Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0739 and Shew_0740 are separated by 56 nucleotides Shew_0740 and Shew_0741 are separated by 86 nucleotides Shew_0741 and Shew_0742 are separated by 141 nucleotides
Shew_0739: Shew_0739 - aromatic amino acid permease (RefSeq), at 840,729 to 842,000
_0739
Shew_0740: Shew_0740 - L-serine dehydratase 1 (RefSeq), at 842,057 to 843,424
_0740
Shew_0741: Shew_0741 - GCN5-related N-acetyltransferase (RefSeq), at 843,511 to 843,954
_0741
Shew_0742: Shew_0742 - hypothetical protein (RefSeq), at 844,096 to 844,401
_0742
Position (kb) 842
843
844 Strain fitness (log2 ratio) -2
-1
0
1 at 841.065 kb on - strand, within Shew_0739 at 841.078 kb on - strand, within Shew_0739 at 841.519 kb on - strand, within Shew_0739 at 841.531 kb on + strand, within Shew_0739 at 841.565 kb on - strand, within Shew_0739 at 841.584 kb on - strand, within Shew_0739 at 841.871 kb on - strand, within Shew_0739 at 842.059 kb on + strand at 842.087 kb on + strand at 842.096 kb on - strand at 842.363 kb on - strand, within Shew_0740 at 842.557 kb on - strand, within Shew_0740 at 842.864 kb on - strand, within Shew_0740 at 842.984 kb on - strand, within Shew_0740 at 843.375 kb on + strand at 843.716 kb on + strand, within Shew_0741 at 843.846 kb on + strand, within Shew_0741 at 843.874 kb on - strand, within Shew_0741 at 843.998 kb on - strand at 844.056 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 841,065 - Shew_0739 0.26 +0.0 841,078 - Shew_0739 0.27 -0.3 841,519 - Shew_0739 0.62 +0.3 841,531 + Shew_0739 0.63 +0.9 841,565 - Shew_0739 0.66 +0.3 841,584 - Shew_0739 0.67 -0.6 841,871 - Shew_0739 0.90 +0.1 842,059 + +0.2 842,087 + -0.0 842,096 - +0.2 842,363 - Shew_0740 0.22 -0.2 842,557 - Shew_0740 0.37 +0.1 842,864 - Shew_0740 0.59 -1.8 842,984 - Shew_0740 0.68 +0.2 843,375 + -0.4 843,716 + Shew_0741 0.46 +0.3 843,846 + Shew_0741 0.75 -0.4 843,874 - Shew_0741 0.82 -0.1 843,998 - +0.2 844,056 - +0.7
Or see this region's nucleotide sequence