Experiment: L-Lysine 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1437 and Pf6N2E2_1438 are separated by 527 nucleotides Pf6N2E2_1438 and Pf6N2E2_1439 are separated by 237 nucleotides
Pf6N2E2_1437 - Acyl-CoA dehydrogenase family protein, at 1,686,776 to 1,688,020
_1437
Pf6N2E2_1438 - Methyltransferase (EC 2.1.1.-), at 1,688,548 to 1,689,132
_1438
Pf6N2E2_1439 - hypothetical protein, at 1,689,370 to 1,689,948
_1439
Position (kb)
1688
1689
1690 Strain fitness (log2 ratio)
-2
-1
0
1 at 1687.662 kb on - strand, within Pf6N2E2_1437 at 1687.712 kb on + strand, within Pf6N2E2_1437 at 1687.931 kb on + strand at 1687.931 kb on + strand at 1687.931 kb on + strand at 1687.932 kb on - strand at 1687.982 kb on + strand at 1687.983 kb on - strand at 1688.022 kb on + strand at 1688.022 kb on + strand at 1688.066 kb on + strand at 1688.067 kb on - strand at 1688.215 kb on + strand at 1688.216 kb on - strand at 1688.216 kb on - strand at 1688.282 kb on - strand at 1688.282 kb on - strand at 1688.298 kb on - strand at 1688.323 kb on - strand at 1688.323 kb on - strand at 1688.331 kb on + strand at 1688.332 kb on - strand at 1688.391 kb on - strand at 1688.391 kb on - strand at 1688.391 kb on - strand at 1688.396 kb on - strand at 1688.416 kb on + strand at 1688.416 kb on + strand at 1688.417 kb on - strand at 1688.417 kb on - strand at 1688.417 kb on - strand at 1688.450 kb on + strand at 1688.507 kb on + strand at 1688.508 kb on - strand at 1688.744 kb on + strand, within Pf6N2E2_1438 at 1688.745 kb on - strand, within Pf6N2E2_1438 at 1688.821 kb on - strand, within Pf6N2E2_1438 at 1688.821 kb on - strand, within Pf6N2E2_1438 at 1688.930 kb on - strand, within Pf6N2E2_1438 at 1688.988 kb on + strand, within Pf6N2E2_1438 at 1688.989 kb on - strand, within Pf6N2E2_1438 at 1689.095 kb on - strand at 1689.109 kb on + strand at 1689.110 kb on - strand at 1689.234 kb on - strand at 1689.234 kb on - strand at 1689.324 kb on - strand at 1689.379 kb on + strand at 1689.380 kb on - strand at 1689.445 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.672 kb on - strand, within Pf6N2E2_1439 at 1689.672 kb on - strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.706 kb on + strand, within Pf6N2E2_1439 at 1689.707 kb on - strand, within Pf6N2E2_1439 at 1689.781 kb on + strand, within Pf6N2E2_1439 at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.098 kb on - strand at 1690.098 kb on - strand at 1690.098 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 25 mM remove 1,687,662 - Pf6N2E2_1437 0.71 -0.9 1,687,712 + Pf6N2E2_1437 0.75 +0.3 1,687,931 + +0.7 1,687,931 + +0.8 1,687,931 + +0.4 1,687,932 - +0.1 1,687,982 + +0.9 1,687,983 - -0.4 1,688,022 + +0.6 1,688,022 + +0.9 1,688,066 + +0.1 1,688,067 - +0.7 1,688,215 + -1.6 1,688,216 - +0.6 1,688,216 - -1.1 1,688,282 - -0.5 1,688,282 - +0.5 1,688,298 - +0.7 1,688,323 - -0.7 1,688,323 - +0.1 1,688,331 + -0.7 1,688,332 - -0.3 1,688,391 - -0.8 1,688,391 - +0.3 1,688,391 - -0.9 1,688,396 - +0.7 1,688,416 + +0.4 1,688,416 + -0.1 1,688,417 - +0.4 1,688,417 - -2.1 1,688,417 - -0.4 1,688,450 + +0.2 1,688,507 + +1.3 1,688,508 - -0.1 1,688,744 + Pf6N2E2_1438 0.34 +0.8 1,688,745 - Pf6N2E2_1438 0.34 +1.5 1,688,821 - Pf6N2E2_1438 0.47 +1.4 1,688,821 - Pf6N2E2_1438 0.47 +0.1 1,688,930 - Pf6N2E2_1438 0.65 +0.7 1,688,988 + Pf6N2E2_1438 0.75 +1.0 1,688,989 - Pf6N2E2_1438 0.75 +0.5 1,689,095 - +0.2 1,689,109 + +0.3 1,689,110 - +0.6 1,689,234 - +1.5 1,689,234 - +0.6 1,689,324 - -0.6 1,689,379 + -1.6 1,689,380 - +0.2 1,689,445 + Pf6N2E2_1439 0.13 -0.0 1,689,481 + Pf6N2E2_1439 0.19 -0.1 1,689,481 + Pf6N2E2_1439 0.19 +1.1 1,689,481 + Pf6N2E2_1439 0.19 +0.4 1,689,481 + Pf6N2E2_1439 0.19 -0.0 1,689,482 - Pf6N2E2_1439 0.19 -1.5 1,689,482 - Pf6N2E2_1439 0.19 +0.3 1,689,482 - Pf6N2E2_1439 0.19 +0.8 1,689,672 - Pf6N2E2_1439 0.52 -1.0 1,689,672 - Pf6N2E2_1439 0.52 -0.5 1,689,685 + Pf6N2E2_1439 0.54 -0.1 1,689,685 + Pf6N2E2_1439 0.54 -0.9 1,689,685 + Pf6N2E2_1439 0.54 +0.2 1,689,685 + Pf6N2E2_1439 0.54 +0.0 1,689,685 + Pf6N2E2_1439 0.54 +0.8 1,689,686 - Pf6N2E2_1439 0.55 +0.3 1,689,686 - Pf6N2E2_1439 0.55 +0.4 1,689,686 - Pf6N2E2_1439 0.55 +0.5 1,689,686 - Pf6N2E2_1439 0.55 -0.4 1,689,706 + Pf6N2E2_1439 0.58 +0.0 1,689,707 - Pf6N2E2_1439 0.58 -0.3 1,689,781 + Pf6N2E2_1439 0.71 +0.8 1,690,094 - +0.1 1,690,094 - +0.8 1,690,094 - -0.2 1,690,094 - +0.6 1,690,094 - +0.8 1,690,094 - -1.2 1,690,098 - +0.1 1,690,098 - +0.4 1,690,098 - +0.7
Or see this region's nucleotide sequence