Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_786

Experiment: L-Lysine 25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_784 and Pf6N2E2_785 are separated by 37 nucleotidesPf6N2E2_785 and Pf6N2E2_786 are separated by 109 nucleotidesPf6N2E2_786 and Pf6N2E2_787 are separated by 253 nucleotides Pf6N2E2_784 - Outer membrane usher protein FIMD, at 897,590 to 900,085 _784 Pf6N2E2_785 - P pilus assembly protein, chaperone PapD, at 900,123 to 900,884 _785 Pf6N2E2_786 - Type-1 fimbrial protein, A chain precursor, at 900,994 to 901,542 _786 Pf6N2E2_787 - YD repeat protein, at 901,796 to 906,883 _787 Position (kb) 900 901 902Strain fitness (log2 ratio) -1 0 1 2at 900.098 kb on + strandat 900.278 kb on + strand, within Pf6N2E2_785at 900.279 kb on - strand, within Pf6N2E2_785at 900.352 kb on + strand, within Pf6N2E2_785at 900.352 kb on + strand, within Pf6N2E2_785at 900.352 kb on + strand, within Pf6N2E2_785at 900.479 kb on + strand, within Pf6N2E2_785at 900.535 kb on + strand, within Pf6N2E2_785at 900.535 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.700 kb on - strand, within Pf6N2E2_785at 900.700 kb on - strand, within Pf6N2E2_785at 900.701 kb on + strand, within Pf6N2E2_785at 900.892 kb on + strandat 900.958 kb on + strandat 901.116 kb on - strand, within Pf6N2E2_786at 901.255 kb on - strand, within Pf6N2E2_786at 901.377 kb on - strand, within Pf6N2E2_786at 901.377 kb on - strand, within Pf6N2E2_786at 901.377 kb on - strand, within Pf6N2E2_786at 901.377 kb on - strand, within Pf6N2E2_786at 901.377 kb on - strand, within Pf6N2E2_786at 901.638 kb on + strandat 901.644 kb on + strandat 901.644 kb on + strandat 901.877 kb on + strandat 902.020 kb on + strandat 902.030 kb on + strandat 902.031 kb on - strandat 902.042 kb on - strandat 902.042 kb on - strandat 902.051 kb on + strandat 902.052 kb on - strandat 902.052 kb on - strandat 902.052 kb on - strandat 902.055 kb on - strandat 902.176 kb on - strandat 902.176 kb on - strandat 902.207 kb on + strandat 902.208 kb on - strandat 902.275 kb on + strandat 902.276 kb on - strandat 902.276 kb on - strandat 902.276 kb on - strandat 902.303 kb on + strandat 902.304 kb on - strandat 902.304 kb on - strandat 902.304 kb on - strandat 902.305 kb on + strand, within Pf6N2E2_787at 902.305 kb on + strand, within Pf6N2E2_787at 902.306 kb on - strand, within Pf6N2E2_787at 902.306 kb on - strand, within Pf6N2E2_787at 902.311 kb on - strand, within Pf6N2E2_787at 902.311 kb on - strand, within Pf6N2E2_787at 902.311 kb on - strand, within Pf6N2E2_787at 902.335 kb on + strand, within Pf6N2E2_787at 902.335 kb on + strand, within Pf6N2E2_787at 902.360 kb on + strand, within Pf6N2E2_787at 902.361 kb on - strand, within Pf6N2E2_787at 902.389 kb on + strand, within Pf6N2E2_787at 902.419 kb on + strand, within Pf6N2E2_787at 902.419 kb on + strand, within Pf6N2E2_787at 902.420 kb on - strand, within Pf6N2E2_787at 902.439 kb on - strand, within Pf6N2E2_787at 902.457 kb on - strand, within Pf6N2E2_787at 902.457 kb on - strand, within Pf6N2E2_787at 902.457 kb on - strand, within Pf6N2E2_787at 902.457 kb on - strand, within Pf6N2E2_787at 902.519 kb on - strand, within Pf6N2E2_787at 902.519 kb on - strand, within Pf6N2E2_787

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lysine 25 mM
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900,098 + -1.0
900,278 + Pf6N2E2_785 0.20 -0.1
900,279 - Pf6N2E2_785 0.20 -1.5
900,352 + Pf6N2E2_785 0.30 +0.2
900,352 + Pf6N2E2_785 0.30 -0.0
900,352 + Pf6N2E2_785 0.30 -1.6
900,479 + Pf6N2E2_785 0.47 +0.0
900,535 + Pf6N2E2_785 0.54 -1.0
900,535 + Pf6N2E2_785 0.54 -0.3
900,699 + Pf6N2E2_785 0.76 -0.3
900,699 + Pf6N2E2_785 0.76 +0.1
900,699 + Pf6N2E2_785 0.76 -0.4
900,699 + Pf6N2E2_785 0.76 -0.2
900,699 + Pf6N2E2_785 0.76 -0.6
900,700 - Pf6N2E2_785 0.76 -0.3
900,700 - Pf6N2E2_785 0.76 -0.1
900,701 + Pf6N2E2_785 0.76 -1.0
900,892 + -0.2
900,958 + +0.4
901,116 - Pf6N2E2_786 0.22 +1.2
901,255 - Pf6N2E2_786 0.48 +0.3
901,377 - Pf6N2E2_786 0.70 +0.7
901,377 - Pf6N2E2_786 0.70 -0.4
901,377 - Pf6N2E2_786 0.70 +0.4
901,377 - Pf6N2E2_786 0.70 +0.8
901,377 - Pf6N2E2_786 0.70 +0.4
901,638 + -1.5
901,644 + +1.3
901,644 + -1.7
901,877 + +1.2
902,020 + +0.3
902,030 + +1.2
902,031 - +0.1
902,042 - +0.4
902,042 - -0.4
902,051 + -0.7
902,052 - -0.7
902,052 - +0.3
902,052 - -0.0
902,055 - +0.3
902,176 - -0.2
902,176 - +0.4
902,207 + +0.8
902,208 - -0.0
902,275 + -0.0
902,276 - -0.2
902,276 - +0.3
902,276 - -0.8
902,303 + -1.2
902,304 - +0.3
902,304 - -0.7
902,304 - +2.0
902,305 + Pf6N2E2_787 0.10 +0.3
902,305 + Pf6N2E2_787 0.10 -1.0
902,306 - Pf6N2E2_787 0.10 +0.2
902,306 - Pf6N2E2_787 0.10 -1.1
902,311 - Pf6N2E2_787 0.10 +1.5
902,311 - Pf6N2E2_787 0.10 +1.0
902,311 - Pf6N2E2_787 0.10 -0.8
902,335 + Pf6N2E2_787 0.11 -0.8
902,335 + Pf6N2E2_787 0.11 +0.7
902,360 + Pf6N2E2_787 0.11 -0.1
902,361 - Pf6N2E2_787 0.11 -0.8
902,389 + Pf6N2E2_787 0.12 -0.7
902,419 + Pf6N2E2_787 0.12 +0.2
902,419 + Pf6N2E2_787 0.12 +0.2
902,420 - Pf6N2E2_787 0.12 -0.3
902,439 - Pf6N2E2_787 0.13 -0.1
902,457 - Pf6N2E2_787 0.13 +0.3
902,457 - Pf6N2E2_787 0.13 -0.6
902,457 - Pf6N2E2_787 0.13 +0.3
902,457 - Pf6N2E2_787 0.13 +0.3
902,519 - Pf6N2E2_787 0.14 +0.4
902,519 - Pf6N2E2_787 0.14 +0.3

Or see this region's nucleotide sequence