Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2325 and Shew_2326 overlap by 14 nucleotides Shew_2326 and Shew_2327 are separated by 32 nucleotides Shew_2327 and Shew_2328 are separated by 196 nucleotides Shew_2328 and Shew_2329 overlap by 4 nucleotides Shew_2329 and Shew_2330 are separated by 119 nucleotides
Shew_2325: Shew_2325 - hypothetical protein (RefSeq), at 2,719,779 to 2,720,447
_2325
Shew_2326: Shew_2326 - RepA domain-containing protein (RefSeq), at 2,720,434 to 2,720,778
_2326
Shew_2327: Shew_2327 - hypothetical protein (RefSeq), at 2,720,811 to 2,721,014
_2327
Shew_2328: Shew_2328 - signal transduction histidine kinase, LytS (RefSeq), at 2,721,211 to 2,722,908
_2328
Shew_2329: Shew_2329 - putative two-component response-regulatory protein YehT (RefSeq), at 2,722,905 to 2,723,615
_2329
Shew_2330: Shew_2330 - carbon starvation protein CstA (RefSeq), at 2,723,735 to 2,725,171
_2330
Position (kb)
2721
2722
2723 Strain fitness (log2 ratio)
-1
0
1
2 at 2720.283 kb on - strand, within Shew_2325 at 2720.283 kb on - strand, within Shew_2325 at 2720.867 kb on + strand, within Shew_2327 at 2721.672 kb on + strand, within Shew_2328 at 2721.690 kb on + strand, within Shew_2328 at 2721.698 kb on - strand, within Shew_2328 at 2722.056 kb on - strand, within Shew_2328 at 2722.087 kb on + strand, within Shew_2328 at 2722.600 kb on - strand, within Shew_2328 at 2722.637 kb on - strand, within Shew_2328 at 2722.859 kb on + strand at 2723.139 kb on - strand, within Shew_2329 at 2723.209 kb on - strand, within Shew_2329 at 2723.391 kb on - strand, within Shew_2329 at 2723.890 kb on + strand, within Shew_2330
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 2,720,283 - Shew_2325 0.75 +0.4 2,720,283 - Shew_2325 0.75 +0.0 2,720,867 + Shew_2327 0.27 -0.4 2,721,672 + Shew_2328 0.27 -1.0 2,721,690 + Shew_2328 0.28 +0.7 2,721,698 - Shew_2328 0.29 +2.0 2,722,056 - Shew_2328 0.50 +0.7 2,722,087 + Shew_2328 0.52 +0.4 2,722,600 - Shew_2328 0.82 +0.1 2,722,637 - Shew_2328 0.84 -0.0 2,722,859 + -0.1 2,723,139 - Shew_2329 0.33 +0.2 2,723,209 - Shew_2329 0.43 -0.5 2,723,391 - Shew_2329 0.68 -0.0 2,723,890 + Shew_2330 0.11 -0.0
Or see this region's nucleotide sequence