Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1917 and Shew_1918 are separated by 50 nucleotides Shew_1918 and Shew_1919 are separated by 11 nucleotides Shew_1919 and Shew_1920 are separated by 81 nucleotides Shew_1920 and Shew_1921 are separated by 291 nucleotides Shew_1921 and Shew_1922 are separated by 44 nucleotides
Shew_1917: Shew_1917 - flavodoxin FldA (RefSeq), at 2,215,844 to 2,216,371
_1917
Shew_1918: Shew_1918 - LexA regulated protein (RefSeq), at 2,216,422 to 2,216,694
_1918
Shew_1919: Shew_1919 - hypothetical protein (RefSeq), at 2,216,706 to 2,216,927
_1919
Shew_1920: Shew_1920 - alpha/beta hydrolase fold (RefSeq), at 2,217,009 to 2,217,776
_1920
Shew_1921: Shew_1921 - replication initiation regulator SeqA (RefSeq), at 2,218,068 to 2,218,598
_1921
Shew_1922: Shew_1922 - phosphoglucomutase (RefSeq), at 2,218,643 to 2,220,322
_1922
Position (kb)
2217
2218 Strain fitness (log2 ratio)
-1
0
1 at 2216.658 kb on + strand, within Shew_1918 at 2217.287 kb on - strand, within Shew_1920 at 2217.389 kb on + strand, within Shew_1920 at 2217.489 kb on + strand, within Shew_1920 at 2217.492 kb on - strand, within Shew_1920 at 2217.973 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 2,216,658 + Shew_1918 0.86 -0.2 2,217,287 - Shew_1920 0.36 +1.3 2,217,389 + Shew_1920 0.49 +0.7 2,217,489 + Shew_1920 0.62 +1.0 2,217,492 - Shew_1920 0.63 +0.7 2,217,973 + +0.4
Or see this region's nucleotide sequence