Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4330 and Shewana3_4331 are separated by 219 nucleotides Shewana3_4331 and Shewana3_4332 are separated by 205 nucleotides Shewana3_4332 and Shewana3_4333 are separated by 54 nucleotides Shewana3_4333 and Shewana3_4334 are separated by 31 nucleotides
Shewana3_4330: Shewana3_4330 - diguanylate cyclase with GAF sensor (RefSeq), at 220,472 to 221,449
_4330
Shewana3_4331: Shewana3_4331 - methionine sulfoxide reductase A (RefSeq), at 221,669 to 222,331
_4331
Shewana3_4332: Shewana3_4332 - hypothetical protein (RefSeq), at 222,537 to 222,782
_4332
Shewana3_4333: Shewana3_4333 - methionine-R-sulfoxide reductase (RefSeq), at 222,837 to 223,277
_4333
Shewana3_4334: Shewana3_4334 - glutaredoxin (RefSeq), at 223,309 to 223,545
_4334
Position (kb) 221
222
223 Strain fitness (log2 ratio) -2
-1
0
1 at 220.701 kb on + strand, within Shewana3_4330 at 220.701 kb on + strand, within Shewana3_4330 at 220.709 kb on - strand, within Shewana3_4330 at 220.733 kb on + strand, within Shewana3_4330 at 221.143 kb on + strand, within Shewana3_4330 at 221.333 kb on - strand, within Shewana3_4330 at 221.471 kb on + strand at 221.479 kb on - strand at 221.589 kb on + strand at 221.589 kb on + strand at 221.597 kb on - strand at 221.710 kb on + strand at 221.720 kb on + strand at 221.728 kb on - strand at 221.728 kb on - strand at 221.728 kb on - strand at 222.011 kb on + strand, within Shewana3_4331 at 222.029 kb on - strand, within Shewana3_4331 at 222.081 kb on + strand, within Shewana3_4331 at 222.098 kb on - strand, within Shewana3_4331 at 222.275 kb on - strand at 222.298 kb on - strand at 222.364 kb on + strand at 222.616 kb on - strand, within Shewana3_4332 at 222.641 kb on + strand, within Shewana3_4332 at 222.641 kb on + strand, within Shewana3_4332 at 222.763 kb on - strand at 222.763 kb on - strand at 222.822 kb on - strand at 222.865 kb on - strand at 222.890 kb on + strand, within Shewana3_4333 at 222.890 kb on + strand, within Shewana3_4333 at 222.898 kb on - strand, within Shewana3_4333 at 222.898 kb on - strand, within Shewana3_4333 at 222.988 kb on + strand, within Shewana3_4333 at 223.010 kb on - strand, within Shewana3_4333 at 223.092 kb on + strand, within Shewana3_4333 at 223.239 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 220,701 + Shewana3_4330 0.23 +0.1 220,701 + Shewana3_4330 0.23 -0.7 220,709 - Shewana3_4330 0.24 -1.6 220,733 + Shewana3_4330 0.27 +1.6 221,143 + Shewana3_4330 0.69 +0.6 221,333 - Shewana3_4330 0.88 +0.2 221,471 + -0.1 221,479 - -0.6 221,589 + -0.3 221,589 + -2.7 221,597 - +1.2 221,710 + +0.9 221,720 + +1.3 221,728 - +0.4 221,728 - +0.2 221,728 - -0.6 222,011 + Shewana3_4331 0.52 -2.2 222,029 - Shewana3_4331 0.54 -1.0 222,081 + Shewana3_4331 0.62 +0.2 222,098 - Shewana3_4331 0.65 -0.6 222,275 - +0.8 222,298 - -0.7 222,364 + -0.7 222,616 - Shewana3_4332 0.32 -0.0 222,641 + Shewana3_4332 0.42 -0.2 222,641 + Shewana3_4332 0.42 -0.7 222,763 - +0.4 222,763 - +1.2 222,822 - +1.2 222,865 - -0.0 222,890 + Shewana3_4333 0.12 -1.1 222,890 + Shewana3_4333 0.12 -1.1 222,898 - Shewana3_4333 0.14 +0.3 222,898 - Shewana3_4333 0.14 +0.5 222,988 + Shewana3_4333 0.34 -1.7 223,010 - Shewana3_4333 0.39 +0.0 223,092 + Shewana3_4333 0.58 -0.7 223,239 - +0.4
Or see this region's nucleotide sequence