Experiment: L-Lysine 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_697 and Pf6N2E2_698 are separated by 28 nucleotides Pf6N2E2_698 and Pf6N2E2_699 overlap by 26 nucleotides Pf6N2E2_699 and Pf6N2E2_700 overlap by 8 nucleotides Pf6N2E2_700 and Pf6N2E2_701 overlap by 4 nucleotides Pf6N2E2_701 and Pf6N2E2_702 are separated by 2 nucleotides
Pf6N2E2_697 - Heme d1 biosynthesis protein NirJ, at 803,891 to 805,069
_697
Pf6N2E2_698 - Heme d1 biosynthesis protein NirH, at 805,098 to 805,634
_698
Pf6N2E2_699 - Heme d1 biosynthesis protein NirG, at 805,609 to 806,052
_699
Pf6N2E2_700 - Heme d1 biosynthesis protein NirL, at 806,045 to 806,557
_700
Pf6N2E2_701 - Heme d1 biosynthesis protein NirD / Heme d1 biosynthesis protein NirL, at 806,554 to 807,012
_701
Pf6N2E2_702 - Heme d1 biosynthesis protein NirF, at 807,015 to 808,193
_702
Position (kb) 806
807 Strain fitness (log2 ratio) -2
-1
0
1 at 805.070 kb on - strand at 805.070 kb on - strand at 805.070 kb on - strand at 805.070 kb on - strand at 805.210 kb on + strand, within Pf6N2E2_698 at 805.211 kb on - strand, within Pf6N2E2_698 at 805.623 kb on - strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.769 kb on + strand, within Pf6N2E2_699 at 805.769 kb on + strand, within Pf6N2E2_699 at 805.906 kb on + strand, within Pf6N2E2_699 at 805.906 kb on + strand, within Pf6N2E2_699 at 805.907 kb on - strand, within Pf6N2E2_699 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.224 kb on - strand, within Pf6N2E2_700 at 806.257 kb on - strand, within Pf6N2E2_700 at 806.708 kb on + strand, within Pf6N2E2_701 at 806.708 kb on + strand, within Pf6N2E2_701 at 806.709 kb on - strand, within Pf6N2E2_701 at 806.726 kb on + strand, within Pf6N2E2_701 at 806.726 kb on + strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 807.451 kb on + strand, within Pf6N2E2_702 at 807.452 kb on - strand, within Pf6N2E2_702
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 25 mM remove 805,070 - -0.8 805,070 - -0.1 805,070 - -0.3 805,070 - +0.4 805,210 + Pf6N2E2_698 0.21 -1.3 805,211 - Pf6N2E2_698 0.21 +0.4 805,623 - +1.1 805,637 + -1.6 805,637 + +1.4 805,637 + -0.6 805,637 + +0.3 805,637 + +1.1 805,637 + -0.9 805,637 + -0.8 805,637 + +0.2 805,638 - -0.8 805,638 - -2.7 805,638 - +0.1 805,638 - -0.5 805,638 - -0.5 805,638 - +1.1 805,638 - +1.1 805,638 - +0.9 805,638 - -0.6 805,638 - +0.4 805,769 + Pf6N2E2_699 0.36 -0.1 805,769 + Pf6N2E2_699 0.36 +0.6 805,906 + Pf6N2E2_699 0.67 +0.6 805,906 + Pf6N2E2_699 0.67 +0.5 805,907 - Pf6N2E2_699 0.67 +0.8 806,223 + Pf6N2E2_700 0.35 -0.5 806,223 + Pf6N2E2_700 0.35 -0.8 806,223 + Pf6N2E2_700 0.35 -0.7 806,223 + Pf6N2E2_700 0.35 -0.9 806,223 + Pf6N2E2_700 0.35 -0.9 806,223 + Pf6N2E2_700 0.35 -1.0 806,224 - Pf6N2E2_700 0.35 +0.3 806,257 - Pf6N2E2_700 0.41 -0.5 806,708 + Pf6N2E2_701 0.34 +0.1 806,708 + Pf6N2E2_701 0.34 +0.7 806,709 - Pf6N2E2_701 0.34 +0.2 806,726 + Pf6N2E2_701 0.37 -0.4 806,726 + Pf6N2E2_701 0.37 +0.7 806,727 - Pf6N2E2_701 0.38 +0.2 806,727 - Pf6N2E2_701 0.38 -1.9 806,727 - Pf6N2E2_701 0.38 +0.2 806,727 - Pf6N2E2_701 0.38 -0.2 807,451 + Pf6N2E2_702 0.37 -1.0 807,452 - Pf6N2E2_702 0.37 -0.3
Or see this region's nucleotide sequence