Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2570 and Shew_2571 are separated by 89 nucleotides Shew_2571 and Shew_2572 are separated by 48 nucleotides Shew_2572 and Shew_2573 are separated by 57 nucleotides
Shew_2570: Shew_2570 - acyl-CoA dehydrogenase domain-containing protein (RefSeq), at 3,028,464 to 3,029,633
_2570
Shew_2571: Shew_2571 - propionyl-CoA carboxylase (RefSeq), at 3,029,723 to 3,031,330
_2571
Shew_2572: Shew_2572 - enoyl-CoA hydratase/isomerase (RefSeq), at 3,031,379 to 3,032,179
_2572
Shew_2573: Shew_2573 - carbamoyl-phosphate synthase L chain, ATP-binding (RefSeq), at 3,032,237 to 3,034,270
_2573
Position (kb)
3029
3030
3031
3032 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3028.737 kb on - strand, within Shew_2570 at 3028.761 kb on - strand, within Shew_2570 at 3028.765 kb on + strand, within Shew_2570 at 3028.787 kb on + strand, within Shew_2570 at 3028.861 kb on - strand, within Shew_2570 at 3029.217 kb on + strand, within Shew_2570 at 3029.605 kb on + strand at 3029.772 kb on - strand at 3030.097 kb on + strand, within Shew_2571 at 3030.170 kb on - strand, within Shew_2571 at 3030.279 kb on - strand, within Shew_2571 at 3030.297 kb on + strand, within Shew_2571 at 3030.413 kb on + strand, within Shew_2571 at 3030.616 kb on - strand, within Shew_2571 at 3031.040 kb on - strand, within Shew_2571 at 3031.249 kb on - strand at 3031.425 kb on - strand at 3031.542 kb on + strand, within Shew_2572 at 3031.857 kb on + strand, within Shew_2572 at 3032.292 kb on + strand at 3032.310 kb on - strand at 3032.310 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 3,028,737 - Shew_2570 0.23 +0.6 3,028,761 - Shew_2570 0.25 +0.9 3,028,765 + Shew_2570 0.26 +0.7 3,028,787 + Shew_2570 0.28 +0.9 3,028,861 - Shew_2570 0.34 +0.9 3,029,217 + Shew_2570 0.64 +0.8 3,029,605 + +0.8 3,029,772 - +2.0 3,030,097 + Shew_2571 0.23 +1.3 3,030,170 - Shew_2571 0.28 +1.1 3,030,279 - Shew_2571 0.35 -1.4 3,030,297 + Shew_2571 0.36 +1.1 3,030,413 + Shew_2571 0.43 +1.0 3,030,616 - Shew_2571 0.56 +1.5 3,031,040 - Shew_2571 0.82 +0.8 3,031,249 - +1.5 3,031,425 - +1.3 3,031,542 + Shew_2572 0.20 +3.0 3,031,857 + Shew_2572 0.60 +3.4 3,032,292 + +0.9 3,032,310 - +1.5 3,032,310 - +1.3
Or see this region's nucleotide sequence