Experiment: L-Lysine 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4769 and Pf6N2E2_4770 are separated by 345 nucleotides Pf6N2E2_4770 and Pf6N2E2_4771 are separated by 127 nucleotides
Pf6N2E2_4769 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 5,508,952 to 5,510,730
_4769
Pf6N2E2_4770 - Stress induced hydrophobic peptide, at 5,511,076 to 5,511,234
_4770
Pf6N2E2_4771 - Coenzyme PQQ synthesis protein F (EC 3.4.99.-), at 5,511,362 to 5,513,191
_4771
Position (kb) 5511
5512 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5510.215 kb on + strand, within Pf6N2E2_4769 at 5510.216 kb on - strand, within Pf6N2E2_4769 at 5510.216 kb on - strand, within Pf6N2E2_4769 at 5510.216 kb on - strand, within Pf6N2E2_4769 at 5510.216 kb on - strand, within Pf6N2E2_4769 at 5510.216 kb on - strand, within Pf6N2E2_4769 at 5510.272 kb on + strand, within Pf6N2E2_4769 at 5510.272 kb on + strand, within Pf6N2E2_4769 at 5510.272 kb on + strand, within Pf6N2E2_4769 at 5510.272 kb on + strand, within Pf6N2E2_4769 at 5510.273 kb on - strand, within Pf6N2E2_4769 at 5510.273 kb on - strand, within Pf6N2E2_4769 at 5510.273 kb on - strand, within Pf6N2E2_4769 at 5510.273 kb on - strand, within Pf6N2E2_4769 at 5510.273 kb on - strand, within Pf6N2E2_4769 at 5510.273 kb on - strand, within Pf6N2E2_4769 at 5510.273 kb on - strand, within Pf6N2E2_4769 at 5510.289 kb on + strand, within Pf6N2E2_4769 at 5510.345 kb on - strand, within Pf6N2E2_4769 at 5510.345 kb on - strand, within Pf6N2E2_4769 at 5510.515 kb on + strand, within Pf6N2E2_4769 at 5510.515 kb on + strand, within Pf6N2E2_4769 at 5510.515 kb on + strand, within Pf6N2E2_4769 at 5510.515 kb on + strand, within Pf6N2E2_4769 at 5510.516 kb on - strand, within Pf6N2E2_4769 at 5510.516 kb on - strand, within Pf6N2E2_4769 at 5510.542 kb on + strand, within Pf6N2E2_4769 at 5510.543 kb on - strand, within Pf6N2E2_4769 at 5510.543 kb on - strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.548 kb on + strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.549 kb on - strand, within Pf6N2E2_4769 at 5510.606 kb on - strand at 5510.635 kb on + strand at 5510.635 kb on + strand at 5510.635 kb on + strand at 5510.635 kb on + strand at 5510.636 kb on - strand at 5510.636 kb on - strand at 5510.659 kb on - strand at 5510.728 kb on + strand at 5510.728 kb on + strand at 5510.728 kb on + strand at 5510.757 kb on + strand at 5510.757 kb on + strand at 5510.806 kb on + strand at 5510.807 kb on - strand at 5510.811 kb on + strand at 5510.811 kb on + strand at 5510.811 kb on + strand at 5510.811 kb on + strand at 5510.811 kb on + strand at 5510.811 kb on + strand at 5510.812 kb on - strand at 5510.812 kb on - strand at 5510.812 kb on - strand at 5510.812 kb on - strand at 5510.812 kb on - strand at 5510.846 kb on + strand at 5510.862 kb on + strand at 5510.862 kb on + strand at 5510.862 kb on + strand at 5510.862 kb on + strand at 5510.862 kb on + strand at 5510.863 kb on - strand at 5510.863 kb on - strand at 5510.863 kb on - strand at 5511.037 kb on + strand at 5511.037 kb on + strand at 5511.037 kb on + strand at 5511.037 kb on + strand at 5511.037 kb on + strand at 5511.037 kb on + strand at 5511.037 kb on + strand at 5511.037 kb on + strand at 5511.037 kb on + strand at 5511.038 kb on - strand at 5511.038 kb on - strand at 5511.038 kb on - strand at 5511.038 kb on - strand at 5511.038 kb on - strand at 5511.038 kb on - strand at 5511.038 kb on - strand at 5511.038 kb on - strand at 5511.038 kb on - strand at 5511.038 kb on - strand at 5511.038 kb on - strand at 5511.039 kb on + strand at 5511.040 kb on - strand at 5511.090 kb on + strand at 5511.090 kb on + strand at 5511.090 kb on + strand at 5511.090 kb on + strand at 5511.091 kb on - strand at 5511.091 kb on - strand at 5511.091 kb on - strand at 5511.091 kb on - strand at 5511.096 kb on + strand, within Pf6N2E2_4770 at 5511.187 kb on + strand, within Pf6N2E2_4770 at 5511.187 kb on + strand, within Pf6N2E2_4770 at 5511.188 kb on - strand, within Pf6N2E2_4770 at 5511.188 kb on - strand, within Pf6N2E2_4770 at 5511.188 kb on - strand, within Pf6N2E2_4770 at 5511.214 kb on + strand, within Pf6N2E2_4770 at 5511.214 kb on + strand, within Pf6N2E2_4770 at 5511.214 kb on + strand, within Pf6N2E2_4770 at 5511.214 kb on + strand, within Pf6N2E2_4770 at 5511.214 kb on + strand, within Pf6N2E2_4770 at 5511.214 kb on + strand, within Pf6N2E2_4770 at 5511.214 kb on + strand, within Pf6N2E2_4770 at 5511.214 kb on + strand, within Pf6N2E2_4770 at 5511.214 kb on + strand, within Pf6N2E2_4770 at 5511.215 kb on - strand, within Pf6N2E2_4770 at 5511.215 kb on - strand, within Pf6N2E2_4770 at 5511.215 kb on - strand, within Pf6N2E2_4770 at 5511.250 kb on + strand at 5511.250 kb on + strand at 5511.250 kb on + strand at 5511.251 kb on - strand at 5511.251 kb on - strand at 5511.365 kb on + strand at 5511.463 kb on - strand at 5511.463 kb on - strand at 5511.465 kb on + strand at 5511.465 kb on + strand at 5511.465 kb on + strand at 5511.465 kb on + strand at 5511.466 kb on - strand at 5511.466 kb on - strand at 5511.466 kb on - strand at 5511.466 kb on - strand at 5511.466 kb on - strand at 5511.497 kb on + strand at 5511.497 kb on + strand at 5511.497 kb on + strand at 5511.498 kb on - strand at 5511.498 kb on - strand at 5511.531 kb on - strand at 5511.661 kb on - strand, within Pf6N2E2_4771 at 5511.661 kb on - strand, within Pf6N2E2_4771 at 5511.717 kb on + strand, within Pf6N2E2_4771 at 5511.717 kb on + strand, within Pf6N2E2_4771 at 5511.718 kb on - strand, within Pf6N2E2_4771 at 5511.718 kb on - strand, within Pf6N2E2_4771 at 5511.772 kb on + strand, within Pf6N2E2_4771 at 5511.772 kb on + strand, within Pf6N2E2_4771 at 5511.772 kb on + strand, within Pf6N2E2_4771 at 5511.772 kb on + strand, within Pf6N2E2_4771 at 5511.772 kb on + strand, within Pf6N2E2_4771 at 5511.772 kb on + strand, within Pf6N2E2_4771 at 5511.772 kb on + strand, within Pf6N2E2_4771 at 5511.772 kb on + strand, within Pf6N2E2_4771 at 5511.772 kb on + strand, within Pf6N2E2_4771 at 5511.772 kb on + strand, within Pf6N2E2_4771 at 5511.772 kb on + strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.773 kb on - strand, within Pf6N2E2_4771 at 5511.774 kb on + strand, within Pf6N2E2_4771 at 5511.774 kb on + strand, within Pf6N2E2_4771 at 5511.775 kb on - strand, within Pf6N2E2_4771 at 5511.840 kb on + strand, within Pf6N2E2_4771 at 5511.840 kb on + strand, within Pf6N2E2_4771 at 5511.841 kb on - strand, within Pf6N2E2_4771 at 5511.841 kb on - strand, within Pf6N2E2_4771 at 5511.907 kb on - strand, within Pf6N2E2_4771 at 5511.919 kb on - strand, within Pf6N2E2_4771 at 5512.189 kb on + strand, within Pf6N2E2_4771 at 5512.189 kb on + strand, within Pf6N2E2_4771 at 5512.189 kb on + strand, within Pf6N2E2_4771 at 5512.189 kb on + strand, within Pf6N2E2_4771
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 25 mM remove 5,510,215 + Pf6N2E2_4769 0.71 +0.4 5,510,216 - Pf6N2E2_4769 0.71 -0.0 5,510,216 - Pf6N2E2_4769 0.71 -0.5 5,510,216 - Pf6N2E2_4769 0.71 +1.0 5,510,216 - Pf6N2E2_4769 0.71 +0.1 5,510,216 - Pf6N2E2_4769 0.71 +0.0 5,510,272 + Pf6N2E2_4769 0.74 +1.2 5,510,272 + Pf6N2E2_4769 0.74 -2.0 5,510,272 + Pf6N2E2_4769 0.74 +0.3 5,510,272 + Pf6N2E2_4769 0.74 -0.3 5,510,273 - Pf6N2E2_4769 0.74 +0.1 5,510,273 - Pf6N2E2_4769 0.74 -0.0 5,510,273 - Pf6N2E2_4769 0.74 +1.1 5,510,273 - Pf6N2E2_4769 0.74 +0.7 5,510,273 - Pf6N2E2_4769 0.74 +0.9 5,510,273 - Pf6N2E2_4769 0.74 +0.7 5,510,273 - Pf6N2E2_4769 0.74 -0.3 5,510,289 + Pf6N2E2_4769 0.75 -0.5 5,510,345 - Pf6N2E2_4769 0.78 -0.5 5,510,345 - Pf6N2E2_4769 0.78 +0.2 5,510,515 + Pf6N2E2_4769 0.88 +0.3 5,510,515 + Pf6N2E2_4769 0.88 -0.9 5,510,515 + Pf6N2E2_4769 0.88 -0.6 5,510,515 + Pf6N2E2_4769 0.88 +0.5 5,510,516 - Pf6N2E2_4769 0.88 -0.7 5,510,516 - Pf6N2E2_4769 0.88 +0.7 5,510,542 + Pf6N2E2_4769 0.89 -0.2 5,510,543 - Pf6N2E2_4769 0.89 +0.6 5,510,543 - Pf6N2E2_4769 0.89 +0.8 5,510,548 + Pf6N2E2_4769 0.90 +0.3 5,510,548 + Pf6N2E2_4769 0.90 +0.0 5,510,548 + Pf6N2E2_4769 0.90 +2.3 5,510,548 + Pf6N2E2_4769 0.90 +0.6 5,510,548 + Pf6N2E2_4769 0.90 -0.0 5,510,548 + Pf6N2E2_4769 0.90 +0.3 5,510,548 + Pf6N2E2_4769 0.90 +0.1 5,510,548 + Pf6N2E2_4769 0.90 -0.5 5,510,548 + Pf6N2E2_4769 0.90 +0.8 5,510,548 + Pf6N2E2_4769 0.90 -1.0 5,510,548 + Pf6N2E2_4769 0.90 -0.3 5,510,548 + Pf6N2E2_4769 0.90 -0.1 5,510,548 + Pf6N2E2_4769 0.90 -0.5 5,510,548 + Pf6N2E2_4769 0.90 -1.2 5,510,548 + Pf6N2E2_4769 0.90 -0.2 5,510,548 + Pf6N2E2_4769 0.90 +0.4 5,510,549 - Pf6N2E2_4769 0.90 +0.4 5,510,549 - +0.7 5,510,549 - Pf6N2E2_4769 0.90 +0.6 5,510,549 - Pf6N2E2_4769 0.90 +0.7 5,510,549 - Pf6N2E2_4769 0.90 -0.4 5,510,549 - Pf6N2E2_4769 0.90 +0.2 5,510,549 - Pf6N2E2_4769 0.90 +0.2 5,510,549 - Pf6N2E2_4769 0.90 +0.1 5,510,549 - Pf6N2E2_4769 0.90 +0.5 5,510,549 - Pf6N2E2_4769 0.90 +0.1 5,510,549 - Pf6N2E2_4769 0.90 +0.7 5,510,549 - Pf6N2E2_4769 0.90 +0.6 5,510,549 - Pf6N2E2_4769 0.90 -0.3 5,510,549 - Pf6N2E2_4769 0.90 +0.9 5,510,606 - -0.1 5,510,635 + +0.6 5,510,635 + +0.9 5,510,635 + +0.5 5,510,635 + +0.2 5,510,636 - +0.8 5,510,636 - -1.4 5,510,659 - +0.1 5,510,728 + -0.6 5,510,728 + +0.0 5,510,728 + -0.2 5,510,757 + +1.1 5,510,757 + +0.2 5,510,806 + -0.4 5,510,807 - -0.5 5,510,811 + -0.1 5,510,811 + -0.0 5,510,811 + -1.0 5,510,811 + -0.0 5,510,811 + -0.5 5,510,811 + -0.1 5,510,812 - +1.1 5,510,812 - +0.5 5,510,812 - +0.1 5,510,812 - +0.0 5,510,812 - -0.8 5,510,846 + +0.3 5,510,862 + -0.2 5,510,862 + -0.1 5,510,862 + +0.6 5,510,862 + +0.6 5,510,862 + -0.4 5,510,863 - -0.0 5,510,863 - +0.7 5,510,863 - +1.5 5,511,037 + +0.4 5,511,037 + -0.6 5,511,037 + +0.7 5,511,037 + -0.3 5,511,037 + +0.4 5,511,037 + -0.2 5,511,037 + +1.0 5,511,037 + +0.3 5,511,037 + +0.1 5,511,038 - -0.2 5,511,038 - +1.2 5,511,038 - +0.9 5,511,038 - +0.3 5,511,038 - -2.2 5,511,038 - +1.1 5,511,038 - -0.0 5,511,038 - -0.5 5,511,038 - -0.5 5,511,038 - -1.2 5,511,038 - +1.0 5,511,039 + -0.7 5,511,040 - -0.2 5,511,090 + +0.1 5,511,090 + +0.4 5,511,090 + -0.1 5,511,090 + +0.1 5,511,091 - -0.2 5,511,091 - +0.6 5,511,091 - -0.5 5,511,091 - -0.2 5,511,096 + Pf6N2E2_4770 0.13 +0.7 5,511,187 + Pf6N2E2_4770 0.70 -2.1 5,511,187 + Pf6N2E2_4770 0.70 +0.3 5,511,188 - Pf6N2E2_4770 0.70 +0.3 5,511,188 - Pf6N2E2_4770 0.70 +0.5 5,511,188 - Pf6N2E2_4770 0.70 +0.5 5,511,214 + Pf6N2E2_4770 0.87 +0.1 5,511,214 + Pf6N2E2_4770 0.87 +0.2 5,511,214 + Pf6N2E2_4770 0.87 +0.7 5,511,214 + Pf6N2E2_4770 0.87 +0.4 5,511,214 + Pf6N2E2_4770 0.87 -1.4 5,511,214 + Pf6N2E2_4770 0.87 +1.5 5,511,214 + Pf6N2E2_4770 0.87 -0.1 5,511,214 + Pf6N2E2_4770 0.87 +1.0 5,511,214 + Pf6N2E2_4770 0.87 +0.7 5,511,215 - Pf6N2E2_4770 0.87 -0.2 5,511,215 - Pf6N2E2_4770 0.87 +1.6 5,511,215 - Pf6N2E2_4770 0.87 +0.7 5,511,250 + +0.1 5,511,250 + -0.0 5,511,250 + +0.4 5,511,251 - -0.8 5,511,251 - -0.6 5,511,365 + -0.4 5,511,463 - -1.7 5,511,463 - -0.0 5,511,465 + +0.0 5,511,465 + +1.1 5,511,465 + +1.1 5,511,465 + -0.1 5,511,466 - +0.0 5,511,466 - -0.3 5,511,466 - -1.1 5,511,466 - -0.5 5,511,466 - -0.2 5,511,497 + -0.8 5,511,497 + +0.0 5,511,497 + +1.1 5,511,498 - +0.5 5,511,498 - -0.1 5,511,531 - -0.7 5,511,661 - Pf6N2E2_4771 0.16 -0.1 5,511,661 - Pf6N2E2_4771 0.16 +0.4 5,511,717 + Pf6N2E2_4771 0.19 +0.5 5,511,717 + Pf6N2E2_4771 0.19 +0.2 5,511,718 - Pf6N2E2_4771 0.19 +0.6 5,511,718 - Pf6N2E2_4771 0.19 +1.0 5,511,772 + Pf6N2E2_4771 0.22 -0.2 5,511,772 + Pf6N2E2_4771 0.22 -0.2 5,511,772 + Pf6N2E2_4771 0.22 -0.9 5,511,772 + Pf6N2E2_4771 0.22 -0.5 5,511,772 + Pf6N2E2_4771 0.22 +0.1 5,511,772 + Pf6N2E2_4771 0.22 -0.2 5,511,772 + Pf6N2E2_4771 0.22 +0.7 5,511,772 + Pf6N2E2_4771 0.22 +1.1 5,511,772 + Pf6N2E2_4771 0.22 -0.9 5,511,772 + Pf6N2E2_4771 0.22 +1.0 5,511,772 + Pf6N2E2_4771 0.22 -0.3 5,511,773 - Pf6N2E2_4771 0.22 -0.4 5,511,773 - Pf6N2E2_4771 0.22 -0.2 5,511,773 - Pf6N2E2_4771 0.22 +0.6 5,511,773 - Pf6N2E2_4771 0.22 +0.2 5,511,773 - Pf6N2E2_4771 0.22 -0.7 5,511,773 - Pf6N2E2_4771 0.22 -0.4 5,511,773 - Pf6N2E2_4771 0.22 -1.0 5,511,773 - Pf6N2E2_4771 0.22 +1.5 5,511,773 - Pf6N2E2_4771 0.22 +0.8 5,511,773 - Pf6N2E2_4771 0.22 -0.1 5,511,773 - Pf6N2E2_4771 0.22 +0.1 5,511,773 - Pf6N2E2_4771 0.22 +0.2 5,511,773 - Pf6N2E2_4771 0.22 -0.1 5,511,773 - Pf6N2E2_4771 0.22 +1.1 5,511,774 + Pf6N2E2_4771 0.23 +0.3 5,511,774 + Pf6N2E2_4771 0.23 +0.5 5,511,775 - Pf6N2E2_4771 0.23 -2.5 5,511,840 + Pf6N2E2_4771 0.26 +0.2 5,511,840 + Pf6N2E2_4771 0.26 -0.4 5,511,841 - Pf6N2E2_4771 0.26 +0.6 5,511,841 - Pf6N2E2_4771 0.26 -0.1 5,511,907 - Pf6N2E2_4771 0.30 -0.6 5,511,919 - Pf6N2E2_4771 0.30 +1.0 5,512,189 + Pf6N2E2_4771 0.45 -0.4 5,512,189 + Pf6N2E2_4771 0.45 +0.3 5,512,189 + Pf6N2E2_4771 0.45 -0.3 5,512,189 + Pf6N2E2_4771 0.45 -0.3
Or see this region's nucleotide sequence