Experiment: Ceftazidime 5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3221 and Shew_3222 are separated by 72 nucleotides Shew_3222 and Shew_3223 are separated by 175 nucleotides Shew_3223 and Shew_3224 are separated by 3 nucleotides Shew_3224 and Shew_3225 are separated by 38 nucleotides
Shew_3221: Shew_3221 - hypothetical protein (RefSeq), at 3,844,542 to 3,845,702
_3221
Shew_3222: Shew_3222 - protein of unknown function DUF335, SprT (RefSeq), at 3,845,775 to 3,846,386
_3222
Shew_3223: Shew_3223 - deoxyribonuclease I (RefSeq), at 3,846,562 to 3,847,317
_3223
Shew_3224: Shew_3224 - hypothetical protein (RefSeq), at 3,847,321 to 3,848,052
_3224
Shew_3225: Shew_3225 - glutathione synthetase (RefSeq), at 3,848,091 to 3,849,038
_3225
Position (kb)
3846
3847
3848 Strain fitness (log2 ratio)
-1
0
1 at 3846.041 kb on + strand, within Shew_3222 at 3846.089 kb on + strand, within Shew_3222 at 3846.202 kb on + strand, within Shew_3222 at 3846.237 kb on + strand, within Shew_3222 at 3846.670 kb on - strand, within Shew_3223 at 3847.082 kb on - strand, within Shew_3223 at 3847.254 kb on + strand at 3847.275 kb on - strand at 3847.352 kb on + strand at 3847.733 kb on + strand, within Shew_3224 at 3847.836 kb on + strand, within Shew_3224 at 3847.836 kb on + strand, within Shew_3224 at 3847.844 kb on - strand, within Shew_3224 at 3847.844 kb on - strand, within Shew_3224 at 3847.844 kb on - strand, within Shew_3224 at 3847.934 kb on + strand, within Shew_3224 at 3847.997 kb on - strand at 3848.197 kb on + strand, within Shew_3225
Per-strain Table
Position Strand Gene LocusTag Fraction Ceftazidime 5 mg/ml remove 3,846,041 + Shew_3222 0.43 +0.2 3,846,089 + Shew_3222 0.51 +0.8 3,846,202 + Shew_3222 0.70 -0.1 3,846,237 + Shew_3222 0.75 -0.8 3,846,670 - Shew_3223 0.14 -1.3 3,847,082 - Shew_3223 0.69 -1.3 3,847,254 + -0.6 3,847,275 - -0.8 3,847,352 + -0.1 3,847,733 + Shew_3224 0.56 -0.4 3,847,836 + Shew_3224 0.70 -0.1 3,847,836 + Shew_3224 0.70 -0.5 3,847,844 - Shew_3224 0.71 -0.8 3,847,844 - Shew_3224 0.71 -1.0 3,847,844 - Shew_3224 0.71 -0.8 3,847,934 + Shew_3224 0.84 -0.3 3,847,997 - -0.9 3,848,197 + Shew_3225 0.11 -0.0
Or see this region's nucleotide sequence