Experiment: L-Lysine 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4720 and Pf6N2E2_4721 are separated by 135 nucleotides Pf6N2E2_4721 and Pf6N2E2_4722 are separated by 221 nucleotides
Pf6N2E2_4720 - Sarcosine oxidase alpha subunit (EC 1.5.3.1), at 5,456,786 to 5,459,803
_4720
Pf6N2E2_4721 - Sarcosine oxidase gamma subunit (EC 1.5.3.1), at 5,459,939 to 5,460,571
_4721
Pf6N2E2_4722 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 5,460,793 to 5,461,650
_4722
Position (kb)
5459
5460
5461 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5459.027 kb on + strand, within Pf6N2E2_4720 at 5459.028 kb on - strand, within Pf6N2E2_4720 at 5459.028 kb on - strand, within Pf6N2E2_4720 at 5459.028 kb on - strand, within Pf6N2E2_4720 at 5459.060 kb on + strand, within Pf6N2E2_4720 at 5459.060 kb on + strand, within Pf6N2E2_4720 at 5459.060 kb on + strand, within Pf6N2E2_4720 at 5459.060 kb on + strand, within Pf6N2E2_4720 at 5459.060 kb on + strand, within Pf6N2E2_4720 at 5459.061 kb on - strand, within Pf6N2E2_4720 at 5459.061 kb on - strand, within Pf6N2E2_4720 at 5459.249 kb on + strand, within Pf6N2E2_4720 at 5459.249 kb on + strand, within Pf6N2E2_4720 at 5459.273 kb on + strand, within Pf6N2E2_4720 at 5459.318 kb on + strand, within Pf6N2E2_4720 at 5459.318 kb on + strand, within Pf6N2E2_4720 at 5459.318 kb on + strand, within Pf6N2E2_4720 at 5459.319 kb on - strand, within Pf6N2E2_4720 at 5459.319 kb on - strand, within Pf6N2E2_4720 at 5459.334 kb on - strand, within Pf6N2E2_4720 at 5459.355 kb on + strand, within Pf6N2E2_4720 at 5459.355 kb on + strand, within Pf6N2E2_4720 at 5459.355 kb on + strand, within Pf6N2E2_4720 at 5459.355 kb on + strand, within Pf6N2E2_4720 at 5459.355 kb on + strand, within Pf6N2E2_4720 at 5459.355 kb on + strand, within Pf6N2E2_4720 at 5459.355 kb on + strand, within Pf6N2E2_4720 at 5459.356 kb on - strand, within Pf6N2E2_4720 at 5459.356 kb on - strand, within Pf6N2E2_4720 at 5459.356 kb on - strand, within Pf6N2E2_4720 at 5459.356 kb on - strand, within Pf6N2E2_4720 at 5459.442 kb on + strand, within Pf6N2E2_4720 at 5459.636 kb on + strand at 5459.636 kb on + strand at 5459.636 kb on + strand at 5459.636 kb on + strand at 5459.636 kb on + strand at 5459.637 kb on - strand at 5459.637 kb on - strand at 5459.797 kb on + strand at 5459.920 kb on + strand at 5459.957 kb on + strand at 5459.957 kb on + strand at 5459.958 kb on - strand at 5459.958 kb on - strand at 5460.384 kb on - strand, within Pf6N2E2_4721 at 5460.384 kb on - strand, within Pf6N2E2_4721 at 5460.384 kb on - strand, within Pf6N2E2_4721 at 5460.384 kb on - strand, within Pf6N2E2_4721 at 5460.384 kb on - strand, within Pf6N2E2_4721 at 5460.384 kb on - strand, within Pf6N2E2_4721 at 5460.384 kb on - strand, within Pf6N2E2_4721 at 5460.582 kb on + strand at 5460.583 kb on - strand at 5460.613 kb on + strand at 5460.674 kb on + strand at 5460.675 kb on - strand at 5460.728 kb on - strand at 5460.728 kb on - strand at 5460.772 kb on + strand at 5460.850 kb on - strand at 5460.892 kb on + strand, within Pf6N2E2_4722 at 5461.026 kb on + strand, within Pf6N2E2_4722 at 5461.213 kb on + strand, within Pf6N2E2_4722 at 5461.214 kb on - strand, within Pf6N2E2_4722 at 5461.214 kb on - strand, within Pf6N2E2_4722 at 5461.214 kb on - strand, within Pf6N2E2_4722 at 5461.214 kb on - strand, within Pf6N2E2_4722 at 5461.312 kb on + strand, within Pf6N2E2_4722 at 5461.423 kb on + strand, within Pf6N2E2_4722 at 5461.423 kb on + strand, within Pf6N2E2_4722 at 5461.423 kb on + strand, within Pf6N2E2_4722 at 5461.462 kb on + strand, within Pf6N2E2_4722 at 5461.462 kb on + strand, within Pf6N2E2_4722 at 5461.462 kb on + strand, within Pf6N2E2_4722 at 5461.462 kb on + strand, within Pf6N2E2_4722 at 5461.462 kb on + strand, within Pf6N2E2_4722 at 5461.462 kb on + strand, within Pf6N2E2_4722 at 5461.463 kb on - strand, within Pf6N2E2_4722 at 5461.463 kb on - strand, within Pf6N2E2_4722 at 5461.463 kb on - strand, within Pf6N2E2_4722 at 5461.531 kb on + strand, within Pf6N2E2_4722
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 25 mM remove 5,459,027 + Pf6N2E2_4720 0.74 +0.4 5,459,028 - Pf6N2E2_4720 0.74 +0.2 5,459,028 - Pf6N2E2_4720 0.74 -1.0 5,459,028 - Pf6N2E2_4720 0.74 +0.1 5,459,060 + Pf6N2E2_4720 0.75 -0.9 5,459,060 + Pf6N2E2_4720 0.75 +0.8 5,459,060 + Pf6N2E2_4720 0.75 -1.3 5,459,060 + Pf6N2E2_4720 0.75 -0.2 5,459,060 + Pf6N2E2_4720 0.75 -1.0 5,459,061 - Pf6N2E2_4720 0.75 -0.8 5,459,061 - Pf6N2E2_4720 0.75 -0.4 5,459,249 + Pf6N2E2_4720 0.82 -0.5 5,459,249 + Pf6N2E2_4720 0.82 -0.8 5,459,273 + Pf6N2E2_4720 0.82 +1.4 5,459,318 + Pf6N2E2_4720 0.84 +0.5 5,459,318 + Pf6N2E2_4720 0.84 +0.2 5,459,318 + Pf6N2E2_4720 0.84 -0.1 5,459,319 - Pf6N2E2_4720 0.84 +0.4 5,459,319 - Pf6N2E2_4720 0.84 +0.1 5,459,334 - Pf6N2E2_4720 0.84 +0.5 5,459,355 + Pf6N2E2_4720 0.85 +0.1 5,459,355 + Pf6N2E2_4720 0.85 -0.2 5,459,355 + Pf6N2E2_4720 0.85 +0.2 5,459,355 + Pf6N2E2_4720 0.85 -0.1 5,459,355 + Pf6N2E2_4720 0.85 +0.6 5,459,355 + Pf6N2E2_4720 0.85 +0.5 5,459,355 + Pf6N2E2_4720 0.85 +0.2 5,459,356 - Pf6N2E2_4720 0.85 +0.2 5,459,356 - Pf6N2E2_4720 0.85 +0.3 5,459,356 - Pf6N2E2_4720 0.85 -0.0 5,459,356 - Pf6N2E2_4720 0.85 -0.7 5,459,442 + Pf6N2E2_4720 0.88 -0.8 5,459,636 + +0.2 5,459,636 + -0.1 5,459,636 + +0.5 5,459,636 + +0.3 5,459,636 + -0.3 5,459,637 - -0.2 5,459,637 - -0.3 5,459,797 + -0.2 5,459,920 + -0.4 5,459,957 + -0.5 5,459,957 + +0.8 5,459,958 - +0.3 5,459,958 - +0.5 5,460,384 - Pf6N2E2_4721 0.70 +0.4 5,460,384 - Pf6N2E2_4721 0.70 +0.2 5,460,384 - Pf6N2E2_4721 0.70 +0.9 5,460,384 - Pf6N2E2_4721 0.70 -0.2 5,460,384 - Pf6N2E2_4721 0.70 +0.6 5,460,384 - Pf6N2E2_4721 0.70 +1.9 5,460,384 - Pf6N2E2_4721 0.70 +0.6 5,460,582 + +0.9 5,460,583 - +1.6 5,460,613 + +0.5 5,460,674 + +0.3 5,460,675 - +0.6 5,460,728 - +0.2 5,460,728 - -0.5 5,460,772 + +0.5 5,460,850 - +0.0 5,460,892 + Pf6N2E2_4722 0.12 +0.6 5,461,026 + Pf6N2E2_4722 0.27 +1.3 5,461,213 + Pf6N2E2_4722 0.49 -0.3 5,461,214 - Pf6N2E2_4722 0.49 +1.3 5,461,214 - Pf6N2E2_4722 0.49 +0.4 5,461,214 - Pf6N2E2_4722 0.49 +0.1 5,461,214 - Pf6N2E2_4722 0.49 -0.3 5,461,312 + Pf6N2E2_4722 0.60 +0.4 5,461,423 + Pf6N2E2_4722 0.73 -0.3 5,461,423 + Pf6N2E2_4722 0.73 +0.2 5,461,423 + Pf6N2E2_4722 0.73 -0.9 5,461,462 + Pf6N2E2_4722 0.78 +0.3 5,461,462 + Pf6N2E2_4722 0.78 +0.3 5,461,462 + Pf6N2E2_4722 0.78 +0.0 5,461,462 + Pf6N2E2_4722 0.78 +0.3 5,461,462 + Pf6N2E2_4722 0.78 +0.2 5,461,462 + Pf6N2E2_4722 0.78 -2.4 5,461,463 - Pf6N2E2_4722 0.78 -0.6 5,461,463 - Pf6N2E2_4722 0.78 +0.2 5,461,463 - Pf6N2E2_4722 0.78 -0.1 5,461,531 + Pf6N2E2_4722 0.86 +1.2
Or see this region's nucleotide sequence