Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0375 and Shew_0376 are separated by 594 nucleotides Shew_0376 and Shew_0377 are separated by 99 nucleotides Shew_0377 and argS are separated by 14 nucleotides
Shew_0375: Shew_0375 - ATP-dependent protease peptidase subunit (RefSeq), at 422,504 to 423,028
_0375
Shew_0376: Shew_0376 - curlin-associated protein (RefSeq), at 423,623 to 425,317
_0376
Shew_0377: Shew_0377 - sporulation domain-containing protein (RefSeq), at 425,417 to 425,995
_0377
Shew_0378: argS - arginyl-tRNA synthetase (RefSeq), at 426,010 to 427,755
argS
Position (kb) 423
424
425
426 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 422.748 kb on + strand, within Shew_0375 at 423.158 kb on + strand at 423.412 kb on - strand at 423.541 kb on + strand at 423.572 kb on - strand at 423.742 kb on + strand at 423.862 kb on + strand, within Shew_0376 at 424.506 kb on - strand, within Shew_0376 at 424.542 kb on - strand, within Shew_0376 at 424.575 kb on + strand, within Shew_0376 at 424.583 kb on - strand, within Shew_0376 at 424.685 kb on + strand, within Shew_0376 at 424.965 kb on + strand, within Shew_0376 at 425.140 kb on + strand, within Shew_0376 at 425.311 kb on + strand at 425.319 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 422,748 + Shew_0375 0.46 +0.8 423,158 + +1.0 423,412 - +0.2 423,541 + +0.0 423,572 - +0.5 423,742 + +0.1 423,862 + Shew_0376 0.14 -0.1 424,506 - Shew_0376 0.52 -3.4 424,542 - Shew_0376 0.54 +0.1 424,575 + Shew_0376 0.56 -1.1 424,583 - Shew_0376 0.57 -0.1 424,685 + Shew_0376 0.63 +0.3 424,965 + Shew_0376 0.79 -0.1 425,140 + Shew_0376 0.89 -0.0 425,311 + +0.1 425,319 - +0.3
Or see this region's nucleotide sequence