Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1221 and Shew_1222 are separated by 363 nucleotides Shew_1222 and Shew_1223 are separated by 29 nucleotides Shew_1223 and Shew_1224 are separated by 132 nucleotides Shew_1224 and Shew_1225 are separated by 277 nucleotides Shew_1225 and Shew_1226 are separated by 85 nucleotides
Shew_1221: Shew_1221 - sodium ion-translocating decarboxylase, beta subunit (RefSeq), at 1,419,368 to 1,420,501
_1221
Shew_1222: Shew_1222 - hypothetical protein (RefSeq), at 1,420,865 to 1,421,050
_1222
Shew_1223: Shew_1223 - hypothetical protein (RefSeq), at 1,421,080 to 1,421,265
_1223
Shew_1224: Shew_1224 - sodium:dicarboxylate symporter (RefSeq), at 1,421,398 to 1,422,648
_1224
Shew_1225: Shew_1225 - hypothetical protein (RefSeq), at 1,422,926 to 1,423,348
_1225
Shew_1226: Shew_1226 - peptidase M16 domain-containing protein (RefSeq), at 1,423,434 to 1,426,268
_1226
Position (kb) 1421
1422
1423 Strain fitness (log2 ratio) -1
0
1
2 at 1420.573 kb on + strand at 1420.581 kb on - strand at 1420.690 kb on - strand at 1420.975 kb on - strand, within Shew_1222 at 1421.139 kb on + strand, within Shew_1223 at 1421.575 kb on + strand, within Shew_1224 at 1421.705 kb on + strand, within Shew_1224 at 1421.713 kb on - strand, within Shew_1224 at 1422.135 kb on + strand, within Shew_1224 at 1422.312 kb on + strand, within Shew_1224 at 1422.312 kb on + strand, within Shew_1224 at 1422.320 kb on - strand, within Shew_1224 at 1422.720 kb on + strand at 1423.070 kb on + strand, within Shew_1225 at 1423.089 kb on + strand, within Shew_1225 at 1423.251 kb on + strand, within Shew_1225 at 1423.400 kb on + strand at 1423.400 kb on + strand at 1423.408 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 1,420,573 + +0.1 1,420,581 - -0.0 1,420,690 - -0.0 1,420,975 - Shew_1222 0.59 +0.0 1,421,139 + Shew_1223 0.32 -0.4 1,421,575 + Shew_1224 0.14 +1.9 1,421,705 + Shew_1224 0.25 +1.8 1,421,713 - Shew_1224 0.25 +1.7 1,422,135 + Shew_1224 0.59 +2.0 1,422,312 + Shew_1224 0.73 +1.9 1,422,312 + Shew_1224 0.73 +2.3 1,422,320 - Shew_1224 0.74 +1.8 1,422,720 + +1.7 1,423,070 + Shew_1225 0.34 +0.6 1,423,089 + Shew_1225 0.39 +0.4 1,423,251 + Shew_1225 0.77 +0.3 1,423,400 + -0.4 1,423,400 + +0.8 1,423,408 - +0.1
Or see this region's nucleotide sequence