Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1120 and Shew_1121 are separated by 53 nucleotides Shew_1121 and Shew_1122 are separated by 76 nucleotides Shew_1122 and Shew_1123 are separated by 2 nucleotides Shew_1123 and trmB are separated by 235 nucleotides trmB and Shew_1125 are separated by 30 nucleotides
Shew_1120: Shew_1120 - cytochrome B561 (RefSeq), at 1,296,554 to 1,297,108
_1120
Shew_1121: Shew_1121 - hypothetical protein (RefSeq), at 1,297,162 to 1,297,695
_1121
Shew_1122: Shew_1122 - hypothetical protein (RefSeq), at 1,297,772 to 1,298,050
_1122
Shew_1123: Shew_1123 - A/G-specific adenine glycosylase (RefSeq), at 1,298,053 to 1,299,159
_1123
Shew_1124: trmB - tRNA (guanine-N(7))-methyltransferase (RefSeq), at 1,299,395 to 1,300,111
trmB
Shew_1125: Shew_1125 - hypothetical protein (RefSeq), at 1,300,142 to 1,300,465
_1125
Position (kb) 1298
1299
1300 Strain fitness (log2 ratio) -1
0
1 at 1297.170 kb on + strand at 1297.249 kb on + strand, within Shew_1121 at 1297.406 kb on + strand, within Shew_1121 at 1297.436 kb on + strand, within Shew_1121 at 1297.814 kb on - strand, within Shew_1122 at 1297.983 kb on + strand, within Shew_1122 at 1298.279 kb on - strand, within Shew_1123 at 1298.533 kb on + strand, within Shew_1123 at 1298.781 kb on - strand, within Shew_1123 at 1298.920 kb on + strand, within Shew_1123 at 1298.943 kb on + strand, within Shew_1123 at 1298.944 kb on + strand, within Shew_1123 at 1299.009 kb on + strand, within Shew_1123 at 1299.419 kb on - strand at 1299.464 kb on - strand at 1299.740 kb on - strand, within trmB at 1299.801 kb on + strand, within trmB
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 1,297,170 + +0.5 1,297,249 + Shew_1121 0.16 +0.2 1,297,406 + Shew_1121 0.46 +0.1 1,297,436 + Shew_1121 0.51 +0.5 1,297,814 - Shew_1122 0.15 +1.1 1,297,983 + Shew_1122 0.76 +0.7 1,298,279 - Shew_1123 0.20 +1.7 1,298,533 + Shew_1123 0.43 +0.4 1,298,781 - Shew_1123 0.66 +0.6 1,298,920 + Shew_1123 0.78 +0.6 1,298,943 + Shew_1123 0.80 +0.6 1,298,944 + Shew_1123 0.80 +0.8 1,299,009 + Shew_1123 0.86 +0.4 1,299,419 - -0.2 1,299,464 - -0.1 1,299,740 - trmB Shew_1124 0.48 -1.1 1,299,801 + trmB Shew_1124 0.57 +0.4
Or see this region's nucleotide sequence