Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4372 and Shewana3_4373 are separated by 91 nucleotides Shewana3_4373 and Shewana3_4374 overlap by 1 nucleotides Shewana3_4374 and Shewana3_4375 are separated by 105 nucleotides Shewana3_4375 and Shewana3_4376 are separated by 76 nucleotides
Shewana3_4372: Shewana3_4372 - hypothetical protein (RefSeq), at 260,617 to 262,353
_4372
Shewana3_4373: Shewana3_4373 - hypothetical protein (RefSeq), at 262,445 to 262,816
_4373
Shewana3_4374: Shewana3_4374 - hypothetical protein (RefSeq), at 262,816 to 263,064
_4374
Shewana3_4375: Shewana3_4375 - hypothetical protein (RefSeq), at 263,170 to 263,847
_4375
Shewana3_4376: Shewana3_4376 - hypothetical protein (RefSeq), at 263,924 to 264,508
_4376
Position (kb) 262
263
264 Strain fitness (log2 ratio) -2
-1
0
1
2 at 261.842 kb on + strand, within Shewana3_4372 at 261.934 kb on + strand, within Shewana3_4372 at 261.947 kb on + strand, within Shewana3_4372 at 262.040 kb on + strand, within Shewana3_4372 at 262.041 kb on + strand, within Shewana3_4372 at 262.042 kb on + strand, within Shewana3_4372 at 262.048 kb on - strand, within Shewana3_4372 at 262.117 kb on - strand, within Shewana3_4372 at 262.128 kb on - strand, within Shewana3_4372 at 262.151 kb on - strand, within Shewana3_4372 at 262.268 kb on - strand at 262.394 kb on + strand at 262.533 kb on - strand, within Shewana3_4373 at 262.556 kb on - strand, within Shewana3_4373 at 262.744 kb on - strand, within Shewana3_4373 at 262.862 kb on - strand, within Shewana3_4374 at 262.873 kb on + strand, within Shewana3_4374 at 262.953 kb on + strand, within Shewana3_4374 at 263.024 kb on + strand, within Shewana3_4374 at 263.241 kb on + strand, within Shewana3_4375 at 263.341 kb on - strand, within Shewana3_4375 at 263.416 kb on - strand, within Shewana3_4375 at 263.702 kb on + strand, within Shewana3_4375 at 264.032 kb on - strand, within Shewana3_4376 at 264.057 kb on + strand, within Shewana3_4376
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 261,842 + Shewana3_4372 0.71 -1.2 261,934 + Shewana3_4372 0.76 +0.3 261,947 + Shewana3_4372 0.77 +0.0 262,040 + Shewana3_4372 0.82 +1.0 262,041 + Shewana3_4372 0.82 +1.8 262,042 + Shewana3_4372 0.82 +0.1 262,048 - Shewana3_4372 0.82 +0.0 262,117 - Shewana3_4372 0.86 +0.3 262,128 - Shewana3_4372 0.87 -1.5 262,151 - Shewana3_4372 0.88 -0.2 262,268 - +0.3 262,394 + +0.1 262,533 - Shewana3_4373 0.24 -0.1 262,556 - Shewana3_4373 0.30 +1.2 262,744 - Shewana3_4373 0.80 -1.5 262,862 - Shewana3_4374 0.18 -2.0 262,873 + Shewana3_4374 0.23 -0.5 262,953 + Shewana3_4374 0.55 +1.0 263,024 + Shewana3_4374 0.84 +0.0 263,241 + Shewana3_4375 0.10 -0.7 263,341 - Shewana3_4375 0.25 -0.9 263,416 - Shewana3_4375 0.36 -0.0 263,702 + Shewana3_4375 0.78 +0.1 264,032 - Shewana3_4376 0.18 -0.1 264,057 + Shewana3_4376 0.23 +0.5
Or see this region's nucleotide sequence