Strain Fitness in Shewanella loihica PV-4 around Shew_0185

Experiment: Ceftazidime 5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShew_0183 and rplQ are separated by 39 nucleotidesrplQ and Shew_0185 are separated by 56 nucleotidesShew_0185 and Shew_0186 are separated by 134 nucleotidesShew_0186 and Shew_0187 overlap by 4 nucleotidesShew_0187 and Shew_0188 overlap by 1 nucleotides Shew_0183: Shew_0183 - DNA-directed RNA polymerase subunit alpha (RefSeq), at 191,415 to 192,404 _0183 Shew_0184: rplQ - 50S ribosomal protein L17 (RefSeq), at 192,444 to 192,836 rplQ Shew_0185: Shew_0185 - hypothetical protein (RefSeq), at 192,893 to 193,870 _0185 Shew_0186: Shew_0186 - cytochrome c-type biogenesis protein CcmE (RefSeq), at 194,005 to 194,490 _0186 Shew_0187: Shew_0187 - Heme exporter protein D (CcmD) (RefSeq), at 194,487 to 194,687 _0187 Shew_0188: Shew_0188 - heme exporter protein CcmC (RefSeq), at 194,687 to 195,433 _0188 Position (kb) 192 193 194Strain fitness (log2 ratio) -1 0 1at 193.143 kb on + strand, within Shew_0185at 193.149 kb on - strand, within Shew_0185at 193.440 kb on - strand, within Shew_0185at 193.458 kb on + strand, within Shew_0185at 193.466 kb on - strand, within Shew_0185at 193.772 kb on - strand, within Shew_0185at 193.974 kb on + strandat 194.030 kb on - strandat 194.050 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ceftazidime 5 mg/ml
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193,143 + Shew_0185 0.26 +0.3
193,149 - Shew_0185 0.26 +1.0
193,440 - Shew_0185 0.56 +0.4
193,458 + Shew_0185 0.58 +0.6
193,466 - Shew_0185 0.59 +0.9
193,772 - Shew_0185 0.90 +0.3
193,974 + -0.1
194,030 - +0.1
194,050 + +0.2

Or see this region's nucleotide sequence