Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4241 and Shewana3_4242 overlap by 1 nucleotides Shewana3_4242 and Shewana3_4243 are separated by 11 nucleotides Shewana3_4243 and Shewana3_4244 are separated by 17 nucleotides
Shewana3_4241: Shewana3_4241 - restriction modification system DNA specificity subunit (RefSeq), at 114,945 to 116,180
_4241
Shewana3_4242: Shewana3_4242 - hypothetical protein (RefSeq), at 116,180 to 116,785
_4242
Shewana3_4243: Shewana3_4243 - hypothetical protein (RefSeq), at 116,797 to 117,114
_4243
Shewana3_4244: Shewana3_4244 - HsdR family type I site-specific deoxyribonuclease (RefSeq), at 117,132 to 120,386
_4244
Position (kb) 116
117 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 115.239 kb on + strand, within Shewana3_4241 at 115.239 kb on + strand, within Shewana3_4241 at 115.314 kb on - strand, within Shewana3_4241 at 115.372 kb on + strand, within Shewana3_4241 at 115.373 kb on - strand, within Shewana3_4241 at 115.382 kb on - strand, within Shewana3_4241 at 115.477 kb on + strand, within Shewana3_4241 at 115.499 kb on - strand, within Shewana3_4241 at 115.709 kb on + strand, within Shewana3_4241 at 115.835 kb on - strand, within Shewana3_4241 at 115.840 kb on - strand, within Shewana3_4241 at 115.840 kb on - strand, within Shewana3_4241 at 115.845 kb on + strand, within Shewana3_4241 at 115.941 kb on + strand, within Shewana3_4241 at 115.998 kb on + strand, within Shewana3_4241 at 116.183 kb on - strand at 116.251 kb on - strand, within Shewana3_4242 at 116.280 kb on - strand, within Shewana3_4242 at 116.285 kb on - strand, within Shewana3_4242 at 116.527 kb on - strand, within Shewana3_4242 at 116.607 kb on + strand, within Shewana3_4242 at 116.665 kb on + strand, within Shewana3_4242 at 116.721 kb on + strand, within Shewana3_4242 at 116.721 kb on + strand, within Shewana3_4242 at 116.856 kb on + strand, within Shewana3_4243 at 116.866 kb on + strand, within Shewana3_4243 at 116.918 kb on + strand, within Shewana3_4243 at 117.050 kb on + strand, within Shewana3_4243 at 117.060 kb on + strand, within Shewana3_4243 at 117.068 kb on - strand, within Shewana3_4243 at 117.153 kb on + strand at 117.158 kb on + strand at 117.180 kb on - strand at 117.294 kb on - strand at 117.302 kb on - strand at 117.361 kb on + strand at 117.361 kb on + strand at 117.361 kb on + strand at 117.369 kb on - strand at 117.369 kb on - strand at 117.371 kb on + strand at 117.423 kb on + strand at 117.565 kb on + strand, within Shewana3_4244 at 117.565 kb on + strand, within Shewana3_4244 at 117.586 kb on - strand, within Shewana3_4244 at 117.593 kb on - strand, within Shewana3_4244 at 117.656 kb on - strand, within Shewana3_4244 at 117.667 kb on - strand, within Shewana3_4244
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 115,239 + Shewana3_4241 0.24 +0.2 115,239 + Shewana3_4241 0.24 +0.4 115,314 - Shewana3_4241 0.30 -0.2 115,372 + Shewana3_4241 0.35 -1.2 115,373 - Shewana3_4241 0.35 -1.4 115,382 - Shewana3_4241 0.35 +0.2 115,477 + Shewana3_4241 0.43 -0.1 115,499 - Shewana3_4241 0.45 -1.3 115,709 + Shewana3_4241 0.62 +3.8 115,835 - Shewana3_4241 0.72 -1.1 115,840 - Shewana3_4241 0.72 -0.3 115,840 - Shewana3_4241 0.72 -1.2 115,845 + Shewana3_4241 0.73 +0.6 115,941 + Shewana3_4241 0.81 +0.8 115,998 + Shewana3_4241 0.85 +0.5 116,183 - -3.0 116,251 - Shewana3_4242 0.12 -0.4 116,280 - Shewana3_4242 0.17 -0.7 116,285 - Shewana3_4242 0.17 -2.1 116,527 - Shewana3_4242 0.57 +0.9 116,607 + Shewana3_4242 0.70 +0.6 116,665 + Shewana3_4242 0.80 -0.1 116,721 + Shewana3_4242 0.89 +0.6 116,721 + Shewana3_4242 0.89 +1.0 116,856 + Shewana3_4243 0.19 +0.3 116,866 + Shewana3_4243 0.22 +0.3 116,918 + Shewana3_4243 0.38 +0.2 117,050 + Shewana3_4243 0.80 +0.2 117,060 + Shewana3_4243 0.83 +0.2 117,068 - Shewana3_4243 0.85 -0.0 117,153 + -1.2 117,158 + -0.6 117,180 - -0.1 117,294 - -2.5 117,302 - -0.9 117,361 + -2.2 117,361 + -0.4 117,361 + -0.0 117,369 - -1.4 117,369 - +0.3 117,371 + -0.2 117,423 + -1.1 117,565 + Shewana3_4244 0.13 +0.1 117,565 + Shewana3_4244 0.13 +0.6 117,586 - Shewana3_4244 0.14 -0.1 117,593 - Shewana3_4244 0.14 -1.4 117,656 - Shewana3_4244 0.16 +0.6 117,667 - Shewana3_4244 0.16 -0.2
Or see this region's nucleotide sequence