Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4332 and Shewana3_4333 are separated by 54 nucleotides Shewana3_4333 and Shewana3_4334 are separated by 31 nucleotides Shewana3_4334 and Shewana3_4335 are separated by 27 nucleotides Shewana3_4335 and Shewana3_4336 are separated by 122 nucleotides
Shewana3_4332: Shewana3_4332 - hypothetical protein (RefSeq), at 222,537 to 222,782
_4332
Shewana3_4333: Shewana3_4333 - methionine-R-sulfoxide reductase (RefSeq), at 222,837 to 223,277
_4333
Shewana3_4334: Shewana3_4334 - glutaredoxin (RefSeq), at 223,309 to 223,545
_4334
Shewana3_4335: Shewana3_4335 - peptide methionine sulfoxide reductase (RefSeq), at 223,573 to 224,061
_4335
Shewana3_4336: Shewana3_4336 - AraC family transcriptional regulator (RefSeq), at 224,184 to 225,119
_4336
Position (kb) 223
224
225 Strain fitness (log2 ratio) -1
0
1 at 222.616 kb on - strand, within Shewana3_4332 at 222.641 kb on + strand, within Shewana3_4332 at 222.641 kb on + strand, within Shewana3_4332 at 222.763 kb on - strand at 222.763 kb on - strand at 222.822 kb on - strand at 222.865 kb on - strand at 222.890 kb on + strand, within Shewana3_4333 at 222.890 kb on + strand, within Shewana3_4333 at 222.898 kb on - strand, within Shewana3_4333 at 222.898 kb on - strand, within Shewana3_4333 at 222.988 kb on + strand, within Shewana3_4333 at 223.010 kb on - strand, within Shewana3_4333 at 223.092 kb on + strand, within Shewana3_4333 at 223.239 kb on - strand at 223.673 kb on + strand, within Shewana3_4335 at 223.681 kb on - strand, within Shewana3_4335 at 223.782 kb on - strand, within Shewana3_4335 at 223.791 kb on + strand, within Shewana3_4335 at 223.997 kb on + strand, within Shewana3_4335 at 224.005 kb on - strand, within Shewana3_4335 at 224.155 kb on + strand at 224.220 kb on - strand at 224.245 kb on - strand at 224.398 kb on + strand, within Shewana3_4336 at 224.403 kb on + strand, within Shewana3_4336 at 224.406 kb on - strand, within Shewana3_4336 at 224.416 kb on - strand, within Shewana3_4336 at 224.600 kb on + strand, within Shewana3_4336 at 224.600 kb on + strand, within Shewana3_4336 at 224.606 kb on - strand, within Shewana3_4336 at 224.758 kb on - strand, within Shewana3_4336 at 224.859 kb on - strand, within Shewana3_4336 at 224.874 kb on + strand, within Shewana3_4336 at 224.923 kb on - strand, within Shewana3_4336
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 222,616 - Shewana3_4332 0.32 -0.0 222,641 + Shewana3_4332 0.42 -0.2 222,641 + Shewana3_4332 0.42 -0.7 222,763 - +0.4 222,763 - +1.2 222,822 - +1.2 222,865 - -0.0 222,890 + Shewana3_4333 0.12 -1.1 222,890 + Shewana3_4333 0.12 -1.1 222,898 - Shewana3_4333 0.14 +0.3 222,898 - Shewana3_4333 0.14 +0.5 222,988 + Shewana3_4333 0.34 -1.7 223,010 - Shewana3_4333 0.39 +0.0 223,092 + Shewana3_4333 0.58 -0.7 223,239 - +0.4 223,673 + Shewana3_4335 0.20 -0.7 223,681 - Shewana3_4335 0.22 +0.2 223,782 - Shewana3_4335 0.43 +1.1 223,791 + Shewana3_4335 0.45 +0.9 223,997 + Shewana3_4335 0.87 -1.5 224,005 - Shewana3_4335 0.88 +0.5 224,155 + +0.5 224,220 - -0.1 224,245 - +0.1 224,398 + Shewana3_4336 0.23 -0.1 224,403 + Shewana3_4336 0.23 +0.8 224,406 - Shewana3_4336 0.24 -0.2 224,416 - Shewana3_4336 0.25 -0.1 224,600 + Shewana3_4336 0.44 +0.2 224,600 + Shewana3_4336 0.44 +0.4 224,606 - Shewana3_4336 0.45 -1.2 224,758 - Shewana3_4336 0.61 -0.1 224,859 - Shewana3_4336 0.72 -1.4 224,874 + Shewana3_4336 0.74 +0.1 224,923 - Shewana3_4336 0.79 -0.2
Or see this region's nucleotide sequence