Experiment: Ceftazidime 5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt clpX and clpP are separated by 84 nucleotides clpP and tig are separated by 89 nucleotides tig and Shew_R0087 are separated by 252 nucleotides Shew_R0087 and Shew_R0088 are separated by 8 nucleotides Shew_R0088 and Shew_R0089 are separated by 63 nucleotides Shew_R0089 and Shew_2510 are separated by 347 nucleotides
Shew_2507: clpX - ATP-dependent protease ATP-binding subunit (RefSeq), at 2,949,498 to 2,950,778
clpX
Shew_2508: clpP - ATP-dependent Clp protease proteolytic subunit (RefSeq), at 2,950,863 to 2,951,474
clpP
Shew_2509: tig - trigger factor (RefSeq), at 2,951,564 to 2,952,868
tig
Shew_R0087: Shew_R0087 - tRNA-His (RefSeq), at 2,953,121 to 2,953,196
_R0087
Shew_R0088: Shew_R0088 - tRNA-Arg (RefSeq), at 2,953,205 to 2,953,281
_R0088
Shew_R0089: Shew_R0089 - tRNA-Pro (RefSeq), at 2,953,345 to 2,953,421
_R0089
Shew_2510: Shew_2510 - methylenetetrahydrofolate dehydrogenase (NADP(+)) (RefSeq), at 2,953,769 to 2,954,623
_2510
Position (kb)
2951
2952
2953 Strain fitness (log2 ratio)
-1
0
1 at 2950.980 kb on - strand, within clpP at 2951.450 kb on - strand at 2951.795 kb on - strand, within tig at 2951.867 kb on - strand, within tig at 2952.413 kb on - strand, within tig at 2952.508 kb on + strand, within tig at 2952.840 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ceftazidime 5 mg/ml remove 2,950,980 - clpP Shew_2508 0.19 +0.9 2,951,450 - -0.1 2,951,795 - tig Shew_2509 0.18 -0.6 2,951,867 - tig Shew_2509 0.23 -0.7 2,952,413 - tig Shew_2509 0.65 -0.9 2,952,508 + tig Shew_2509 0.72 -1.4 2,952,840 - -0.9
Or see this region's nucleotide sequence