Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_251

Experiment: L-Lysine 25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_250 and Pf6N2E2_251 are separated by 184 nucleotidesPf6N2E2_251 and Pf6N2E2_252 overlap by 39 nucleotides Pf6N2E2_250 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 311,560 to 312,495 _250 Pf6N2E2_251 - transcriptional regulator, at 312,680 to 314,167 _251 Pf6N2E2_252 - Disease resistance protein / Tetratricopeptide repeat-containing protein, at 314,129 to 316,918 _252 Position (kb) 312 313 314 315Strain fitness (log2 ratio) -1 0 1at 311.753 kb on - strand, within Pf6N2E2_250at 311.788 kb on + strand, within Pf6N2E2_250at 311.788 kb on + strand, within Pf6N2E2_250at 311.788 kb on + strand, within Pf6N2E2_250at 311.788 kb on + strand, within Pf6N2E2_250at 311.933 kb on - strand, within Pf6N2E2_250at 311.987 kb on + strand, within Pf6N2E2_250at 311.987 kb on + strand, within Pf6N2E2_250at 311.987 kb on + strand, within Pf6N2E2_250at 311.987 kb on + strand, within Pf6N2E2_250at 312.578 kb on + strandat 312.619 kb on - strandat 312.653 kb on + strandat 312.839 kb on + strand, within Pf6N2E2_251at 312.962 kb on + strand, within Pf6N2E2_251at 312.962 kb on + strandat 312.962 kb on + strand, within Pf6N2E2_251at 312.963 kb on - strand, within Pf6N2E2_251at 312.989 kb on + strand, within Pf6N2E2_251at 313.734 kb on + strand, within Pf6N2E2_251at 313.735 kb on - strand, within Pf6N2E2_251at 313.735 kb on - strand, within Pf6N2E2_251at 313.929 kb on + strand, within Pf6N2E2_251at 314.027 kb on + strandat 314.027 kb on + strandat 314.028 kb on - strandat 314.028 kb on - strandat 314.028 kb on - strandat 314.028 kb on - strandat 314.082 kb on - strandat 314.655 kb on + strand, within Pf6N2E2_252at 314.691 kb on + strand, within Pf6N2E2_252at 314.827 kb on - strand, within Pf6N2E2_252at 314.827 kb on - strand, within Pf6N2E2_252at 315.036 kb on + strand, within Pf6N2E2_252at 315.056 kb on + strand, within Pf6N2E2_252at 315.056 kb on + strand, within Pf6N2E2_252

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lysine 25 mM
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311,753 - Pf6N2E2_250 0.21 -1.5
311,788 + Pf6N2E2_250 0.24 -0.3
311,788 + Pf6N2E2_250 0.24 +0.2
311,788 + Pf6N2E2_250 0.24 -0.8
311,788 + Pf6N2E2_250 0.24 -0.1
311,933 - Pf6N2E2_250 0.40 -0.1
311,987 + Pf6N2E2_250 0.46 +0.3
311,987 + Pf6N2E2_250 0.46 +0.3
311,987 + Pf6N2E2_250 0.46 -0.7
311,987 + Pf6N2E2_250 0.46 +1.1
312,578 + -0.0
312,619 - +0.1
312,653 + +0.6
312,839 + Pf6N2E2_251 0.11 +0.7
312,962 + Pf6N2E2_251 0.19 +1.0
312,962 + +1.6
312,962 + Pf6N2E2_251 0.19 +0.4
312,963 - Pf6N2E2_251 0.19 +0.7
312,989 + Pf6N2E2_251 0.21 +1.2
313,734 + Pf6N2E2_251 0.71 -0.5
313,735 - Pf6N2E2_251 0.71 +0.7
313,735 - Pf6N2E2_251 0.71 +0.5
313,929 + Pf6N2E2_251 0.84 +0.5
314,027 + -0.1
314,027 + -0.5
314,028 - -1.0
314,028 - +0.1
314,028 - -0.5
314,028 - +0.9
314,082 - +0.6
314,655 + Pf6N2E2_252 0.19 -0.1
314,691 + Pf6N2E2_252 0.20 -0.3
314,827 - Pf6N2E2_252 0.25 -0.3
314,827 - Pf6N2E2_252 0.25 -0.4
315,036 + Pf6N2E2_252 0.33 -0.1
315,056 + Pf6N2E2_252 0.33 -0.0
315,056 + Pf6N2E2_252 0.33 +0.1

Or see this region's nucleotide sequence