Experiment: L-Lysine 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5542 and Pf6N2E2_5543 are separated by 52 nucleotides Pf6N2E2_5543 and Pf6N2E2_5544 are separated by 81 nucleotides Pf6N2E2_5544 and Pf6N2E2_5545 are separated by 166 nucleotides Pf6N2E2_5545 and Pf6N2E2_5546 are separated by 1 nucleotides
Pf6N2E2_5542 - tolB protein precursor, periplasmic protein involved in the tonb-independent uptake of group A colicins, at 6,357,030 to 6,358,310
_5542
Pf6N2E2_5543 - 18K peptidoglycan-associated outer membrane lipoprotein; Peptidoglycan-associated lipoprotein precursor; Outer membrane protein P6; OmpA/MotB precursor, at 6,358,363 to 6,358,860
_5543
Pf6N2E2_5544 - TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain, at 6,358,942 to 6,359,706
_5544
Pf6N2E2_5545 - Queuosine Biosynthesis QueE Radical SAM, at 6,359,873 to 6,360,520
_5545
Pf6N2E2_5546 - Queuosine Biosynthesis QueC ATPase, at 6,360,522 to 6,361,214
_5546
Position (kb)
6358
6359
6360 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6358.299 kb on + strand at 6358.319 kb on + strand at 6358.321 kb on + strand at 6358.321 kb on + strand at 6358.321 kb on + strand at 6358.321 kb on + strand at 6358.328 kb on + strand at 6358.328 kb on + strand at 6358.332 kb on + strand at 6358.350 kb on + strand at 6358.350 kb on + strand at 6358.858 kb on + strand at 6358.858 kb on + strand at 6358.858 kb on + strand at 6358.889 kb on + strand at 6358.965 kb on + strand at 6358.965 kb on + strand at 6358.980 kb on + strand at 6359.192 kb on + strand, within Pf6N2E2_5544 at 6359.193 kb on - strand, within Pf6N2E2_5544 at 6359.350 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.353 kb on + strand, within Pf6N2E2_5544 at 6359.354 kb on - strand, within Pf6N2E2_5544 at 6359.431 kb on + strand, within Pf6N2E2_5544 at 6359.431 kb on + strand, within Pf6N2E2_5544 at 6359.431 kb on + strand, within Pf6N2E2_5544 at 6359.464 kb on + strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.575 kb on + strand, within Pf6N2E2_5544 at 6359.591 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.705 kb on - strand at 6359.705 kb on - strand at 6359.777 kb on - strand at 6359.850 kb on + strand at 6359.857 kb on + strand at 6359.858 kb on - strand at 6359.873 kb on - strand at 6359.949 kb on + strand, within Pf6N2E2_5545 at 6359.950 kb on - strand, within Pf6N2E2_5545 at 6359.950 kb on - strand, within Pf6N2E2_5545 at 6359.987 kb on + strand, within Pf6N2E2_5545 at 6360.063 kb on - strand, within Pf6N2E2_5545 at 6360.074 kb on + strand, within Pf6N2E2_5545 at 6360.075 kb on - strand, within Pf6N2E2_5545 at 6360.188 kb on - strand, within Pf6N2E2_5545 at 6360.362 kb on + strand, within Pf6N2E2_5545 at 6360.487 kb on + strand at 6360.487 kb on + strand at 6360.487 kb on + strand at 6360.488 kb on - strand at 6360.488 kb on - strand at 6360.491 kb on + strand at 6360.491 kb on + strand at 6360.491 kb on + strand at 6360.492 kb on - strand at 6360.521 kb on + strand at 6360.521 kb on + strand at 6360.522 kb on - strand at 6360.522 kb on - strand at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.649 kb on - strand, within Pf6N2E2_5546
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 25 mM remove 6,358,299 + +0.6 6,358,319 + -1.0 6,358,321 + -1.2 6,358,321 + +0.7 6,358,321 + -1.1 6,358,321 + +0.4 6,358,328 + -1.2 6,358,328 + -1.0 6,358,332 + -0.6 6,358,350 + -2.8 6,358,350 + -0.7 6,358,858 + -0.9 6,358,858 + -2.8 6,358,858 + -2.4 6,358,889 + -2.7 6,358,965 + -2.3 6,358,965 + -1.8 6,358,980 + -2.5 6,359,192 + Pf6N2E2_5544 0.33 -1.7 6,359,193 - Pf6N2E2_5544 0.33 -0.7 6,359,350 + Pf6N2E2_5544 0.53 -3.3 6,359,353 + Pf6N2E2_5544 0.54 -2.4 6,359,353 + Pf6N2E2_5544 0.54 -3.8 6,359,353 + Pf6N2E2_5544 0.54 -2.8 6,359,353 + Pf6N2E2_5544 0.54 -2.3 6,359,353 + Pf6N2E2_5544 0.54 -4.6 6,359,354 - Pf6N2E2_5544 0.54 -0.9 6,359,431 + Pf6N2E2_5544 0.64 -2.2 6,359,431 + Pf6N2E2_5544 0.64 -2.9 6,359,431 + Pf6N2E2_5544 0.64 -2.8 6,359,464 + Pf6N2E2_5544 0.68 -3.4 6,359,543 - Pf6N2E2_5544 0.79 -0.4 6,359,543 - Pf6N2E2_5544 0.79 -2.0 6,359,543 - Pf6N2E2_5544 0.79 -2.2 6,359,543 - Pf6N2E2_5544 0.79 -3.3 6,359,575 + Pf6N2E2_5544 0.83 -2.8 6,359,591 + Pf6N2E2_5544 0.85 -3.1 6,359,621 + Pf6N2E2_5544 0.89 -3.3 6,359,621 + Pf6N2E2_5544 0.89 -3.4 6,359,621 + Pf6N2E2_5544 0.89 -2.7 6,359,621 + Pf6N2E2_5544 0.89 -4.5 6,359,622 - Pf6N2E2_5544 0.89 -2.8 6,359,622 - Pf6N2E2_5544 0.89 -2.4 6,359,622 - Pf6N2E2_5544 0.89 -3.1 6,359,704 + +0.1 6,359,704 + -1.3 6,359,704 + -0.6 6,359,704 + -0.8 6,359,705 - +0.4 6,359,705 - -1.8 6,359,777 - -0.3 6,359,850 + +0.2 6,359,857 + -0.0 6,359,858 - +0.8 6,359,873 - +0.6 6,359,949 + Pf6N2E2_5545 0.12 +1.1 6,359,950 - Pf6N2E2_5545 0.12 -0.2 6,359,950 - Pf6N2E2_5545 0.12 +0.5 6,359,987 + Pf6N2E2_5545 0.18 +0.7 6,360,063 - Pf6N2E2_5545 0.29 +0.7 6,360,074 + Pf6N2E2_5545 0.31 +1.0 6,360,075 - Pf6N2E2_5545 0.31 +0.7 6,360,188 - Pf6N2E2_5545 0.49 +1.3 6,360,362 + Pf6N2E2_5545 0.75 -0.3 6,360,487 + +1.0 6,360,487 + +1.8 6,360,487 + +0.6 6,360,488 - -0.1 6,360,488 - +0.1 6,360,491 + +0.6 6,360,491 + +0.6 6,360,491 + +0.7 6,360,492 - +0.4 6,360,521 + -1.4 6,360,521 + +0.4 6,360,522 - +0.9 6,360,522 - +0.8 6,360,631 - Pf6N2E2_5546 0.16 +1.8 6,360,631 - Pf6N2E2_5546 0.16 +1.2 6,360,631 - Pf6N2E2_5546 0.16 +1.2 6,360,649 - Pf6N2E2_5546 0.18 +1.9
Or see this region's nucleotide sequence