Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3485 and folE are separated by 225 nucleotides folE and pyrE are separated by 81 nucleotides pyrE and Shew_3488 are separated by 83 nucleotides Shew_3488 and Shew_3489 are separated by 181 nucleotides
Shew_3485: Shew_3485 - peptidase S9 prolyl oligopeptidase (RefSeq), at 4,170,055 to 4,172,019
_3485
Shew_3486: folE - GTP cyclohydrolase I (RefSeq), at 4,172,245 to 4,172,889
folE
Shew_3487: pyrE - orotate phosphoribosyltransferase (RefSeq), at 4,172,971 to 4,173,612
pyrE
Shew_3488: Shew_3488 - ribonuclease PH (RefSeq), at 4,173,696 to 4,174,409
_3488
Shew_3489: Shew_3489 - hypothetical protein (RefSeq), at 4,174,591 to 4,175,454
_3489
Position (kb)
4172
4173
4174 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4172.116 kb on + strand at 4173.284 kb on - strand, within pyrE at 4173.488 kb on + strand, within pyrE at 4173.488 kb on + strand, within pyrE at 4173.496 kb on - strand, within pyrE at 4173.526 kb on - strand, within pyrE at 4173.556 kb on + strand at 4173.612 kb on - strand at 4173.750 kb on - strand at 4173.897 kb on + strand, within Shew_3488 at 4173.963 kb on - strand, within Shew_3488 at 4173.996 kb on + strand, within Shew_3488 at 4174.001 kb on + strand, within Shew_3488 at 4174.009 kb on - strand, within Shew_3488 at 4174.066 kb on - strand, within Shew_3488 at 4174.306 kb on - strand, within Shew_3488 at 4174.353 kb on - strand at 4174.356 kb on - strand at 4174.358 kb on + strand at 4174.390 kb on - strand at 4174.472 kb on + strand at 4174.530 kb on - strand at 4174.599 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 4,172,116 + +0.6 4,173,284 - pyrE Shew_3487 0.49 +2.3 4,173,488 + pyrE Shew_3487 0.81 +2.6 4,173,488 + pyrE Shew_3487 0.81 +2.3 4,173,496 - pyrE Shew_3487 0.82 +2.3 4,173,526 - pyrE Shew_3487 0.86 +2.4 4,173,556 + +2.3 4,173,612 - +2.3 4,173,750 - -1.0 4,173,897 + Shew_3488 0.28 +1.2 4,173,963 - Shew_3488 0.37 -2.4 4,173,996 + Shew_3488 0.42 +1.3 4,174,001 + Shew_3488 0.43 +1.3 4,174,009 - Shew_3488 0.44 -0.8 4,174,066 - Shew_3488 0.52 -0.8 4,174,306 - Shew_3488 0.85 -0.8 4,174,353 - -0.6 4,174,356 - -1.0 4,174,358 + +1.3 4,174,390 - +0.2 4,174,472 + +0.1 4,174,530 - -0.2 4,174,599 + -0.1
Or see this region's nucleotide sequence