Experiment: L-Lysine 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2194 and Pf6N2E2_2195 are separated by 29 nucleotides Pf6N2E2_2195 and Pf6N2E2_2196 are separated by 122 nucleotides Pf6N2E2_2196 and Pf6N2E2_2197 are separated by 499 nucleotides
Pf6N2E2_2194 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 2,600,323 to 2,602,272
_2194
Pf6N2E2_2195 - cI, at 2,602,302 to 2,602,958
_2195
Pf6N2E2_2196 - FIG00953347: hypothetical protein, at 2,603,081 to 2,603,422
_2196
Pf6N2E2_2197 - Predicted carboxypeptidase, at 2,603,922 to 2,605,067
_2197
Position (kb)
2603
2604 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2602.089 kb on + strand at 2602.090 kb on - strand at 2602.090 kb on - strand at 2602.213 kb on + strand at 2602.213 kb on + strand at 2602.214 kb on - strand at 2602.270 kb on + strand at 2602.270 kb on + strand at 2602.357 kb on + strand at 2602.357 kb on + strand at 2602.358 kb on - strand at 2602.397 kb on - strand, within Pf6N2E2_2195 at 2602.627 kb on + strand, within Pf6N2E2_2195 at 2602.628 kb on - strand, within Pf6N2E2_2195 at 2602.703 kb on - strand, within Pf6N2E2_2195 at 2602.936 kb on - strand at 2602.943 kb on + strand at 2602.944 kb on - strand at 2602.944 kb on - strand at 2602.961 kb on + strand at 2602.962 kb on - strand at 2602.962 kb on - strand at 2603.086 kb on + strand at 2603.086 kb on + strand at 2603.087 kb on - strand at 2603.175 kb on - strand, within Pf6N2E2_2196 at 2603.175 kb on - strand, within Pf6N2E2_2196 at 2603.175 kb on - strand, within Pf6N2E2_2196 at 2603.177 kb on + strand, within Pf6N2E2_2196 at 2603.177 kb on + strand, within Pf6N2E2_2196 at 2603.177 kb on + strand, within Pf6N2E2_2196 at 2603.178 kb on - strand, within Pf6N2E2_2196 at 2603.178 kb on - strand, within Pf6N2E2_2196 at 2603.517 kb on - strand at 2603.550 kb on + strand at 2603.565 kb on + strand at 2603.565 kb on + strand at 2603.565 kb on + strand at 2603.565 kb on + strand at 2603.565 kb on + strand at 2603.565 kb on + strand at 2603.566 kb on - strand at 2603.583 kb on + strand at 2603.583 kb on + strand at 2603.583 kb on + strand at 2603.583 kb on + strand at 2603.657 kb on - strand at 2603.713 kb on + strand at 2603.724 kb on + strand at 2603.764 kb on + strand at 2603.765 kb on - strand at 2603.803 kb on + strand at 2603.804 kb on - strand at 2603.804 kb on - strand at 2603.841 kb on + strand at 2603.842 kb on - strand at 2603.842 kb on - strand at 2603.842 kb on - strand at 2603.850 kb on + strand at 2603.850 kb on + strand at 2603.851 kb on - strand at 2603.851 kb on - strand at 2603.881 kb on + strand at 2603.881 kb on + strand at 2603.881 kb on + strand at 2603.881 kb on + strand at 2603.882 kb on - strand at 2603.882 kb on - strand at 2603.882 kb on - strand at 2603.886 kb on + strand at 2603.886 kb on + strand at 2603.887 kb on - strand at 2603.927 kb on + strand at 2603.943 kb on - strand at 2604.090 kb on + strand, within Pf6N2E2_2197 at 2604.090 kb on + strand, within Pf6N2E2_2197 at 2604.090 kb on + strand, within Pf6N2E2_2197 at 2604.090 kb on + strand, within Pf6N2E2_2197 at 2604.127 kb on - strand, within Pf6N2E2_2197 at 2604.165 kb on + strand, within Pf6N2E2_2197 at 2604.166 kb on - strand, within Pf6N2E2_2197 at 2604.261 kb on + strand, within Pf6N2E2_2197 at 2604.273 kb on + strand, within Pf6N2E2_2197 at 2604.274 kb on - strand, within Pf6N2E2_2197 at 2604.274 kb on - strand, within Pf6N2E2_2197
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 25 mM remove 2,602,089 + -0.2 2,602,090 - +0.5 2,602,090 - -1.7 2,602,213 + -0.4 2,602,213 + -0.3 2,602,214 - -0.3 2,602,270 + -0.3 2,602,270 + -0.1 2,602,357 + +0.5 2,602,357 + +0.5 2,602,358 - +1.3 2,602,397 - Pf6N2E2_2195 0.14 +0.3 2,602,627 + Pf6N2E2_2195 0.49 -0.1 2,602,628 - Pf6N2E2_2195 0.50 +0.8 2,602,703 - Pf6N2E2_2195 0.61 -0.9 2,602,936 - +0.9 2,602,943 + +0.7 2,602,944 - -0.0 2,602,944 - +0.8 2,602,961 + +0.1 2,602,962 - +0.3 2,602,962 - -0.4 2,603,086 + -0.1 2,603,086 + +0.6 2,603,087 - +0.2 2,603,175 - Pf6N2E2_2196 0.27 +0.0 2,603,175 - Pf6N2E2_2196 0.27 -1.0 2,603,175 - Pf6N2E2_2196 0.27 +0.3 2,603,177 + Pf6N2E2_2196 0.28 +1.1 2,603,177 + Pf6N2E2_2196 0.28 +0.1 2,603,177 + Pf6N2E2_2196 0.28 +0.9 2,603,178 - Pf6N2E2_2196 0.28 +0.3 2,603,178 - Pf6N2E2_2196 0.28 +0.8 2,603,517 - +1.0 2,603,550 + -0.9 2,603,565 + -0.1 2,603,565 + +0.5 2,603,565 + -1.5 2,603,565 + -1.4 2,603,565 + +0.1 2,603,565 + -0.4 2,603,566 - -0.4 2,603,583 + -0.2 2,603,583 + +0.7 2,603,583 + +0.0 2,603,583 + -0.6 2,603,657 - +0.2 2,603,713 + -2.3 2,603,724 + -0.5 2,603,764 + -1.0 2,603,765 - -0.5 2,603,803 + -0.1 2,603,804 - +0.2 2,603,804 - +0.4 2,603,841 + +0.4 2,603,842 - -0.1 2,603,842 - +0.0 2,603,842 - +0.8 2,603,850 + +0.3 2,603,850 + -0.3 2,603,851 - -0.4 2,603,851 - +0.1 2,603,881 + +1.1 2,603,881 + -0.2 2,603,881 + -3.1 2,603,881 + +1.1 2,603,882 - +0.5 2,603,882 - +0.4 2,603,882 - -0.3 2,603,886 + +0.1 2,603,886 + +1.2 2,603,887 - -0.1 2,603,927 + -1.6 2,603,943 - +0.0 2,604,090 + Pf6N2E2_2197 0.15 -0.1 2,604,090 + Pf6N2E2_2197 0.15 +0.1 2,604,090 + Pf6N2E2_2197 0.15 -0.0 2,604,090 + Pf6N2E2_2197 0.15 +0.2 2,604,127 - Pf6N2E2_2197 0.18 -0.2 2,604,165 + Pf6N2E2_2197 0.21 -0.1 2,604,166 - Pf6N2E2_2197 0.21 -1.2 2,604,261 + Pf6N2E2_2197 0.30 +0.3 2,604,273 + Pf6N2E2_2197 0.31 +0.7 2,604,274 - Pf6N2E2_2197 0.31 +1.2 2,604,274 - Pf6N2E2_2197 0.31 -0.4
Or see this region's nucleotide sequence