Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4160 and Shewana3_4161 overlap by 4 nucleotides Shewana3_4161 and Shewana3_4162 are separated by 7 nucleotides
Shewana3_4160: Shewana3_4160 - S-adenosylhomocysteine hydrolase (RefSeq), at 21,935 to 22,345
_4160
Shewana3_4161: Shewana3_4161 - hypothetical protein (RefSeq), at 22,342 to 23,283
_4161
Shewana3_4162: Shewana3_4162 - type I restriction-modification system, M subunit (RefSeq), at 23,291 to 25,915
_4162
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-2
-1
0
1 at 21.356 kb on - strand at 21.377 kb on - strand at 21.458 kb on - strand at 21.537 kb on + strand at 21.633 kb on + strand at 21.650 kb on + strand at 21.716 kb on - strand at 21.716 kb on - strand at 21.895 kb on - strand at 21.901 kb on - strand at 22.613 kb on - strand, within Shewana3_4161 at 22.766 kb on + strand, within Shewana3_4161 at 22.836 kb on + strand, within Shewana3_4161 at 22.848 kb on + strand, within Shewana3_4161 at 22.915 kb on + strand, within Shewana3_4161 at 23.000 kb on + strand, within Shewana3_4161 at 23.456 kb on - strand at 23.458 kb on + strand at 23.458 kb on - strand at 23.492 kb on + strand at 23.571 kb on + strand, within Shewana3_4162 at 23.848 kb on + strand, within Shewana3_4162 at 24.053 kb on - strand, within Shewana3_4162 at 24.120 kb on - strand, within Shewana3_4162 at 24.165 kb on + strand, within Shewana3_4162 at 24.206 kb on - strand, within Shewana3_4162 at 24.242 kb on - strand, within Shewana3_4162
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 21,356 - -0.2 21,377 - -0.2 21,458 - -0.0 21,537 + +0.0 21,633 + -0.3 21,650 + +0.2 21,716 - -0.3 21,716 - -0.4 21,895 - -2.3 21,901 - +0.4 22,613 - Shewana3_4161 0.29 -2.0 22,766 + Shewana3_4161 0.45 -0.6 22,836 + Shewana3_4161 0.52 -0.8 22,848 + Shewana3_4161 0.54 -1.5 22,915 + Shewana3_4161 0.61 -0.1 23,000 + Shewana3_4161 0.70 -1.2 23,456 - -1.3 23,458 + +1.3 23,458 - -1.6 23,492 + -1.9 23,571 + Shewana3_4162 0.11 -0.1 23,848 + Shewana3_4162 0.21 -2.0 24,053 - Shewana3_4162 0.29 -0.9 24,120 - Shewana3_4162 0.32 -0.7 24,165 + Shewana3_4162 0.33 +0.3 24,206 - Shewana3_4162 0.35 +0.3 24,242 - Shewana3_4162 0.36 +0.3
Or see this region's nucleotide sequence