Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4292 and Shewana3_4293 are separated by 456 nucleotides Shewana3_4293 and Shewana3_4294 are separated by 465 nucleotides Shewana3_4294 and Shewana3_4295 are separated by 169 nucleotides
Shewana3_4292: Shewana3_4292 - hypothetical protein (RefSeq), at 174,969 to 175,514
_4292
Shewana3_4293: Shewana3_4293 - outer membrane porin (RefSeq), at 175,971 to 177,167
_4293
Shewana3_4294: Shewana3_4294 - hypothetical protein (RefSeq), at 177,633 to 177,914
_4294
Shewana3_4295: Shewana3_4295 - sensor kinase CusS (RefSeq), at 178,084 to 179,559
_4295
Position (kb) 175
176
177
178 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 174.985 kb on - strand at 175.005 kb on - strand at 175.025 kb on - strand, within Shewana3_4292 at 175.025 kb on - strand, within Shewana3_4292 at 175.027 kb on + strand, within Shewana3_4292 at 175.115 kb on + strand, within Shewana3_4292 at 175.161 kb on - strand, within Shewana3_4292 at 175.168 kb on + strand at 175.212 kb on + strand, within Shewana3_4292 at 175.230 kb on - strand, within Shewana3_4292 at 175.252 kb on + strand, within Shewana3_4292 at 175.252 kb on + strand, within Shewana3_4292 at 175.252 kb on + strand, within Shewana3_4292 at 175.252 kb on + strand, within Shewana3_4292 at 175.260 kb on - strand, within Shewana3_4292 at 175.306 kb on + strand, within Shewana3_4292 at 175.388 kb on + strand, within Shewana3_4292 at 175.396 kb on - strand, within Shewana3_4292 at 175.411 kb on + strand, within Shewana3_4292 at 175.421 kb on - strand, within Shewana3_4292 at 175.466 kb on + strand at 175.515 kb on + strand at 175.523 kb on - strand at 175.528 kb on - strand at 175.608 kb on - strand at 175.637 kb on - strand at 175.683 kb on - strand at 175.685 kb on + strand at 175.691 kb on - strand at 175.697 kb on - strand at 175.710 kb on + strand at 175.718 kb on - strand at 175.993 kb on + strand at 176.001 kb on - strand at 176.003 kb on + strand at 176.011 kb on - strand at 176.036 kb on + strand at 176.059 kb on - strand at 176.072 kb on - strand at 176.177 kb on + strand, within Shewana3_4293 at 176.190 kb on + strand, within Shewana3_4293 at 176.196 kb on + strand, within Shewana3_4293 at 176.197 kb on + strand, within Shewana3_4293 at 176.197 kb on + strand, within Shewana3_4293 at 176.197 kb on + strand, within Shewana3_4293 at 176.197 kb on + strand, within Shewana3_4293 at 176.197 kb on + strand, within Shewana3_4293 at 176.197 kb on + strand, within Shewana3_4293 at 176.198 kb on - strand, within Shewana3_4293 at 176.228 kb on + strand, within Shewana3_4293 at 176.262 kb on - strand, within Shewana3_4293 at 176.267 kb on - strand, within Shewana3_4293 at 176.268 kb on - strand, within Shewana3_4293 at 176.309 kb on + strand, within Shewana3_4293 at 176.515 kb on + strand, within Shewana3_4293 at 176.681 kb on + strand, within Shewana3_4293 at 176.759 kb on + strand, within Shewana3_4293 at 176.874 kb on - strand at 176.875 kb on + strand, within Shewana3_4293 at 176.876 kb on + strand, within Shewana3_4293 at 176.876 kb on + strand, within Shewana3_4293 at 176.888 kb on + strand, within Shewana3_4293 at 176.896 kb on - strand, within Shewana3_4293 at 176.910 kb on - strand, within Shewana3_4293 at 176.943 kb on + strand, within Shewana3_4293 at 176.951 kb on - strand, within Shewana3_4293 at 176.951 kb on - strand, within Shewana3_4293 at 176.999 kb on - strand, within Shewana3_4293 at 177.001 kb on + strand, within Shewana3_4293 at 177.153 kb on + strand at 177.161 kb on + strand at 177.234 kb on + strand at 177.234 kb on + strand at 177.254 kb on - strand at 177.349 kb on + strand at 177.373 kb on + strand at 177.373 kb on + strand at 177.373 kb on + strand at 177.373 kb on + strand at 177.381 kb on - strand at 177.381 kb on - strand at 177.381 kb on - strand at 177.381 kb on - strand at 177.391 kb on - strand at 177.391 kb on - strand at 177.391 kb on - strand at 177.563 kb on - strand at 177.563 kb on - strand at 177.656 kb on + strand at 177.664 kb on - strand, within Shewana3_4294 at 177.817 kb on + strand, within Shewana3_4294 at 177.928 kb on - strand at 178.039 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 174,985 - -0.6 175,005 - -0.5 175,025 - Shewana3_4292 0.10 +0.5 175,025 - Shewana3_4292 0.10 +0.3 175,027 + Shewana3_4292 0.11 +1.9 175,115 + Shewana3_4292 0.27 -0.5 175,161 - Shewana3_4292 0.35 -0.2 175,168 + +0.9 175,212 + Shewana3_4292 0.45 +0.9 175,230 - Shewana3_4292 0.48 -1.3 175,252 + Shewana3_4292 0.52 +0.9 175,252 + Shewana3_4292 0.52 -0.7 175,252 + Shewana3_4292 0.52 -0.9 175,252 + Shewana3_4292 0.52 -1.4 175,260 - Shewana3_4292 0.53 -0.6 175,306 + Shewana3_4292 0.62 +1.9 175,388 + Shewana3_4292 0.77 +0.3 175,396 - Shewana3_4292 0.78 -0.5 175,411 + Shewana3_4292 0.81 -0.7 175,421 - Shewana3_4292 0.83 +0.4 175,466 + +0.3 175,515 + -2.1 175,523 - -1.1 175,528 - -0.4 175,608 - +0.1 175,637 - +0.5 175,683 - +0.2 175,685 + -1.8 175,691 - +1.6 175,697 - -1.6 175,710 + -0.8 175,718 - +1.0 175,993 + +0.1 176,001 - +0.9 176,003 + -1.1 176,011 - -2.3 176,036 + +0.9 176,059 - -1.1 176,072 - +0.2 176,177 + Shewana3_4293 0.17 -2.4 176,190 + Shewana3_4293 0.18 -1.4 176,196 + Shewana3_4293 0.19 -0.7 176,197 + Shewana3_4293 0.19 +1.7 176,197 + Shewana3_4293 0.19 -0.9 176,197 + Shewana3_4293 0.19 -0.8 176,197 + Shewana3_4293 0.19 -2.8 176,197 + Shewana3_4293 0.19 -1.5 176,197 + Shewana3_4293 0.19 -0.6 176,198 - Shewana3_4293 0.19 +0.3 176,228 + Shewana3_4293 0.21 +0.1 176,262 - Shewana3_4293 0.24 +1.9 176,267 - Shewana3_4293 0.25 -0.6 176,268 - Shewana3_4293 0.25 -1.9 176,309 + Shewana3_4293 0.28 -0.6 176,515 + Shewana3_4293 0.45 +0.8 176,681 + Shewana3_4293 0.59 -1.8 176,759 + Shewana3_4293 0.66 -0.7 176,874 - +1.9 176,875 + Shewana3_4293 0.76 -1.4 176,876 + Shewana3_4293 0.76 -0.2 176,876 + Shewana3_4293 0.76 -0.7 176,888 + Shewana3_4293 0.77 +0.3 176,896 - Shewana3_4293 0.77 +0.3 176,910 - Shewana3_4293 0.78 -2.4 176,943 + Shewana3_4293 0.81 +0.2 176,951 - Shewana3_4293 0.82 -0.1 176,951 - Shewana3_4293 0.82 +1.9 176,999 - Shewana3_4293 0.86 -0.7 177,001 + Shewana3_4293 0.86 -0.1 177,153 + +0.1 177,161 + +0.4 177,234 + -0.6 177,234 + -0.5 177,254 - +0.5 177,349 + +0.3 177,373 + -0.3 177,373 + -0.6 177,373 + -0.5 177,373 + -0.9 177,381 - +0.9 177,381 - -1.3 177,381 - +0.9 177,381 - +0.1 177,391 - +1.9 177,391 - +0.6 177,391 - -1.7 177,563 - +0.3 177,563 - -1.9 177,656 + -0.6 177,664 - Shewana3_4294 0.11 -0.7 177,817 + Shewana3_4294 0.65 -0.4 177,928 - -0.0 178,039 + -2.5
Or see this region's nucleotide sequence