Experiment: Ceftazidime 5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2347 and Shew_2348 are separated by 239 nucleotides Shew_2348 and Shew_2349 overlap by 10 nucleotides Shew_2349 and Shew_2350 overlap by 4 nucleotides Shew_2350 and Shew_2351 are separated by 120 nucleotides
Shew_2347: Shew_2347 - phosphonate ABC transporter, periplasmic phosphonate-binding protein (RefSeq), at 2,741,403 to 2,742,257
_2347
Shew_2348: Shew_2348 - two component transcriptional regulator (RefSeq), at 2,742,497 to 2,743,171
_2348
Shew_2349: Shew_2349 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 2,743,162 to 2,744,511
_2349
Shew_2350: Shew_2350 - 2OG-Fe(II) oxygenase (RefSeq), at 2,744,508 to 2,745,203
_2350
Shew_2351: Shew_2351 - metal dependent phosphohydrolase (RefSeq), at 2,745,324 to 2,746,589
_2351
Position (kb)
2743
2744
2745 Strain fitness (log2 ratio)
-2
-1
0
1 at 2742.252 kb on + strand at 2742.394 kb on - strand at 2742.549 kb on - strand at 2743.389 kb on + strand, within Shew_2349 at 2743.397 kb on - strand, within Shew_2349 at 2743.487 kb on - strand, within Shew_2349 at 2743.866 kb on - strand, within Shew_2349 at 2744.124 kb on + strand, within Shew_2349 at 2745.041 kb on + strand, within Shew_2350 at 2745.132 kb on - strand, within Shew_2350 at 2745.407 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ceftazidime 5 mg/ml remove 2,742,252 + -2.1 2,742,394 - -0.7 2,742,549 - -1.4 2,743,389 + Shew_2349 0.17 -0.9 2,743,397 - Shew_2349 0.17 -1.0 2,743,487 - Shew_2349 0.24 -1.2 2,743,866 - Shew_2349 0.52 -0.2 2,744,124 + Shew_2349 0.71 -2.0 2,745,041 + Shew_2350 0.77 +0.4 2,745,132 - Shew_2350 0.90 +0.5 2,745,407 - +0.6
Or see this region's nucleotide sequence