Experiment: L-Lysine 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1916 and Pf6N2E2_1917 overlap by 11 nucleotides Pf6N2E2_1917 and Pf6N2E2_1918 are separated by 498 nucleotides Pf6N2E2_1918 and Pf6N2E2_1919 are separated by 205 nucleotides
Pf6N2E2_1916 - Transcriptional regulator, LysR family, at 2,270,692 to 2,270,970
_1916
Pf6N2E2_1917 - hypothetical protein, at 2,270,960 to 2,271,241
_1917
Pf6N2E2_1918 - Major facilitator family transporter, at 2,271,740 to 2,272,900
_1918
Pf6N2E2_1919 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,273,106 to 2,274,578
_1919
Position (kb)
2271
2272
2273 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2270.839 kb on - strand, within Pf6N2E2_1916 at 2270.839 kb on - strand, within Pf6N2E2_1916 at 2270.839 kb on - strand, within Pf6N2E2_1916 at 2270.860 kb on + strand, within Pf6N2E2_1916 at 2270.861 kb on - strand, within Pf6N2E2_1916 at 2270.861 kb on - strand, within Pf6N2E2_1916 at 2271.155 kb on - strand, within Pf6N2E2_1917 at 2271.156 kb on + strand, within Pf6N2E2_1917 at 2271.157 kb on - strand, within Pf6N2E2_1917 at 2271.157 kb on - strand, within Pf6N2E2_1917 at 2271.256 kb on + strand at 2271.257 kb on - strand at 2271.257 kb on - strand at 2271.272 kb on - strand at 2271.398 kb on + strand at 2271.425 kb on - strand at 2271.441 kb on - strand at 2271.571 kb on - strand at 2271.725 kb on - strand at 2271.725 kb on - strand at 2271.725 kb on - strand at 2271.727 kb on - strand at 2271.727 kb on - strand at 2271.737 kb on - strand at 2271.828 kb on - strand at 2271.828 kb on - strand at 2271.828 kb on - strand at 2271.828 kb on - strand at 2271.828 kb on - strand at 2271.828 kb on - strand at 2271.840 kb on + strand at 2271.841 kb on - strand at 2271.841 kb on - strand at 2271.846 kb on - strand at 2271.928 kb on + strand, within Pf6N2E2_1918 at 2271.968 kb on - strand, within Pf6N2E2_1918 at 2272.102 kb on + strand, within Pf6N2E2_1918 at 2272.102 kb on + strand, within Pf6N2E2_1918 at 2272.102 kb on + strand, within Pf6N2E2_1918 at 2272.103 kb on - strand, within Pf6N2E2_1918 at 2272.128 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.221 kb on + strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.231 kb on - strand, within Pf6N2E2_1918 at 2272.398 kb on + strand, within Pf6N2E2_1918 at 2272.436 kb on - strand at 2272.605 kb on + strand, within Pf6N2E2_1918 at 2272.628 kb on - strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.737 kb on + strand, within Pf6N2E2_1918 at 2272.737 kb on + strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.847 kb on - strand at 2272.973 kb on + strand at 2272.973 kb on + strand at 2273.018 kb on + strand at 2273.019 kb on - strand at 2273.127 kb on + strand at 2273.149 kb on + strand at 2273.149 kb on + strand at 2273.149 kb on + strand at 2273.150 kb on - strand at 2273.334 kb on + strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.482 kb on - strand, within Pf6N2E2_1919 at 2273.482 kb on - strand, within Pf6N2E2_1919 at 2273.617 kb on - strand, within Pf6N2E2_1919 at 2273.659 kb on + strand, within Pf6N2E2_1919 at 2273.660 kb on - strand, within Pf6N2E2_1919 at 2273.664 kb on - strand, within Pf6N2E2_1919 at 2273.664 kb on - strand, within Pf6N2E2_1919
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 25 mM remove 2,270,839 - Pf6N2E2_1916 0.53 +0.2 2,270,839 - Pf6N2E2_1916 0.53 +0.1 2,270,839 - Pf6N2E2_1916 0.53 +0.4 2,270,860 + Pf6N2E2_1916 0.60 +1.7 2,270,861 - Pf6N2E2_1916 0.61 -0.0 2,270,861 - Pf6N2E2_1916 0.61 -1.0 2,271,155 - Pf6N2E2_1917 0.69 -0.7 2,271,156 + Pf6N2E2_1917 0.70 +0.5 2,271,157 - Pf6N2E2_1917 0.70 +0.2 2,271,157 - Pf6N2E2_1917 0.70 +0.2 2,271,256 + +0.9 2,271,257 - +0.3 2,271,257 - -0.3 2,271,272 - +0.6 2,271,398 + -0.5 2,271,425 - -0.8 2,271,441 - -1.4 2,271,571 - +0.0 2,271,725 - +0.5 2,271,725 - +0.2 2,271,725 - +0.2 2,271,727 - +0.2 2,271,727 - +1.1 2,271,737 - -1.1 2,271,828 - +0.3 2,271,828 - -0.1 2,271,828 - -0.1 2,271,828 - +0.1 2,271,828 - -0.5 2,271,828 - +0.0 2,271,840 + -0.3 2,271,841 - -0.3 2,271,841 - +1.5 2,271,846 - +0.0 2,271,928 + Pf6N2E2_1918 0.16 -0.8 2,271,968 - Pf6N2E2_1918 0.20 +1.1 2,272,102 + Pf6N2E2_1918 0.31 -0.4 2,272,102 + Pf6N2E2_1918 0.31 -0.2 2,272,102 + Pf6N2E2_1918 0.31 +0.1 2,272,103 - Pf6N2E2_1918 0.31 +0.0 2,272,128 - Pf6N2E2_1918 0.33 +0.3 2,272,216 - Pf6N2E2_1918 0.41 -0.8 2,272,216 - Pf6N2E2_1918 0.41 -0.2 2,272,216 - Pf6N2E2_1918 0.41 +0.3 2,272,221 + Pf6N2E2_1918 0.41 +0.2 2,272,222 - Pf6N2E2_1918 0.42 -0.4 2,272,222 - Pf6N2E2_1918 0.42 +0.0 2,272,222 - Pf6N2E2_1918 0.42 +0.4 2,272,222 - Pf6N2E2_1918 0.42 -0.5 2,272,231 - Pf6N2E2_1918 0.42 -0.1 2,272,398 + Pf6N2E2_1918 0.57 +0.4 2,272,436 - +1.9 2,272,605 + Pf6N2E2_1918 0.75 +0.8 2,272,628 - Pf6N2E2_1918 0.76 -2.7 2,272,665 + Pf6N2E2_1918 0.80 +1.5 2,272,665 + Pf6N2E2_1918 0.80 +0.3 2,272,665 + Pf6N2E2_1918 0.80 -0.3 2,272,665 + Pf6N2E2_1918 0.80 +0.4 2,272,665 + Pf6N2E2_1918 0.80 -0.1 2,272,666 - Pf6N2E2_1918 0.80 +0.6 2,272,666 - Pf6N2E2_1918 0.80 +0.5 2,272,666 - Pf6N2E2_1918 0.80 +0.2 2,272,666 - Pf6N2E2_1918 0.80 -0.3 2,272,737 + Pf6N2E2_1918 0.86 +0.2 2,272,737 + Pf6N2E2_1918 0.86 -0.0 2,272,738 - Pf6N2E2_1918 0.86 +0.9 2,272,738 - Pf6N2E2_1918 0.86 +0.1 2,272,738 - Pf6N2E2_1918 0.86 -0.1 2,272,738 - Pf6N2E2_1918 0.86 +0.3 2,272,738 - Pf6N2E2_1918 0.86 -0.8 2,272,738 - Pf6N2E2_1918 0.86 +0.2 2,272,738 - Pf6N2E2_1918 0.86 +0.0 2,272,847 - +0.0 2,272,973 + -0.5 2,272,973 + +0.3 2,273,018 + -1.0 2,273,019 - -0.6 2,273,127 + +0.9 2,273,149 + +0.7 2,273,149 + +1.3 2,273,149 + +0.8 2,273,150 - +0.3 2,273,334 + Pf6N2E2_1919 0.15 -0.0 2,273,431 - Pf6N2E2_1919 0.22 +0.4 2,273,431 - Pf6N2E2_1919 0.22 -0.4 2,273,431 - Pf6N2E2_1919 0.22 -0.5 2,273,482 - Pf6N2E2_1919 0.26 +0.7 2,273,482 - Pf6N2E2_1919 0.26 +0.7 2,273,617 - Pf6N2E2_1919 0.35 +0.2 2,273,659 + Pf6N2E2_1919 0.38 +0.2 2,273,660 - Pf6N2E2_1919 0.38 -0.7 2,273,664 - Pf6N2E2_1919 0.38 +1.5 2,273,664 - Pf6N2E2_1919 0.38 +0.9
Or see this region's nucleotide sequence