Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4432

Experiment: L-Lysine 25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4431 and Pf6N2E2_4432 overlap by 4 nucleotidesPf6N2E2_4432 and Pf6N2E2_4433 overlap by 4 nucleotides Pf6N2E2_4431 - Porphobilinogen deaminase (EC 2.5.1.61), at 5,162,355 to 5,163,296 _4431 Pf6N2E2_4432 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 5,163,293 to 5,165,209 _4432 Pf6N2E2_4433 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 5,165,206 to 5,166,453 _4433 Position (kb) 5163 5164 5165 5166Strain fitness (log2 ratio) -3 -2 -1 0 1at 5162.319 kb on + strandat 5162.319 kb on + strandat 5162.331 kb on + strandat 5162.338 kb on + strandat 5162.342 kb on + strandat 5163.273 kb on + strandat 5164.057 kb on + strand, within Pf6N2E2_4432at 5164.058 kb on - strand, within Pf6N2E2_4432at 5164.073 kb on + strand, within Pf6N2E2_4432at 5164.073 kb on + strand, within Pf6N2E2_4432at 5164.073 kb on + strand, within Pf6N2E2_4432at 5164.073 kb on + strand, within Pf6N2E2_4432at 5164.073 kb on + strand, within Pf6N2E2_4432at 5164.073 kb on + strand, within Pf6N2E2_4432at 5164.074 kb on - strand, within Pf6N2E2_4432at 5164.074 kb on - strand, within Pf6N2E2_4432at 5164.107 kb on + strand, within Pf6N2E2_4432at 5164.107 kb on + strand, within Pf6N2E2_4432at 5164.107 kb on + strand, within Pf6N2E2_4432at 5164.107 kb on + strand, within Pf6N2E2_4432at 5164.108 kb on - strand, within Pf6N2E2_4432at 5164.108 kb on - strand, within Pf6N2E2_4432at 5164.108 kb on - strand, within Pf6N2E2_4432at 5164.182 kb on + strand, within Pf6N2E2_4432at 5164.182 kb on + strand, within Pf6N2E2_4432at 5164.182 kb on + strand, within Pf6N2E2_4432at 5164.182 kb on + strand, within Pf6N2E2_4432at 5164.183 kb on - strand, within Pf6N2E2_4432at 5164.183 kb on - strand, within Pf6N2E2_4432at 5164.183 kb on - strand, within Pf6N2E2_4432at 5164.183 kb on - strand, within Pf6N2E2_4432at 5164.183 kb on - strand, within Pf6N2E2_4432at 5164.183 kb on - strand, within Pf6N2E2_4432at 5164.183 kb on - strand, within Pf6N2E2_4432at 5164.262 kb on - strand, within Pf6N2E2_4432at 5164.739 kb on + strand, within Pf6N2E2_4432at 5164.739 kb on + strand, within Pf6N2E2_4432at 5164.740 kb on - strand, within Pf6N2E2_4432at 5164.740 kb on - strand, within Pf6N2E2_4432at 5164.830 kb on - strand, within Pf6N2E2_4432at 5164.831 kb on + strand, within Pf6N2E2_4432at 5164.968 kb on - strand, within Pf6N2E2_4432at 5165.129 kb on + strandat 5165.130 kb on - strandat 5165.130 kb on - strandat 5165.218 kb on + strandat 5165.218 kb on + strandat 5165.219 kb on - strandat 5165.287 kb on + strandat 5165.288 kb on - strandat 5165.302 kb on + strandat 5165.406 kb on + strand, within Pf6N2E2_4433at 5165.406 kb on + strand, within Pf6N2E2_4433at 5165.406 kb on + strand, within Pf6N2E2_4433at 5165.407 kb on - strand, within Pf6N2E2_4433at 5165.407 kb on - strand, within Pf6N2E2_4433at 5165.566 kb on + strand, within Pf6N2E2_4433at 5165.566 kb on + strand, within Pf6N2E2_4433at 5165.566 kb on + strand, within Pf6N2E2_4433at 5165.566 kb on + strand, within Pf6N2E2_4433at 5165.567 kb on - strand, within Pf6N2E2_4433at 5165.567 kb on - strand, within Pf6N2E2_4433at 5165.567 kb on - strand, within Pf6N2E2_4433at 5165.567 kb on - strand, within Pf6N2E2_4433at 5165.569 kb on + strand, within Pf6N2E2_4433at 5165.570 kb on - strand, within Pf6N2E2_4433at 5165.608 kb on + strand, within Pf6N2E2_4433at 5165.608 kb on + strand, within Pf6N2E2_4433at 5165.609 kb on - strand, within Pf6N2E2_4433at 5165.609 kb on - strand, within Pf6N2E2_4433at 5166.028 kb on + strand, within Pf6N2E2_4433at 5166.028 kb on + strand, within Pf6N2E2_4433at 5166.028 kb on + strand, within Pf6N2E2_4433at 5166.029 kb on - strand, within Pf6N2E2_4433at 5166.077 kb on + strand, within Pf6N2E2_4433at 5166.077 kb on + strand, within Pf6N2E2_4433at 5166.078 kb on - strand, within Pf6N2E2_4433at 5166.103 kb on + strand, within Pf6N2E2_4433at 5166.103 kb on + strand, within Pf6N2E2_4433at 5166.104 kb on - strand, within Pf6N2E2_4433at 5166.127 kb on + strand, within Pf6N2E2_4433

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lysine 25 mM
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5,162,319 + -0.1
5,162,319 + -0.5
5,162,331 + +0.0
5,162,338 + -0.1
5,162,342 + +0.9
5,163,273 + -1.5
5,164,057 + Pf6N2E2_4432 0.40 -0.5
5,164,058 - Pf6N2E2_4432 0.40 -0.3
5,164,073 + Pf6N2E2_4432 0.41 +0.4
5,164,073 + Pf6N2E2_4432 0.41 +0.3
5,164,073 + Pf6N2E2_4432 0.41 -0.8
5,164,073 + Pf6N2E2_4432 0.41 -1.0
5,164,073 + Pf6N2E2_4432 0.41 +0.4
5,164,073 + Pf6N2E2_4432 0.41 -0.0
5,164,074 - Pf6N2E2_4432 0.41 -0.6
5,164,074 - Pf6N2E2_4432 0.41 -0.1
5,164,107 + Pf6N2E2_4432 0.42 -1.3
5,164,107 + Pf6N2E2_4432 0.42 -0.7
5,164,107 + Pf6N2E2_4432 0.42 -1.1
5,164,107 + Pf6N2E2_4432 0.42 +0.1
5,164,108 - Pf6N2E2_4432 0.43 +0.0
5,164,108 - Pf6N2E2_4432 0.43 -1.2
5,164,108 - Pf6N2E2_4432 0.43 -0.5
5,164,182 + Pf6N2E2_4432 0.46 -0.6
5,164,182 + Pf6N2E2_4432 0.46 +1.3
5,164,182 + Pf6N2E2_4432 0.46 -0.3
5,164,182 + Pf6N2E2_4432 0.46 -0.4
5,164,183 - Pf6N2E2_4432 0.46 -1.5
5,164,183 - Pf6N2E2_4432 0.46 -3.0
5,164,183 - Pf6N2E2_4432 0.46 +0.1
5,164,183 - Pf6N2E2_4432 0.46 -2.0
5,164,183 - Pf6N2E2_4432 0.46 -1.3
5,164,183 - Pf6N2E2_4432 0.46 -1.8
5,164,183 - Pf6N2E2_4432 0.46 -2.0
5,164,262 - Pf6N2E2_4432 0.51 -1.0
5,164,739 + Pf6N2E2_4432 0.75 -0.4
5,164,739 + Pf6N2E2_4432 0.75 -0.8
5,164,740 - Pf6N2E2_4432 0.75 -0.2
5,164,740 - Pf6N2E2_4432 0.75 -0.9
5,164,830 - Pf6N2E2_4432 0.80 +0.1
5,164,831 + Pf6N2E2_4432 0.80 -1.1
5,164,968 - Pf6N2E2_4432 0.87 -0.6
5,165,129 + -0.2
5,165,130 - -1.3
5,165,130 - -0.5
5,165,218 + +0.2
5,165,218 + -0.4
5,165,219 - -1.5
5,165,287 + -1.3
5,165,288 - -1.6
5,165,302 + -1.8
5,165,406 + Pf6N2E2_4433 0.16 -0.5
5,165,406 + Pf6N2E2_4433 0.16 +0.5
5,165,406 + Pf6N2E2_4433 0.16 +0.2
5,165,407 - Pf6N2E2_4433 0.16 -0.7
5,165,407 - Pf6N2E2_4433 0.16 -2.5
5,165,566 + Pf6N2E2_4433 0.29 -0.7
5,165,566 + Pf6N2E2_4433 0.29 +0.0
5,165,566 + Pf6N2E2_4433 0.29 -0.3
5,165,566 + Pf6N2E2_4433 0.29 +0.1
5,165,567 - Pf6N2E2_4433 0.29 -0.1
5,165,567 - Pf6N2E2_4433 0.29 -1.8
5,165,567 - Pf6N2E2_4433 0.29 -0.1
5,165,567 - Pf6N2E2_4433 0.29 -0.7
5,165,569 + Pf6N2E2_4433 0.29 -0.6
5,165,570 - Pf6N2E2_4433 0.29 -0.2
5,165,608 + Pf6N2E2_4433 0.32 -1.0
5,165,608 + Pf6N2E2_4433 0.32 -2.0
5,165,609 - Pf6N2E2_4433 0.32 +0.1
5,165,609 - Pf6N2E2_4433 0.32 -1.2
5,166,028 + Pf6N2E2_4433 0.66 -1.3
5,166,028 + Pf6N2E2_4433 0.66 -1.8
5,166,028 + Pf6N2E2_4433 0.66 -0.5
5,166,029 - Pf6N2E2_4433 0.66 -1.1
5,166,077 + Pf6N2E2_4433 0.70 -0.6
5,166,077 + Pf6N2E2_4433 0.70 -0.2
5,166,078 - Pf6N2E2_4433 0.70 -0.9
5,166,103 + Pf6N2E2_4433 0.72 -0.8
5,166,103 + Pf6N2E2_4433 0.72 +0.7
5,166,104 - Pf6N2E2_4433 0.72 +0.4
5,166,127 + Pf6N2E2_4433 0.74 -0.3

Or see this region's nucleotide sequence