Experiment: L-Lysine 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3385 and Pf6N2E2_3386 are separated by 127 nucleotides Pf6N2E2_3386 and Pf6N2E2_3387 are separated by 674 nucleotides
Pf6N2E2_3385 - Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing, at 3,915,154 to 3,917,964
_3385
Pf6N2E2_3386 - hypothetical protein, at 3,918,092 to 3,918,370
_3386
Pf6N2E2_3387 - Phage integrase, at 3,919,045 to 3,920,301
_3387
Position (kb)
3918
3919 Strain fitness (log2 ratio)
-1
0
1
2 at 3917.121 kb on - strand, within Pf6N2E2_3385 at 3917.266 kb on + strand, within Pf6N2E2_3385 at 3917.266 kb on + strand, within Pf6N2E2_3385 at 3917.266 kb on + strand, within Pf6N2E2_3385 at 3917.267 kb on - strand, within Pf6N2E2_3385 at 3917.313 kb on + strand, within Pf6N2E2_3385 at 3917.314 kb on - strand, within Pf6N2E2_3385 at 3917.515 kb on + strand, within Pf6N2E2_3385 at 3917.515 kb on + strand, within Pf6N2E2_3385 at 3917.516 kb on - strand, within Pf6N2E2_3385 at 3917.581 kb on + strand, within Pf6N2E2_3385 at 3917.581 kb on + strand, within Pf6N2E2_3385 at 3917.581 kb on + strand, within Pf6N2E2_3385 at 3917.581 kb on + strand, within Pf6N2E2_3385 at 3917.728 kb on - strand at 3917.837 kb on + strand at 3917.837 kb on + strand at 3917.890 kb on - strand at 3917.926 kb on + strand at 3917.926 kb on + strand at 3917.927 kb on - strand at 3917.927 kb on - strand at 3917.927 kb on - strand at 3917.927 kb on - strand at 3917.972 kb on + strand at 3917.972 kb on + strand at 3917.972 kb on + strand at 3917.973 kb on - strand at 3917.973 kb on - strand at 3918.105 kb on + strand at 3918.105 kb on + strand at 3918.105 kb on + strand at 3918.106 kb on - strand at 3918.106 kb on - strand at 3918.106 kb on - strand at 3918.106 kb on - strand at 3918.111 kb on + strand at 3918.111 kb on + strand at 3918.112 kb on - strand at 3918.112 kb on - strand at 3918.172 kb on + strand, within Pf6N2E2_3386 at 3918.173 kb on - strand, within Pf6N2E2_3386 at 3918.173 kb on - strand, within Pf6N2E2_3386 at 3918.173 kb on - strand, within Pf6N2E2_3386 at 3918.210 kb on - strand, within Pf6N2E2_3386 at 3918.210 kb on - strand, within Pf6N2E2_3386 at 3918.210 kb on - strand, within Pf6N2E2_3386 at 3918.232 kb on + strand, within Pf6N2E2_3386 at 3918.233 kb on - strand, within Pf6N2E2_3386 at 3918.358 kb on + strand at 3918.358 kb on + strand at 3918.358 kb on + strand at 3918.359 kb on - strand at 3918.871 kb on - strand at 3918.997 kb on + strand at 3919.050 kb on + strand at 3919.050 kb on + strand at 3919.051 kb on - strand at 3919.105 kb on - strand at 3919.105 kb on - strand at 3919.105 kb on - strand at 3919.105 kb on - strand at 3919.119 kb on + strand at 3919.328 kb on + strand, within Pf6N2E2_3387 at 3919.328 kb on + strand, within Pf6N2E2_3387 at 3919.328 kb on + strand, within Pf6N2E2_3387 at 3919.329 kb on - strand, within Pf6N2E2_3387 at 3919.329 kb on - strand, within Pf6N2E2_3387 at 3919.329 kb on - strand, within Pf6N2E2_3387
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 25 mM remove 3,917,121 - Pf6N2E2_3385 0.70 -1.0 3,917,266 + Pf6N2E2_3385 0.75 -0.3 3,917,266 + Pf6N2E2_3385 0.75 +1.0 3,917,266 + Pf6N2E2_3385 0.75 -1.0 3,917,267 - Pf6N2E2_3385 0.75 +0.0 3,917,313 + Pf6N2E2_3385 0.77 -0.2 3,917,314 - Pf6N2E2_3385 0.77 +1.0 3,917,515 + Pf6N2E2_3385 0.84 +0.2 3,917,515 + Pf6N2E2_3385 0.84 -0.2 3,917,516 - Pf6N2E2_3385 0.84 +0.1 3,917,581 + Pf6N2E2_3385 0.86 -0.4 3,917,581 + Pf6N2E2_3385 0.86 +0.5 3,917,581 + Pf6N2E2_3385 0.86 +0.9 3,917,581 + Pf6N2E2_3385 0.86 +0.0 3,917,728 - +0.3 3,917,837 + +0.3 3,917,837 + +0.2 3,917,890 - -0.1 3,917,926 + -1.6 3,917,926 + +0.4 3,917,927 - +1.1 3,917,927 - -0.7 3,917,927 - -0.3 3,917,927 - -0.5 3,917,972 + -0.0 3,917,972 + +0.3 3,917,972 + -0.2 3,917,973 - +0.4 3,917,973 - +0.1 3,918,105 + -0.4 3,918,105 + +1.0 3,918,105 + +0.2 3,918,106 - +1.0 3,918,106 - -0.1 3,918,106 - +0.1 3,918,106 - -1.5 3,918,111 + +0.5 3,918,111 + +0.7 3,918,112 - +0.3 3,918,112 - -0.1 3,918,172 + Pf6N2E2_3386 0.29 +0.7 3,918,173 - Pf6N2E2_3386 0.29 -0.2 3,918,173 - Pf6N2E2_3386 0.29 +0.2 3,918,173 - Pf6N2E2_3386 0.29 -0.1 3,918,210 - Pf6N2E2_3386 0.42 +1.8 3,918,210 - Pf6N2E2_3386 0.42 +1.1 3,918,210 - Pf6N2E2_3386 0.42 +0.3 3,918,232 + Pf6N2E2_3386 0.50 +1.0 3,918,233 - Pf6N2E2_3386 0.51 -0.0 3,918,358 + -0.2 3,918,358 + +0.4 3,918,358 + -0.8 3,918,359 - -0.4 3,918,871 - -1.3 3,918,997 + -1.0 3,919,050 + +0.5 3,919,050 + +1.4 3,919,051 - -0.2 3,919,105 - -0.3 3,919,105 - +0.1 3,919,105 - +0.1 3,919,105 - +0.3 3,919,119 + -0.3 3,919,328 + Pf6N2E2_3387 0.23 +0.2 3,919,328 + Pf6N2E2_3387 0.23 +0.8 3,919,328 + Pf6N2E2_3387 0.23 +0.0 3,919,329 - Pf6N2E2_3387 0.23 -0.2 3,919,329 - Pf6N2E2_3387 0.23 +0.1 3,919,329 - Pf6N2E2_3387 0.23 +0.2
Or see this region's nucleotide sequence