Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0420 and Shew_0421 are separated by 185 nucleotides Shew_0421 and Shew_0422 are separated by 66 nucleotides Shew_0422 and Shew_0423 are separated by 77 nucleotides
Shew_0420: Shew_0420 - major facilitator transporter (RefSeq), at 473,839 to 475,071
_0420
Shew_0421: Shew_0421 - phage shock protein C, PspC (RefSeq), at 475,257 to 475,505
_0421
Shew_0422: Shew_0422 - hypothetical protein (RefSeq), at 475,572 to 476,057
_0422
Shew_0423: Shew_0423 - MerR family transcriptional regulator (RefSeq), at 476,135 to 476,557
_0423
Position (kb) 475
476 Strain fitness (log2 ratio) -1
0
1
2
3 at 474.621 kb on + strand, within Shew_0420 at 474.643 kb on + strand, within Shew_0420 at 474.916 kb on + strand, within Shew_0420 at 474.939 kb on - strand, within Shew_0420 at 475.024 kb on - strand at 475.146 kb on + strand at 475.154 kb on - strand at 475.214 kb on - strand at 475.280 kb on + strand at 475.380 kb on - strand, within Shew_0421 at 475.415 kb on - strand, within Shew_0421 at 475.429 kb on + strand, within Shew_0421 at 475.573 kb on + strand at 475.644 kb on + strand, within Shew_0422 at 475.668 kb on + strand, within Shew_0422 at 475.766 kb on + strand, within Shew_0422 at 475.876 kb on + strand, within Shew_0422 at 475.876 kb on + strand, within Shew_0422 at 476.065 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 474,621 + Shew_0420 0.63 +0.1 474,643 + Shew_0420 0.65 -0.2 474,916 + Shew_0420 0.87 +0.4 474,939 - Shew_0420 0.89 +0.1 475,024 - -0.5 475,146 + -0.3 475,154 - +0.2 475,214 - +0.8 475,280 + +0.2 475,380 - Shew_0421 0.49 -0.4 475,415 - Shew_0421 0.63 -0.1 475,429 + Shew_0421 0.69 +3.6 475,573 + -0.1 475,644 + Shew_0422 0.15 -0.1 475,668 + Shew_0422 0.20 -0.6 475,766 + Shew_0422 0.40 -0.3 475,876 + Shew_0422 0.63 +0.1 475,876 + Shew_0422 0.63 +0.5 476,065 + +0.2
Or see this region's nucleotide sequence