Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0613 and Shew_0614 overlap by 1 nucleotides Shew_0614 and Shew_0615 are separated by 154 nucleotides
Shew_0613: Shew_0613 - cysteine/glutathione ABC transporter membrane/ATP-binding component (RefSeq), at 693,986 to 695,749
_0613
Shew_0614: Shew_0614 - cysteine/glutathione ABC transporter membrane/ATP-binding component (RefSeq), at 695,749 to 697,524
_0614
Shew_0615: Shew_0615 - bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase (RefSeq), at 697,679 to 700,858
_0615
Position (kb) 695
696
697
698 Strain fitness (log2 ratio) -1
0
1 at 694.836 kb on + strand, within Shew_0613 at 694.927 kb on + strand, within Shew_0613 at 695.078 kb on + strand, within Shew_0613 at 695.222 kb on + strand, within Shew_0613 at 695.232 kb on + strand, within Shew_0613 at 695.487 kb on - strand, within Shew_0613 at 695.778 kb on + strand at 695.848 kb on + strand at 696.052 kb on - strand, within Shew_0614 at 696.774 kb on - strand, within Shew_0614 at 697.106 kb on - strand, within Shew_0614 at 697.141 kb on + strand, within Shew_0614 at 697.151 kb on - strand, within Shew_0614 at 697.229 kb on - strand, within Shew_0614 at 697.365 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 694,836 + Shew_0613 0.48 +1.3 694,927 + Shew_0613 0.53 -1.3 695,078 + Shew_0613 0.62 +0.4 695,222 + Shew_0613 0.70 +0.3 695,232 + Shew_0613 0.71 +0.3 695,487 - Shew_0613 0.85 +0.5 695,778 + -0.2 695,848 + +1.6 696,052 - Shew_0614 0.17 +1.0 696,774 - Shew_0614 0.58 +1.4 697,106 - Shew_0614 0.76 +0.0 697,141 + Shew_0614 0.78 +1.0 697,151 - Shew_0614 0.79 +0.3 697,229 - Shew_0614 0.83 +0.2 697,365 + -0.2
Or see this region's nucleotide sequence