Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4149 and Shewana3_4150 are separated by 130 nucleotides Shewana3_4150 and Shewana3_4151 are separated by 375 nucleotides Shewana3_4151 and Shewana3_4152 are separated by 41 nucleotides Shewana3_4152 and Shewana3_4153 are separated by 4 nucleotides Shewana3_4153 and Shewana3_4154 overlap by 4 nucleotides
Shewana3_4149: Shewana3_4149 - IS605 family transposase OrfB (RefSeq), at 10,342 to 11,445
_4149
Shewana3_4150: Shewana3_4150 - hypothetical protein (RefSeq), at 11,576 to 11,842
_4150
Shewana3_4151: Shewana3_4151 - hypothetical protein (RefSeq), at 12,218 to 12,505
_4151
Shewana3_4152: Shewana3_4152 - lipoprotein signal peptidase-like protein (RefSeq), at 12,547 to 12,888
_4152
Shewana3_4153: Shewana3_4153 - nitrogen regulatory protein P-II (RefSeq), at 12,893 to 13,294
_4153
Shewana3_4154: Shewana3_4154 - CzcA family heavy metal efflux protein (RefSeq), at 13,291 to 16,431
_4154
Position (kb) 12
13 Strain fitness (log2 ratio) -1
0
1
2 at 11.505 kb on + strand at 11.801 kb on + strand, within Shewana3_4150 at 11.806 kb on + strand, within Shewana3_4150 at 12.292 kb on - strand, within Shewana3_4151 at 12.542 kb on - strand at 12.666 kb on - strand, within Shewana3_4152 at 12.717 kb on - strand, within Shewana3_4152 at 12.941 kb on - strand, within Shewana3_4153 at 13.038 kb on - strand, within Shewana3_4153 at 13.066 kb on + strand, within Shewana3_4153 at 13.090 kb on + strand, within Shewana3_4153 at 13.356 kb on + strand at 13.392 kb on + strand at 13.482 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 11,505 + +1.4 11,801 + Shewana3_4150 0.84 +0.2 11,806 + Shewana3_4150 0.86 -0.2 12,292 - Shewana3_4151 0.26 +1.8 12,542 - -1.4 12,666 - Shewana3_4152 0.35 -0.7 12,717 - Shewana3_4152 0.50 +0.6 12,941 - Shewana3_4153 0.12 +0.1 13,038 - Shewana3_4153 0.36 -0.2 13,066 + Shewana3_4153 0.43 +0.6 13,090 + Shewana3_4153 0.49 +0.4 13,356 + -0.1 13,392 + -0.7 13,482 - -0.3
Or see this region's nucleotide sequence