Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0085 and Shew_0086 are separated by 260 nucleotides Shew_0086 and moaA are separated by 315 nucleotides
Shew_0085: Shew_0085 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 97,414 to 99,330
_0085
Shew_0086: Shew_0086 - electron-transferring-flavoproteindehydrogenase (RefSeq), at 99,591 to 101,240
_0086
Shew_0087: moaA - molybdenum cofactor biosynthesis protein A (RefSeq), at 101,556 to 102,569
moaA
Position (kb) 99
100
101
102 Strain fitness (log2 ratio) -1
0
1
2 at 98.628 kb on + strand, within Shew_0085 at 98.834 kb on - strand, within Shew_0085 at 99.029 kb on + strand, within Shew_0085 at 99.037 kb on - strand, within Shew_0085 at 99.117 kb on + strand, within Shew_0085 at 99.135 kb on + strand, within Shew_0085 at 99.179 kb on + strand at 99.179 kb on - strand at 99.179 kb on + strand at 99.210 kb on + strand at 99.293 kb on - strand at 99.352 kb on - strand at 99.352 kb on - strand at 99.352 kb on - strand at 99.363 kb on + strand at 99.365 kb on + strand at 99.417 kb on + strand at 99.640 kb on + strand at 99.757 kb on + strand, within Shew_0086 at 99.813 kb on - strand, within Shew_0086 at 99.814 kb on - strand, within Shew_0086 at 99.830 kb on + strand, within Shew_0086 at 100.019 kb on - strand, within Shew_0086 at 100.115 kb on + strand, within Shew_0086 at 100.145 kb on - strand, within Shew_0086 at 100.209 kb on + strand, within Shew_0086 at 100.228 kb on + strand, within Shew_0086 at 100.245 kb on + strand, within Shew_0086 at 100.253 kb on - strand, within Shew_0086 at 100.294 kb on + strand, within Shew_0086 at 100.326 kb on - strand, within Shew_0086 at 100.326 kb on - strand, within Shew_0086 at 100.427 kb on - strand, within Shew_0086 at 100.546 kb on + strand, within Shew_0086 at 100.546 kb on + strand, within Shew_0086 at 100.729 kb on - strand, within Shew_0086 at 100.740 kb on - strand, within Shew_0086 at 100.846 kb on + strand, within Shew_0086 at 100.972 kb on - strand, within Shew_0086 at 101.020 kb on - strand, within Shew_0086 at 101.178 kb on + strand at 101.564 kb on + strand at 101.755 kb on + strand, within moaA at 101.864 kb on - strand, within moaA at 101.956 kb on + strand, within moaA at 101.956 kb on + strand, within moaA at 101.956 kb on + strand, within moaA at 101.964 kb on - strand, within moaA at 101.964 kb on - strand, within moaA at 102.046 kb on - strand, within moaA at 102.118 kb on + strand, within moaA at 102.126 kb on - strand, within moaA
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 98,628 + Shew_0085 0.63 +0.0 98,834 - Shew_0085 0.74 +0.0 99,029 + Shew_0085 0.84 -0.2 99,037 - Shew_0085 0.85 -0.0 99,117 + Shew_0085 0.89 +0.1 99,135 + Shew_0085 0.90 +1.7 99,179 + -0.1 99,179 - -0.2 99,179 + -0.8 99,210 + -0.1 99,293 - +0.3 99,352 - -0.0 99,352 - +0.5 99,352 - -0.8 99,363 + -0.1 99,365 + -0.1 99,417 + +0.1 99,640 + +0.3 99,757 + Shew_0086 0.10 +0.7 99,813 - Shew_0086 0.13 +1.2 99,814 - Shew_0086 0.14 +0.8 99,830 + Shew_0086 0.14 +1.1 100,019 - Shew_0086 0.26 +0.6 100,115 + Shew_0086 0.32 +0.9 100,145 - Shew_0086 0.34 +0.8 100,209 + Shew_0086 0.37 +1.6 100,228 + Shew_0086 0.39 +0.8 100,245 + Shew_0086 0.40 +0.6 100,253 - Shew_0086 0.40 +0.9 100,294 + Shew_0086 0.43 +1.8 100,326 - Shew_0086 0.45 +1.0 100,326 - Shew_0086 0.45 +0.9 100,427 - Shew_0086 0.51 +0.9 100,546 + Shew_0086 0.58 +1.4 100,546 + Shew_0086 0.58 +0.9 100,729 - Shew_0086 0.69 +1.8 100,740 - Shew_0086 0.70 +0.8 100,846 + Shew_0086 0.76 +0.8 100,972 - Shew_0086 0.84 +1.1 101,020 - Shew_0086 0.87 +0.8 101,178 + +0.4 101,564 + -0.2 101,755 + moaA Shew_0087 0.20 +0.2 101,864 - moaA Shew_0087 0.30 -0.1 101,956 + moaA Shew_0087 0.39 +0.1 101,956 + moaA Shew_0087 0.39 -0.6 101,956 + moaA Shew_0087 0.39 -0.2 101,964 - moaA Shew_0087 0.40 +0.1 101,964 - moaA Shew_0087 0.40 +0.7 102,046 - moaA Shew_0087 0.48 -0.5 102,118 + moaA Shew_0087 0.55 -0.4 102,126 - moaA Shew_0087 0.56 -0.1
Or see this region's nucleotide sequence