Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_647 and Pf6N2E2_648 are separated by 47 nucleotides Pf6N2E2_648 and Pf6N2E2_649 overlap by 4 nucleotides Pf6N2E2_649 and Pf6N2E2_650 are separated by 16 nucleotides Pf6N2E2_650 and Pf6N2E2_651 overlap by 4 nucleotides
Pf6N2E2_647 - General secretion pathway protein G, at 736,222 to 736,671
_647
Pf6N2E2_648 - General secretion pathway protein K, at 736,719 to 737,666
_648
Pf6N2E2_649 - General secretion pathway protein L, at 737,663 to 738,835
_649
Pf6N2E2_650 - General secretion pathway protein M, at 738,852 to 739,400
_650
Pf6N2E2_651 - General secretion pathway protein D, at 739,397 to 741,760
_651
Position (kb) 737
738
739 Strain fitness (log2 ratio) -2
-1
0
1 at 736.670 kb on - strand at 736.670 kb on - strand at 737.400 kb on + strand, within Pf6N2E2_648 at 737.468 kb on + strand, within Pf6N2E2_648 at 737.618 kb on + strand at 737.662 kb on + strand at 738.077 kb on + strand, within Pf6N2E2_649 at 738.113 kb on - strand, within Pf6N2E2_649 at 738.413 kb on + strand, within Pf6N2E2_649 at 738.834 kb on - strand at 738.834 kb on - strand at 739.008 kb on + strand, within Pf6N2E2_650 at 739.152 kb on + strand, within Pf6N2E2_650 at 739.152 kb on + strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.156 kb on + strand, within Pf6N2E2_650 at 739.157 kb on - strand, within Pf6N2E2_650 at 739.597 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 736,670 - +0.5 736,670 - -1.9 737,400 + Pf6N2E2_648 0.72 -0.0 737,468 + Pf6N2E2_648 0.79 +0.3 737,618 + -1.7 737,662 + +0.1 738,077 + Pf6N2E2_649 0.35 -1.3 738,113 - Pf6N2E2_649 0.38 -2.2 738,413 + Pf6N2E2_649 0.64 -1.0 738,834 - +1.0 738,834 - +1.4 739,008 + Pf6N2E2_650 0.28 -0.5 739,152 + Pf6N2E2_650 0.55 +0.6 739,152 + Pf6N2E2_650 0.55 -0.2 739,153 - Pf6N2E2_650 0.55 +0.1 739,153 - Pf6N2E2_650 0.55 +0.6 739,153 - Pf6N2E2_650 0.55 +0.2 739,156 + Pf6N2E2_650 0.55 -0.0 739,157 - Pf6N2E2_650 0.56 +0.7 739,597 + +0.5
Or see this region's nucleotide sequence