Experiment: L-Lysine 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4413 and Pf6N2E2_4414 are separated by 102 nucleotides Pf6N2E2_4414 and Pf6N2E2_4415 are separated by 207 nucleotides Pf6N2E2_4415 and Pf6N2E2_4416 overlap by 4 nucleotides Pf6N2E2_4416 and Pf6N2E2_4417 are separated by 2 nucleotides
Pf6N2E2_4413 - FIG004064: hypothetical protein, at 5,147,840 to 5,148,265
_4413
Pf6N2E2_4414 - FIG00953808: hypothetical protein, at 5,148,368 to 5,148,691
_4414
Pf6N2E2_4415 - Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase, at 5,148,899 to 5,149,603
_4415
Pf6N2E2_4416 - Tyrosine recombinase XerC, at 5,149,600 to 5,150,499
_4416
Pf6N2E2_4417 - Protein of unknown function DUF484, at 5,150,502 to 5,151,224
_4417
Position (kb)
5148
5149
5150 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5147.971 kb on + strand, within Pf6N2E2_4413 at 5147.971 kb on + strand, within Pf6N2E2_4413 at 5147.971 kb on + strand, within Pf6N2E2_4413 at 5147.971 kb on + strand, within Pf6N2E2_4413 at 5147.971 kb on + strand, within Pf6N2E2_4413 at 5147.971 kb on + strand, within Pf6N2E2_4413 at 5147.972 kb on - strand, within Pf6N2E2_4413 at 5147.972 kb on - strand, within Pf6N2E2_4413 at 5147.972 kb on - strand, within Pf6N2E2_4413 at 5148.015 kb on + strand, within Pf6N2E2_4413 at 5148.015 kb on + strand, within Pf6N2E2_4413 at 5148.015 kb on + strand, within Pf6N2E2_4413 at 5148.015 kb on + strand, within Pf6N2E2_4413 at 5148.015 kb on + strand, within Pf6N2E2_4413 at 5148.015 kb on + strand, within Pf6N2E2_4413 at 5148.071 kb on + strand, within Pf6N2E2_4413 at 5148.071 kb on + strand, within Pf6N2E2_4413 at 5148.072 kb on - strand, within Pf6N2E2_4413 at 5148.194 kb on + strand, within Pf6N2E2_4413 at 5148.195 kb on - strand, within Pf6N2E2_4413 at 5148.248 kb on + strand at 5148.249 kb on - strand at 5148.249 kb on - strand at 5148.249 kb on - strand at 5148.263 kb on + strand at 5148.330 kb on + strand at 5148.332 kb on + strand at 5148.332 kb on + strand at 5148.332 kb on + strand at 5148.332 kb on + strand at 5148.332 kb on + strand at 5148.333 kb on - strand at 5148.333 kb on - strand at 5148.336 kb on + strand at 5148.336 kb on + strand at 5148.336 kb on + strand at 5148.336 kb on + strand at 5148.336 kb on + strand at 5148.336 kb on + strand at 5148.336 kb on + strand at 5148.336 kb on + strand at 5148.337 kb on - strand at 5148.337 kb on - strand at 5148.362 kb on + strand at 5148.363 kb on - strand at 5148.363 kb on - strand at 5148.405 kb on + strand, within Pf6N2E2_4414 at 5148.405 kb on + strand, within Pf6N2E2_4414 at 5148.406 kb on - strand, within Pf6N2E2_4414 at 5148.406 kb on - strand, within Pf6N2E2_4414 at 5148.543 kb on + strand, within Pf6N2E2_4414 at 5148.671 kb on + strand at 5148.671 kb on + strand at 5148.671 kb on + strand at 5148.671 kb on + strand at 5148.671 kb on + strand at 5148.685 kb on + strand at 5148.685 kb on + strand at 5148.686 kb on - strand at 5148.693 kb on + strand at 5148.837 kb on + strand at 5148.837 kb on + strand at 5148.837 kb on + strand at 5148.837 kb on + strand at 5148.838 kb on - strand at 5148.927 kb on - strand at 5149.180 kb on - strand, within Pf6N2E2_4415 at 5149.180 kb on - strand, within Pf6N2E2_4415 at 5149.523 kb on + strand, within Pf6N2E2_4415 at 5149.523 kb on + strand, within Pf6N2E2_4415 at 5149.523 kb on + strand, within Pf6N2E2_4415 at 5149.685 kb on + strand at 5149.720 kb on + strand, within Pf6N2E2_4416 at 5149.721 kb on - strand, within Pf6N2E2_4416 at 5150.040 kb on + strand, within Pf6N2E2_4416 at 5150.078 kb on + strand, within Pf6N2E2_4416 at 5150.079 kb on - strand, within Pf6N2E2_4416 at 5150.079 kb on - strand, within Pf6N2E2_4416 at 5150.253 kb on - strand, within Pf6N2E2_4416 at 5150.259 kb on - strand, within Pf6N2E2_4416 at 5150.509 kb on - strand at 5150.566 kb on + strand at 5150.566 kb on + strand at 5150.567 kb on - strand at 5150.599 kb on + strand, within Pf6N2E2_4417 at 5150.599 kb on + strand, within Pf6N2E2_4417 at 5150.600 kb on - strand, within Pf6N2E2_4417 at 5150.600 kb on - strand, within Pf6N2E2_4417 at 5150.600 kb on - strand, within Pf6N2E2_4417
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 25 mM remove 5,147,971 + Pf6N2E2_4413 0.31 -2.3 5,147,971 + Pf6N2E2_4413 0.31 -0.8 5,147,971 + Pf6N2E2_4413 0.31 -0.1 5,147,971 + Pf6N2E2_4413 0.31 -0.4 5,147,971 + Pf6N2E2_4413 0.31 -0.6 5,147,971 + Pf6N2E2_4413 0.31 -0.3 5,147,972 - Pf6N2E2_4413 0.31 +1.2 5,147,972 - Pf6N2E2_4413 0.31 +1.0 5,147,972 - Pf6N2E2_4413 0.31 +0.6 5,148,015 + Pf6N2E2_4413 0.41 -1.4 5,148,015 + Pf6N2E2_4413 0.41 -0.6 5,148,015 + Pf6N2E2_4413 0.41 -0.8 5,148,015 + Pf6N2E2_4413 0.41 -0.1 5,148,015 + Pf6N2E2_4413 0.41 -0.9 5,148,015 + Pf6N2E2_4413 0.41 -0.3 5,148,071 + Pf6N2E2_4413 0.54 -1.0 5,148,071 + Pf6N2E2_4413 0.54 -0.2 5,148,072 - Pf6N2E2_4413 0.54 +1.2 5,148,194 + Pf6N2E2_4413 0.83 -2.4 5,148,195 - Pf6N2E2_4413 0.83 +0.9 5,148,248 + +0.4 5,148,249 - +1.1 5,148,249 - +1.1 5,148,249 - +2.4 5,148,263 + -0.7 5,148,330 + -1.5 5,148,332 + -0.0 5,148,332 + -0.8 5,148,332 + -0.2 5,148,332 + -0.4 5,148,332 + +0.3 5,148,333 - +0.7 5,148,333 - -0.3 5,148,336 + -0.5 5,148,336 + -0.9 5,148,336 + -1.1 5,148,336 + -0.5 5,148,336 + -2.5 5,148,336 + -0.3 5,148,336 + -0.9 5,148,336 + -2.2 5,148,337 - +0.7 5,148,337 - +1.5 5,148,362 + +1.0 5,148,363 - +0.8 5,148,363 - +1.0 5,148,405 + Pf6N2E2_4414 0.11 -0.3 5,148,405 + Pf6N2E2_4414 0.11 +0.6 5,148,406 - Pf6N2E2_4414 0.12 +0.9 5,148,406 - Pf6N2E2_4414 0.12 +1.0 5,148,543 + Pf6N2E2_4414 0.54 +1.9 5,148,671 + +0.8 5,148,671 + -0.0 5,148,671 + +0.7 5,148,671 + +0.6 5,148,671 + -0.1 5,148,685 + +0.8 5,148,685 + +0.9 5,148,686 - -1.3 5,148,693 + +1.1 5,148,837 + +0.1 5,148,837 + +0.4 5,148,837 + -1.0 5,148,837 + +0.8 5,148,838 - +0.0 5,148,927 - +0.7 5,149,180 - Pf6N2E2_4415 0.40 -0.9 5,149,180 - Pf6N2E2_4415 0.40 +0.9 5,149,523 + Pf6N2E2_4415 0.89 +0.5 5,149,523 + Pf6N2E2_4415 0.89 -0.3 5,149,523 + Pf6N2E2_4415 0.89 -1.1 5,149,685 + -2.0 5,149,720 + Pf6N2E2_4416 0.13 -0.6 5,149,721 - Pf6N2E2_4416 0.13 +0.4 5,150,040 + Pf6N2E2_4416 0.49 -2.2 5,150,078 + Pf6N2E2_4416 0.53 -1.3 5,150,079 - Pf6N2E2_4416 0.53 -1.6 5,150,079 - Pf6N2E2_4416 0.53 -1.9 5,150,253 - Pf6N2E2_4416 0.73 -1.2 5,150,259 - Pf6N2E2_4416 0.73 +0.6 5,150,509 - +1.7 5,150,566 + -0.3 5,150,566 + -1.1 5,150,567 - +0.1 5,150,599 + Pf6N2E2_4417 0.13 +0.4 5,150,599 + Pf6N2E2_4417 0.13 -0.9 5,150,600 - Pf6N2E2_4417 0.14 -0.5 5,150,600 - Pf6N2E2_4417 0.14 -0.1 5,150,600 - Pf6N2E2_4417 0.14 -0.3
Or see this region's nucleotide sequence