Experiment: L-Lysine 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1191 and Pf6N2E2_1192 are separated by 630 nucleotides Pf6N2E2_1192 and Pf6N2E2_1193 are separated by 135 nucleotides Pf6N2E2_1193 and Pf6N2E2_1194 are separated by 57 nucleotides
Pf6N2E2_1191 - Thiaminase II (EC 3.5.99.2), at 1,394,451 to 1,395,161
_1191
Pf6N2E2_1192 - Transcriptional regulator, at 1,395,792 to 1,396,019
_1192
Pf6N2E2_1193 - Outer membrane porin, OprD family, at 1,396,155 to 1,396,385
_1193
Pf6N2E2_1194 - hypothetical protein, at 1,396,443 to 1,397,384
_1194
Position (kb)
1395
1396
1397 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1394.799 kb on - strand, within Pf6N2E2_1191 at 1394.799 kb on - strand, within Pf6N2E2_1191 at 1394.800 kb on + strand, within Pf6N2E2_1191 at 1394.800 kb on + strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.801 kb on - strand, within Pf6N2E2_1191 at 1394.847 kb on + strand, within Pf6N2E2_1191 at 1394.848 kb on - strand, within Pf6N2E2_1191 at 1394.848 kb on - strand, within Pf6N2E2_1191 at 1394.982 kb on - strand, within Pf6N2E2_1191 at 1394.982 kb on - strand, within Pf6N2E2_1191 at 1394.989 kb on + strand, within Pf6N2E2_1191 at 1394.990 kb on - strand, within Pf6N2E2_1191 at 1394.990 kb on - strand, within Pf6N2E2_1191 at 1394.998 kb on + strand, within Pf6N2E2_1191 at 1395.056 kb on + strand, within Pf6N2E2_1191 at 1395.057 kb on - strand, within Pf6N2E2_1191 at 1395.057 kb on - strand at 1395.058 kb on + strand, within Pf6N2E2_1191 at 1395.059 kb on - strand, within Pf6N2E2_1191 at 1395.059 kb on - strand, within Pf6N2E2_1191 at 1395.133 kb on + strand at 1395.161 kb on + strand at 1395.161 kb on + strand at 1395.161 kb on + strand at 1395.162 kb on - strand at 1395.162 kb on - strand at 1395.162 kb on - strand at 1395.175 kb on + strand at 1395.176 kb on - strand at 1395.185 kb on + strand at 1395.186 kb on - strand at 1395.186 kb on - strand at 1395.186 kb on - strand at 1395.837 kb on + strand, within Pf6N2E2_1192 at 1395.837 kb on + strand, within Pf6N2E2_1192 at 1395.838 kb on - strand, within Pf6N2E2_1192 at 1395.838 kb on - strand, within Pf6N2E2_1192 at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.906 kb on + strand at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.993 kb on + strand, within Pf6N2E2_1192 at 1395.993 kb on + strand, within Pf6N2E2_1192 at 1396.000 kb on + strand at 1396.000 kb on + strand at 1396.104 kb on + strand at 1396.104 kb on + strand at 1396.105 kb on - strand at 1396.105 kb on - strand at 1396.105 kb on - strand at 1396.111 kb on - strand at 1396.158 kb on + strand at 1396.158 kb on + strand at 1396.220 kb on + strand, within Pf6N2E2_1193 at 1396.220 kb on + strand, within Pf6N2E2_1193 at 1396.233 kb on + strand, within Pf6N2E2_1193 at 1396.233 kb on + strand, within Pf6N2E2_1193 at 1396.233 kb on + strand, within Pf6N2E2_1193 at 1396.234 kb on - strand, within Pf6N2E2_1193 at 1396.303 kb on + strand, within Pf6N2E2_1193 at 1396.328 kb on + strand, within Pf6N2E2_1193 at 1396.329 kb on - strand, within Pf6N2E2_1193 at 1396.329 kb on - strand, within Pf6N2E2_1193 at 1396.329 kb on - strand, within Pf6N2E2_1193 at 1396.352 kb on - strand, within Pf6N2E2_1193 at 1396.395 kb on - strand at 1396.395 kb on - strand at 1396.437 kb on - strand at 1396.509 kb on + strand at 1396.529 kb on + strand at 1396.614 kb on - strand, within Pf6N2E2_1194 at 1396.753 kb on - strand, within Pf6N2E2_1194 at 1396.798 kb on + strand, within Pf6N2E2_1194 at 1396.798 kb on + strand, within Pf6N2E2_1194 at 1396.799 kb on - strand, within Pf6N2E2_1194 at 1396.807 kb on - strand, within Pf6N2E2_1194 at 1396.894 kb on + strand, within Pf6N2E2_1194 at 1396.895 kb on - strand, within Pf6N2E2_1194 at 1396.895 kb on - strand, within Pf6N2E2_1194 at 1396.928 kb on - strand, within Pf6N2E2_1194 at 1397.006 kb on - strand, within Pf6N2E2_1194 at 1397.006 kb on - strand, within Pf6N2E2_1194 at 1397.006 kb on - strand, within Pf6N2E2_1194
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 25 mM remove 1,394,799 - Pf6N2E2_1191 0.49 -1.1 1,394,799 - Pf6N2E2_1191 0.49 -0.5 1,394,800 + Pf6N2E2_1191 0.49 +0.2 1,394,800 + Pf6N2E2_1191 0.49 +0.9 1,394,801 - Pf6N2E2_1191 0.49 -0.5 1,394,801 - Pf6N2E2_1191 0.49 -0.3 1,394,801 - Pf6N2E2_1191 0.49 +0.4 1,394,801 - Pf6N2E2_1191 0.49 -0.0 1,394,801 - Pf6N2E2_1191 0.49 +0.4 1,394,801 - Pf6N2E2_1191 0.49 +1.1 1,394,801 - Pf6N2E2_1191 0.49 +0.1 1,394,801 - Pf6N2E2_1191 0.49 -0.5 1,394,801 - Pf6N2E2_1191 0.49 +0.5 1,394,847 + Pf6N2E2_1191 0.56 +0.5 1,394,848 - Pf6N2E2_1191 0.56 +0.4 1,394,848 - Pf6N2E2_1191 0.56 +1.0 1,394,982 - Pf6N2E2_1191 0.75 +0.1 1,394,982 - Pf6N2E2_1191 0.75 -1.8 1,394,989 + Pf6N2E2_1191 0.76 +0.1 1,394,990 - Pf6N2E2_1191 0.76 +0.0 1,394,990 - Pf6N2E2_1191 0.76 -1.7 1,394,998 + Pf6N2E2_1191 0.77 -0.0 1,395,056 + Pf6N2E2_1191 0.85 +1.0 1,395,057 - Pf6N2E2_1191 0.85 -0.4 1,395,057 - +1.3 1,395,058 + Pf6N2E2_1191 0.85 +0.6 1,395,059 - Pf6N2E2_1191 0.86 -0.1 1,395,059 - Pf6N2E2_1191 0.86 +1.1 1,395,133 + +0.8 1,395,161 + -0.4 1,395,161 + -1.4 1,395,161 + -0.2 1,395,162 - +0.1 1,395,162 - +1.4 1,395,162 - +1.1 1,395,175 + +0.4 1,395,176 - -1.1 1,395,185 + -0.3 1,395,186 - -0.5 1,395,186 - +0.6 1,395,186 - +0.3 1,395,837 + Pf6N2E2_1192 0.20 +1.3 1,395,837 + Pf6N2E2_1192 0.20 +0.0 1,395,838 - Pf6N2E2_1192 0.20 +1.1 1,395,838 - Pf6N2E2_1192 0.20 +1.5 1,395,906 + Pf6N2E2_1192 0.50 +2.1 1,395,906 + Pf6N2E2_1192 0.50 -0.2 1,395,906 + +1.3 1,395,906 + Pf6N2E2_1192 0.50 +0.8 1,395,906 + Pf6N2E2_1192 0.50 +0.1 1,395,907 - Pf6N2E2_1192 0.50 +0.5 1,395,907 - Pf6N2E2_1192 0.50 +0.6 1,395,907 - Pf6N2E2_1192 0.50 +0.9 1,395,907 - Pf6N2E2_1192 0.50 -0.5 1,395,993 + Pf6N2E2_1192 0.88 -1.7 1,395,993 + Pf6N2E2_1192 0.88 +0.4 1,396,000 + +0.3 1,396,000 + -0.1 1,396,104 + +0.3 1,396,104 + +0.6 1,396,105 - +0.5 1,396,105 - +0.3 1,396,105 - -0.3 1,396,111 - +0.4 1,396,158 + +0.8 1,396,158 + -2.2 1,396,220 + Pf6N2E2_1193 0.28 -0.3 1,396,220 + Pf6N2E2_1193 0.28 +0.5 1,396,233 + Pf6N2E2_1193 0.34 -0.8 1,396,233 + Pf6N2E2_1193 0.34 -0.1 1,396,233 + Pf6N2E2_1193 0.34 +1.2 1,396,234 - Pf6N2E2_1193 0.34 -0.1 1,396,303 + Pf6N2E2_1193 0.64 +0.4 1,396,328 + Pf6N2E2_1193 0.75 -0.8 1,396,329 - Pf6N2E2_1193 0.75 -0.9 1,396,329 - Pf6N2E2_1193 0.75 +0.6 1,396,329 - Pf6N2E2_1193 0.75 -1.1 1,396,352 - Pf6N2E2_1193 0.85 +1.0 1,396,395 - +1.0 1,396,395 - +1.4 1,396,437 - +0.0 1,396,509 + +1.7 1,396,529 + -0.2 1,396,614 - Pf6N2E2_1194 0.18 +0.5 1,396,753 - Pf6N2E2_1194 0.33 -0.4 1,396,798 + Pf6N2E2_1194 0.38 -0.1 1,396,798 + Pf6N2E2_1194 0.38 +0.9 1,396,799 - Pf6N2E2_1194 0.38 +0.9 1,396,807 - Pf6N2E2_1194 0.39 -0.0 1,396,894 + Pf6N2E2_1194 0.48 +0.3 1,396,895 - Pf6N2E2_1194 0.48 -0.2 1,396,895 - Pf6N2E2_1194 0.48 +0.2 1,396,928 - Pf6N2E2_1194 0.51 -0.7 1,397,006 - Pf6N2E2_1194 0.60 +0.8 1,397,006 - Pf6N2E2_1194 0.60 +0.2 1,397,006 - Pf6N2E2_1194 0.60 +1.1
Or see this region's nucleotide sequence