Experiment: Ceftazidime 5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaA and Shew_1299 are separated by 146 nucleotides Shew_1299 and Shew_1300 are separated by 233 nucleotides Shew_1300 and Shew_1301 are separated by 325 nucleotides
Shew_1298: guaA - bifunctional GMP synthase/glutamine amidotransferase protein (RefSeq), at 1,500,113 to 1,501,690
guaA
Shew_1299: Shew_1299 - CMP/dCMP deaminase, zinc-binding (RefSeq), at 1,501,837 to 1,502,373
_1299
Shew_1300: Shew_1300 - lytic transglycosylase, catalytic (RefSeq), at 1,502,607 to 1,504,037
_1300
Shew_1301: Shew_1301 - phosphoribosylformylglycinamidine synthase (RefSeq), at 1,504,363 to 1,508,244
_1301
Position (kb)
1502
1503
1504
1505 Strain fitness (log2 ratio)
-1
0
1 at 1501.791 kb on + strand at 1502.544 kb on - strand at 1502.566 kb on + strand at 1502.566 kb on + strand at 1502.676 kb on - strand at 1502.866 kb on - strand, within Shew_1300 at 1503.343 kb on + strand, within Shew_1300 at 1503.348 kb on - strand, within Shew_1300 at 1503.404 kb on + strand, within Shew_1300 at 1503.711 kb on - strand, within Shew_1300 at 1503.758 kb on - strand, within Shew_1300 at 1503.895 kb on + strand at 1503.925 kb on - strand at 1504.265 kb on + strand at 1504.427 kb on + strand at 1504.546 kb on - strand at 1504.578 kb on + strand at 1504.836 kb on - strand, within Shew_1301 at 1504.883 kb on + strand, within Shew_1301 at 1504.883 kb on + strand, within Shew_1301
Per-strain Table
Position Strand Gene LocusTag Fraction Ceftazidime 5 mg/ml remove 1,501,791 + +0.5 1,502,544 - -0.5 1,502,566 + -0.5 1,502,566 + -0.6 1,502,676 - -0.2 1,502,866 - Shew_1300 0.18 +0.5 1,503,343 + Shew_1300 0.51 -1.4 1,503,348 - Shew_1300 0.52 -0.4 1,503,404 + Shew_1300 0.56 -1.3 1,503,711 - Shew_1300 0.77 -1.6 1,503,758 - Shew_1300 0.80 -0.3 1,503,895 + -0.1 1,503,925 - +1.6 1,504,265 + +0.6 1,504,427 + +0.1 1,504,546 - +0.5 1,504,578 + +0.6 1,504,836 - Shew_1301 0.12 +0.2 1,504,883 + Shew_1301 0.13 +0.3 1,504,883 + Shew_1301 0.13 -0.9
Or see this region's nucleotide sequence