Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5719

Experiment: L-Lysine 25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5717 and Pf6N2E2_5718 overlap by 1 nucleotidesPf6N2E2_5718 and Pf6N2E2_5719 are separated by 335 nucleotidesPf6N2E2_5719 and Pf6N2E2_5720 are separated by 66 nucleotides Pf6N2E2_5717 - Heat shock protein, at 6,531,591 to 6,531,995 _5717 Pf6N2E2_5718 - FIG00953240: hypothetical protein, at 6,531,995 to 6,532,429 _5718 Pf6N2E2_5719 - D-lactate dehydrogenase (EC 1.1.1.28), at 6,532,765 to 6,533,754 _5719 Pf6N2E2_5720 - BarA sensory histidine kinase (= VarS = GacS), at 6,533,821 to 6,536,676 _5720 Position (kb) 6532 6533 6534Strain fitness (log2 ratio) -1 0 1at 6531.789 kb on - strand, within Pf6N2E2_5717at 6531.868 kb on + strand, within Pf6N2E2_5717at 6531.868 kb on + strand, within Pf6N2E2_5717at 6531.869 kb on - strand, within Pf6N2E2_5717at 6531.907 kb on + strand, within Pf6N2E2_5717at 6531.907 kb on + strand, within Pf6N2E2_5717at 6531.908 kb on - strand, within Pf6N2E2_5717at 6531.908 kb on - strand, within Pf6N2E2_5717at 6531.908 kb on - strand, within Pf6N2E2_5717at 6531.908 kb on - strand, within Pf6N2E2_5717at 6531.917 kb on + strand, within Pf6N2E2_5717at 6531.918 kb on - strand, within Pf6N2E2_5717at 6531.918 kb on - strand, within Pf6N2E2_5717at 6531.931 kb on - strand, within Pf6N2E2_5717at 6531.931 kb on - strand, within Pf6N2E2_5717at 6531.931 kb on - strand, within Pf6N2E2_5717at 6531.931 kb on - strand, within Pf6N2E2_5717at 6532.442 kb on + strandat 6532.442 kb on + strandat 6532.443 kb on - strandat 6532.452 kb on - strandat 6532.456 kb on + strandat 6532.456 kb on + strandat 6532.456 kb on + strandat 6532.457 kb on - strandat 6532.457 kb on - strandat 6532.485 kb on + strandat 6532.741 kb on + strandat 6532.742 kb on - strandat 6532.976 kb on + strand, within Pf6N2E2_5719at 6532.976 kb on + strand, within Pf6N2E2_5719at 6533.237 kb on + strand, within Pf6N2E2_5719at 6533.238 kb on - strand, within Pf6N2E2_5719at 6533.238 kb on - strand, within Pf6N2E2_5719at 6533.238 kb on - strand, within Pf6N2E2_5719at 6533.384 kb on + strand, within Pf6N2E2_5719at 6533.384 kb on + strand, within Pf6N2E2_5719at 6533.384 kb on + strand, within Pf6N2E2_5719at 6533.457 kb on - strand, within Pf6N2E2_5719at 6533.520 kb on - strand, within Pf6N2E2_5719at 6533.724 kb on - strandat 6533.776 kb on + strandat 6533.777 kb on - strandat 6533.832 kb on + strandat 6533.832 kb on + strandat 6533.832 kb on + strandat 6534.267 kb on - strand, within Pf6N2E2_5720at 6534.526 kb on + strand, within Pf6N2E2_5720at 6534.526 kb on + strand, within Pf6N2E2_5720at 6534.526 kb on + strand, within Pf6N2E2_5720at 6534.713 kb on + strand, within Pf6N2E2_5720at 6534.714 kb on - strand, within Pf6N2E2_5720at 6534.714 kb on - strand, within Pf6N2E2_5720at 6534.714 kb on - strand, within Pf6N2E2_5720at 6534.748 kb on + strand, within Pf6N2E2_5720at 6534.748 kb on + strand, within Pf6N2E2_5720at 6534.749 kb on - strand, within Pf6N2E2_5720at 6534.749 kb on - strand, within Pf6N2E2_5720

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lysine 25 mM
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6,531,789 - Pf6N2E2_5717 0.49 +0.5
6,531,868 + Pf6N2E2_5717 0.68 -0.5
6,531,868 + Pf6N2E2_5717 0.68 -0.0
6,531,869 - Pf6N2E2_5717 0.69 -0.7
6,531,907 + Pf6N2E2_5717 0.78 +0.6
6,531,907 + Pf6N2E2_5717 0.78 -0.3
6,531,908 - Pf6N2E2_5717 0.78 +0.4
6,531,908 - Pf6N2E2_5717 0.78 +0.8
6,531,908 - Pf6N2E2_5717 0.78 -0.1
6,531,908 - Pf6N2E2_5717 0.78 +0.6
6,531,917 + Pf6N2E2_5717 0.80 -0.0
6,531,918 - Pf6N2E2_5717 0.81 -0.3
6,531,918 - Pf6N2E2_5717 0.81 +1.1
6,531,931 - Pf6N2E2_5717 0.84 +0.7
6,531,931 - Pf6N2E2_5717 0.84 +0.1
6,531,931 - Pf6N2E2_5717 0.84 +0.9
6,531,931 - Pf6N2E2_5717 0.84 -0.5
6,532,442 + +0.8
6,532,442 + -0.1
6,532,443 - -0.9
6,532,452 - -0.4
6,532,456 + -0.1
6,532,456 + +0.0
6,532,456 + +0.3
6,532,457 - -0.6
6,532,457 - +0.7
6,532,485 + +0.8
6,532,741 + +1.3
6,532,742 - +0.8
6,532,976 + Pf6N2E2_5719 0.21 -0.8
6,532,976 + Pf6N2E2_5719 0.21 +1.3
6,533,237 + Pf6N2E2_5719 0.48 +0.5
6,533,238 - Pf6N2E2_5719 0.48 +0.4
6,533,238 - Pf6N2E2_5719 0.48 +0.6
6,533,238 - Pf6N2E2_5719 0.48 -0.0
6,533,384 + Pf6N2E2_5719 0.63 +0.5
6,533,384 + Pf6N2E2_5719 0.63 +1.2
6,533,384 + Pf6N2E2_5719 0.63 +1.7
6,533,457 - Pf6N2E2_5719 0.70 +0.8
6,533,520 - Pf6N2E2_5719 0.76 +0.3
6,533,724 - +0.5
6,533,776 + -0.0
6,533,777 - -0.4
6,533,832 + +1.5
6,533,832 + +0.2
6,533,832 + -0.3
6,534,267 - Pf6N2E2_5720 0.16 +1.1
6,534,526 + Pf6N2E2_5720 0.25 +0.9
6,534,526 + Pf6N2E2_5720 0.25 +1.1
6,534,526 + Pf6N2E2_5720 0.25 +1.1
6,534,713 + Pf6N2E2_5720 0.31 +0.8
6,534,714 - Pf6N2E2_5720 0.31 +1.0
6,534,714 - Pf6N2E2_5720 0.31 +0.5
6,534,714 - Pf6N2E2_5720 0.31 +0.7
6,534,748 + Pf6N2E2_5720 0.32 +0.8
6,534,748 + Pf6N2E2_5720 0.32 +1.0
6,534,749 - Pf6N2E2_5720 0.32 +0.5
6,534,749 - Pf6N2E2_5720 0.32 +1.2

Or see this region's nucleotide sequence