Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1475 and Shew_1476 are separated by 57 nucleotides Shew_1476 and Shew_1477 overlap by 8 nucleotides Shew_1477 and Shew_1478 are separated by 49 nucleotides Shew_1478 and Shew_1479 overlap by 4 nucleotides
Shew_1475: Shew_1475 - hypothetical protein (RefSeq), at 1,700,959 to 1,701,648
_1475
Shew_1476: Shew_1476 - RNA polymerase sigma factor (RefSeq), at 1,701,706 to 1,702,344
_1476
Shew_1477: Shew_1477 - anti-ECFsigma factor, ChrR (RefSeq), at 1,702,337 to 1,703,086
_1477
Shew_1478: Shew_1478 - hypothetical protein (RefSeq), at 1,703,136 to 1,704,080
_1478
Shew_1479: Shew_1479 - deoxyribodipyrimidine photo-lyase (RefSeq), at 1,704,077 to 1,705,513
_1479
Position (kb)
1702
1703
1704 Strain fitness (log2 ratio)
-1
0
1 at 1701.413 kb on + strand, within Shew_1475 at 1701.744 kb on - strand at 1701.933 kb on - strand, within Shew_1476 at 1702.007 kb on - strand, within Shew_1476 at 1702.203 kb on + strand, within Shew_1476 at 1702.743 kb on + strand, within Shew_1477 at 1702.941 kb on - strand, within Shew_1477 at 1703.045 kb on - strand at 1703.331 kb on + strand, within Shew_1478 at 1703.345 kb on - strand, within Shew_1478
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 1,701,413 + Shew_1475 0.66 -0.9 1,701,744 - +1.7 1,701,933 - Shew_1476 0.36 +0.7 1,702,007 - Shew_1476 0.47 +0.3 1,702,203 + Shew_1476 0.78 +0.5 1,702,743 + Shew_1477 0.54 +0.7 1,702,941 - Shew_1477 0.81 +1.0 1,703,045 - +0.2 1,703,331 + Shew_1478 0.21 +0.0 1,703,345 - Shew_1478 0.22 +0.2
Or see this region's nucleotide sequence