Experiment: L-Lysine 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2100 and Pf6N2E2_2101 are separated by 783 nucleotides Pf6N2E2_2101 and Pf6N2E2_2102 are separated by 62 nucleotides
Pf6N2E2_2100 - DNA topoisomerase III (EC 5.99.1.2), at 2,498,254 to 2,500,200
_2100
Pf6N2E2_2101 - hypothetical protein, at 2,500,984 to 2,501,202
_2101
Pf6N2E2_2102 - Glutaminase (EC 3.5.1.2) (from data) , at 2,501,265 to 2,502,173
_2102
Position (kb)
2500
2501
2502 Strain fitness (log2 ratio)
-2
-1
0
1 at 2500.204 kb on + strand at 2500.204 kb on + strand at 2500.204 kb on + strand at 2500.204 kb on + strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.408 kb on - strand at 2500.408 kb on - strand at 2500.408 kb on - strand at 2500.408 kb on - strand at 2500.408 kb on - strand at 2500.411 kb on + strand at 2500.412 kb on - strand at 2500.412 kb on - strand at 2500.425 kb on - strand at 2500.432 kb on - strand at 2500.494 kb on - strand at 2500.699 kb on + strand at 2500.700 kb on - strand at 2500.713 kb on - strand at 2500.732 kb on + strand at 2500.732 kb on + strand at 2500.732 kb on + strand at 2500.732 kb on + strand at 2500.733 kb on - strand at 2500.733 kb on - strand at 2500.733 kb on - strand at 2500.805 kb on + strand at 2500.805 kb on + strand at 2500.806 kb on - strand at 2500.806 kb on - strand at 2500.806 kb on - strand at 2500.806 kb on - strand at 2500.806 kb on - strand at 2500.809 kb on + strand at 2500.809 kb on + strand at 2500.810 kb on - strand at 2500.810 kb on - strand at 2500.868 kb on + strand at 2500.868 kb on + strand at 2500.868 kb on + strand at 2500.868 kb on + strand at 2500.869 kb on - strand at 2500.869 kb on - strand at 2500.869 kb on - strand at 2500.869 kb on - strand at 2500.869 kb on - strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.916 kb on + strand at 2500.917 kb on - strand at 2500.950 kb on + strand at 2500.983 kb on + strand at 2501.002 kb on + strand at 2501.062 kb on + strand, within Pf6N2E2_2101 at 2501.063 kb on - strand, within Pf6N2E2_2101 at 2501.080 kb on + strand, within Pf6N2E2_2101 at 2501.080 kb on + strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.266 kb on + strand at 2501.267 kb on - strand at 2501.462 kb on - strand, within Pf6N2E2_2102 at 2501.462 kb on - strand, within Pf6N2E2_2102 at 2501.462 kb on - strand, within Pf6N2E2_2102 at 2501.462 kb on - strand, within Pf6N2E2_2102 at 2501.617 kb on + strand, within Pf6N2E2_2102 at 2501.617 kb on + strand, within Pf6N2E2_2102 at 2501.674 kb on + strand, within Pf6N2E2_2102 at 2501.674 kb on + strand, within Pf6N2E2_2102 at 2501.675 kb on - strand, within Pf6N2E2_2102 at 2501.675 kb on - strand, within Pf6N2E2_2102 at 2501.757 kb on + strand, within Pf6N2E2_2102 at 2501.758 kb on - strand, within Pf6N2E2_2102 at 2501.847 kb on - strand, within Pf6N2E2_2102 at 2501.847 kb on - strand, within Pf6N2E2_2102 at 2501.991 kb on - strand, within Pf6N2E2_2102 at 2502.052 kb on + strand, within Pf6N2E2_2102 at 2502.173 kb on + strand at 2502.173 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 25 mM remove 2,500,204 + -0.1 2,500,204 + -0.1 2,500,204 + -0.2 2,500,204 + -0.8 2,500,205 - +0.3 2,500,205 - +0.2 2,500,205 - -0.1 2,500,205 - +0.1 2,500,205 - -0.1 2,500,205 - +0.3 2,500,205 - -0.1 2,500,205 - -0.8 2,500,205 - +0.1 2,500,408 - -2.5 2,500,408 - +0.1 2,500,408 - -0.5 2,500,408 - +0.0 2,500,408 - -0.8 2,500,411 + +0.3 2,500,412 - +0.0 2,500,412 - -0.6 2,500,425 - -0.2 2,500,432 - -0.4 2,500,494 - +0.3 2,500,699 + +0.1 2,500,700 - +0.5 2,500,713 - +0.8 2,500,732 + +0.2 2,500,732 + +0.5 2,500,732 + +1.1 2,500,732 + -0.1 2,500,733 - +0.3 2,500,733 - -0.2 2,500,733 - -1.5 2,500,805 + +0.8 2,500,805 + -0.4 2,500,806 - -0.4 2,500,806 - +0.7 2,500,806 - +0.1 2,500,806 - -0.4 2,500,806 - -1.6 2,500,809 + -0.2 2,500,809 + +0.8 2,500,810 - +0.1 2,500,810 - +1.7 2,500,868 + +0.2 2,500,868 + +0.7 2,500,868 + -0.2 2,500,868 + -0.1 2,500,869 - +1.3 2,500,869 - +1.0 2,500,869 - +0.3 2,500,869 - -0.2 2,500,869 - +0.1 2,500,896 + -0.0 2,500,896 + +0.5 2,500,896 + -0.2 2,500,896 + -0.1 2,500,896 + +1.1 2,500,896 + +0.3 2,500,896 + +0.4 2,500,896 + +0.2 2,500,896 + -0.5 2,500,896 + -0.1 2,500,897 - -0.2 2,500,897 - +0.5 2,500,897 - +0.6 2,500,897 - +0.1 2,500,897 - -0.3 2,500,897 - +0.1 2,500,897 - +0.1 2,500,897 - +0.2 2,500,897 - -0.3 2,500,916 + +0.1 2,500,917 - +0.8 2,500,950 + -0.2 2,500,983 + -0.1 2,501,002 + +0.2 2,501,062 + Pf6N2E2_2101 0.36 +0.6 2,501,063 - Pf6N2E2_2101 0.36 -0.9 2,501,080 + Pf6N2E2_2101 0.44 +0.5 2,501,080 + Pf6N2E2_2101 0.44 +1.1 2,501,081 - Pf6N2E2_2101 0.44 -0.1 2,501,081 - Pf6N2E2_2101 0.44 +0.3 2,501,081 - Pf6N2E2_2101 0.44 +0.6 2,501,081 - Pf6N2E2_2101 0.44 +0.8 2,501,081 - Pf6N2E2_2101 0.44 +1.1 2,501,081 - Pf6N2E2_2101 0.44 +0.6 2,501,266 + -0.0 2,501,267 - -0.3 2,501,462 - Pf6N2E2_2102 0.22 -0.7 2,501,462 - Pf6N2E2_2102 0.22 -0.9 2,501,462 - Pf6N2E2_2102 0.22 -0.8 2,501,462 - Pf6N2E2_2102 0.22 -0.4 2,501,617 + Pf6N2E2_2102 0.39 -1.5 2,501,617 + Pf6N2E2_2102 0.39 -0.1 2,501,674 + Pf6N2E2_2102 0.45 -0.4 2,501,674 + Pf6N2E2_2102 0.45 -0.0 2,501,675 - Pf6N2E2_2102 0.45 -0.5 2,501,675 - Pf6N2E2_2102 0.45 -0.1 2,501,757 + Pf6N2E2_2102 0.54 +0.6 2,501,758 - Pf6N2E2_2102 0.54 +0.5 2,501,847 - Pf6N2E2_2102 0.64 -0.0 2,501,847 - Pf6N2E2_2102 0.64 +0.0 2,501,991 - Pf6N2E2_2102 0.80 +0.5 2,502,052 + Pf6N2E2_2102 0.87 -0.2 2,502,173 + -0.7 2,502,173 + +0.0
Or see this region's nucleotide sequence