Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1863 and Shew_1864 are separated by 286 nucleotides Shew_1864 and Shew_1865 are separated by 66 nucleotides Shew_1865 and Shew_1866 are separated by 11 nucleotides Shew_1866 and Shew_1867 are separated by 31 nucleotides Shew_1867 and Shew_1868 are separated by 215 nucleotides
Shew_1863: Shew_1863 - arsenate reductase and related (RefSeq), at 2,142,010 to 2,142,354
_1863
Shew_1864: Shew_1864 - glucose-specific PTS system component (RefSeq), at 2,142,641 to 2,143,150
_1864
Shew_1865: Shew_1865 - phosphoenolpyruvate-protein phosphotransferase (RefSeq), at 2,143,217 to 2,144,923
_1865
Shew_1866: Shew_1866 - phosphotransferase system, phosphocarrier protein HPr (RefSeq), at 2,144,935 to 2,145,192
_1866
Shew_1867: Shew_1867 - hypothetical protein (RefSeq), at 2,145,224 to 2,145,616
_1867
Shew_1868: Shew_1868 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 2,145,832 to 2,147,469
_1868
Position (kb)
2143
2144
2145 Strain fitness (log2 ratio)
-1
0
1
2 at 2142.647 kb on - strand at 2142.896 kb on + strand, within Shew_1864 at 2143.003 kb on - strand, within Shew_1864 at 2143.069 kb on + strand, within Shew_1864 at 2143.820 kb on - strand, within Shew_1865 at 2143.822 kb on + strand, within Shew_1865 at 2143.895 kb on + strand, within Shew_1865 at 2144.053 kb on - strand, within Shew_1865 at 2144.293 kb on + strand, within Shew_1865 at 2144.483 kb on - strand, within Shew_1865 at 2145.145 kb on + strand, within Shew_1866 at 2145.418 kb on + strand, within Shew_1867 at 2145.546 kb on + strand, within Shew_1867 at 2145.685 kb on + strand at 2145.874 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 2,142,647 - +0.5 2,142,896 + Shew_1864 0.50 -0.7 2,143,003 - Shew_1864 0.71 -1.0 2,143,069 + Shew_1864 0.84 +0.3 2,143,820 - Shew_1865 0.35 +2.3 2,143,822 + Shew_1865 0.35 +1.3 2,143,895 + Shew_1865 0.40 +0.9 2,144,053 - Shew_1865 0.49 +0.8 2,144,293 + Shew_1865 0.63 +1.0 2,144,483 - Shew_1865 0.74 +0.9 2,145,145 + Shew_1866 0.81 +0.4 2,145,418 + Shew_1867 0.49 +1.6 2,145,546 + Shew_1867 0.82 +1.1 2,145,685 + +0.3 2,145,874 + +0.8
Or see this region's nucleotide sequence