Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0438 and Shew_0439 are separated by 107 nucleotides Shew_0439 and Shew_0440 are separated by 8 nucleotides Shew_0440 and Shew_0441 are separated by 130 nucleotides
Shew_0438: Shew_0438 - GumN family protein (RefSeq), at 488,537 to 489,421
_0438
Shew_0439: Shew_0439 - CheD, stimulates methylation of MCP proteins (RefSeq), at 489,529 to 490,161
_0439
Shew_0440: Shew_0440 - methyltransferase type 12 (RefSeq), at 490,170 to 491,024
_0440
Shew_0441: Shew_0441 - dehydratase (RefSeq), at 491,155 to 491,994
_0441
Position (kb) 490
491
492 Strain fitness (log2 ratio) -1
0
1 at 489.658 kb on + strand, within Shew_0439 at 489.916 kb on - strand, within Shew_0439 at 490.044 kb on + strand, within Shew_0439 at 490.214 kb on - strand at 490.405 kb on - strand, within Shew_0440 at 490.551 kb on - strand, within Shew_0440 at 490.600 kb on - strand, within Shew_0440 at 490.607 kb on + strand, within Shew_0440 at 490.742 kb on + strand, within Shew_0440 at 490.742 kb on + strand, within Shew_0440 at 490.742 kb on + strand, within Shew_0440 at 491.118 kb on - strand at 491.166 kb on + strand at 491.231 kb on + strand at 491.246 kb on + strand, within Shew_0441 at 491.254 kb on - strand, within Shew_0441 at 491.254 kb on - strand, within Shew_0441 at 491.254 kb on - strand, within Shew_0441 at 491.254 kb on - strand, within Shew_0441 at 491.254 kb on - strand, within Shew_0441 at 491.292 kb on + strand, within Shew_0441 at 491.300 kb on - strand, within Shew_0441 at 491.478 kb on - strand, within Shew_0441 at 491.559 kb on + strand, within Shew_0441 at 491.566 kb on - strand, within Shew_0441 at 491.621 kb on + strand, within Shew_0441 at 491.660 kb on + strand, within Shew_0441 at 491.793 kb on + strand, within Shew_0441 at 491.843 kb on - strand, within Shew_0441 at 491.843 kb on - strand, within Shew_0441
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 489,658 + Shew_0439 0.20 +0.2 489,916 - Shew_0439 0.61 +0.1 490,044 + Shew_0439 0.81 -0.2 490,214 - -0.4 490,405 - Shew_0440 0.27 +0.3 490,551 - Shew_0440 0.45 -0.1 490,600 - Shew_0440 0.50 +0.1 490,607 + Shew_0440 0.51 +0.3 490,742 + Shew_0440 0.67 +0.4 490,742 + Shew_0440 0.67 +1.3 490,742 + Shew_0440 0.67 -0.7 491,118 - +0.0 491,166 + +0.3 491,231 + +0.2 491,246 + Shew_0441 0.11 -0.2 491,254 - Shew_0441 0.12 +1.4 491,254 - Shew_0441 0.12 +0.2 491,254 - Shew_0441 0.12 +0.2 491,254 - Shew_0441 0.12 +0.1 491,254 - Shew_0441 0.12 +0.3 491,292 + Shew_0441 0.16 +0.5 491,300 - Shew_0441 0.17 -0.6 491,478 - Shew_0441 0.38 -0.1 491,559 + Shew_0441 0.48 -0.2 491,566 - Shew_0441 0.49 +0.3 491,621 + Shew_0441 0.55 +0.4 491,660 + Shew_0441 0.60 +0.1 491,793 + Shew_0441 0.76 -0.0 491,843 - Shew_0441 0.82 -0.2 491,843 - Shew_0441 0.82 +1.7
Or see this region's nucleotide sequence