Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2367 and Shew_2368 are separated by 138 nucleotides Shew_2368 and Shew_2369 are separated by 0 nucleotides Shew_2369 and Shew_2370 are separated by 120 nucleotides Shew_2370 and Shew_2371 are separated by 111 nucleotides
Shew_2367: Shew_2367 - hypothetical protein (RefSeq), at 2,768,570 to 2,769,160
_2367
Shew_2368: Shew_2368 - exonuclease, RNase T and DNA polymerase III (RefSeq), at 2,769,299 to 2,769,979
_2368
Shew_2369: Shew_2369 - cyclic nucleotide-binding protein (RefSeq), at 2,769,980 to 2,771,827
_2369
Shew_2370: Shew_2370 - methyltransferase type 11 (RefSeq), at 2,771,948 to 2,772,607
_2370
Shew_2371: Shew_2371 - actP-like component of L-lactate and L-malate uptake system (from data) , at 2,772,719 to 2,774,437
_2371
Position (kb)
2769
2770
2771
2772 Strain fitness (log2 ratio)
-2
-1
0
1 at 2768.994 kb on + strand, within Shew_2367 at 2769.181 kb on + strand at 2769.189 kb on - strand at 2770.041 kb on - strand at 2770.534 kb on - strand, within Shew_2369 at 2771.485 kb on + strand, within Shew_2369 at 2771.536 kb on + strand, within Shew_2369 at 2771.541 kb on + strand, within Shew_2369 at 2771.596 kb on + strand, within Shew_2369 at 2771.658 kb on + strand at 2771.751 kb on - strand at 2772.566 kb on + strand at 2772.703 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 2,768,994 + Shew_2367 0.72 +1.0 2,769,181 + +1.7 2,769,189 - +0.2 2,770,041 - +1.2 2,770,534 - Shew_2369 0.30 -0.8 2,771,485 + Shew_2369 0.81 -1.5 2,771,536 + Shew_2369 0.84 -0.9 2,771,541 + Shew_2369 0.84 -0.8 2,771,596 + Shew_2369 0.87 +0.2 2,771,658 + -2.1 2,771,751 - -0.1 2,772,566 + +0.5 2,772,703 - +0.2
Or see this region's nucleotide sequence