77 -1.0 265,550 + -1.6 265,558 - -2.5 266,071 - +1.9 266,125 + -2.8 266,139 - -1.4 266,215 + Shewana3_4379 0.17 +0.1 266,223 - Shewana3_4379 0.18 +0.9 266,301 - Shewana3_4379 0.27 -0.3 266,435 - Shewana3_4379 0.41 -0.9 266,474 - Shewana3_4379 0.46 -0.4 266,478 + Shewana3_4379 0.46 -2.2 266,528 + Shewana3_4379 0.52 -0.5 266,533 + Shewana3_4379 0.52 -2.3 266,548 - Shewana3_4379 0.54 +0.3
Or see this region's nucleotide sequence