Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3359 and Shew_3360 are separated by 140 nucleotides Shew_3360 and Shew_3361 are separated by 49 nucleotides Shew_3361 and Shew_3362 are separated by 112 nucleotides Shew_3362 and Shew_3363 are separated by 116 nucleotides
Shew_3359: Shew_3359 - hypothetical protein (RefSeq), at 4,009,148 to 4,009,579
_3359
Shew_3360: Shew_3360 - hypothetical protein (RefSeq), at 4,009,720 to 4,009,902
_3360
Shew_3361: Shew_3361 - hypothetical protein (RefSeq), at 4,009,952 to 4,010,335
_3361
Shew_3362: Shew_3362 - hypothetical protein (RefSeq), at 4,010,448 to 4,010,858
_3362
Shew_3363: Shew_3363 - 3-dehydroquinate dehydratase, type II (RefSeq), at 4,010,975 to 4,011,427
_3363
Position (kb)
4009
4010
4011 Strain fitness (log2 ratio)
-1
0
1 at 4008.956 kb on + strand at 4009.048 kb on + strand at 4009.422 kb on + strand, within Shew_3359 at 4009.775 kb on + strand, within Shew_3360 at 4009.775 kb on + strand, within Shew_3360 at 4009.783 kb on - strand, within Shew_3360 at 4009.783 kb on - strand, within Shew_3360 at 4009.864 kb on - strand, within Shew_3360 at 4009.866 kb on + strand, within Shew_3360 at 4009.984 kb on - strand at 4010.012 kb on - strand, within Shew_3361 at 4010.055 kb on - strand, within Shew_3361 at 4010.403 kb on + strand at 4011.162 kb on + strand, within Shew_3363 at 4011.252 kb on - strand, within Shew_3363
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 4,008,956 + -1.0 4,009,048 + +0.3 4,009,422 + Shew_3359 0.63 -0.2 4,009,775 + Shew_3360 0.30 -0.6 4,009,775 + Shew_3360 0.30 -0.2 4,009,783 - Shew_3360 0.34 +0.5 4,009,783 - Shew_3360 0.34 +0.1 4,009,864 - Shew_3360 0.79 +0.1 4,009,866 + Shew_3360 0.80 -0.8 4,009,984 - +0.1 4,010,012 - Shew_3361 0.16 -0.3 4,010,055 - Shew_3361 0.27 -1.7 4,010,403 + +0.6 4,011,162 + Shew_3363 0.41 +0.4 4,011,252 - Shew_3363 0.61 +1.1
Or see this region's nucleotide sequence