Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_601 and Pf6N2E2_602 are separated by 45 nucleotides Pf6N2E2_602 and Pf6N2E2_603 overlap by 4 nucleotides Pf6N2E2_603 and Pf6N2E2_604 are separated by 13 nucleotides
Pf6N2E2_601 - GlpG protein (membrane protein of glp regulon), at 676,097 to 676,969
_601
Pf6N2E2_602 - FIG00954117: hypothetical protein, at 677,015 to 677,275
_602
Pf6N2E2_603 - FIG00953934: hypothetical protein, at 677,272 to 678,102
_603
Pf6N2E2_604 - Membrane alanine aminopeptidase N (EC 3.4.11.2), at 678,116 to 680,773
_604
Position (kb) 677
678
679 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 676.475 kb on + strand, within Pf6N2E2_601 at 676.475 kb on + strand, within Pf6N2E2_601 at 676.476 kb on - strand, within Pf6N2E2_601 at 676.476 kb on - strand, within Pf6N2E2_601 at 676.760 kb on + strand, within Pf6N2E2_601 at 676.787 kb on + strand, within Pf6N2E2_601 at 676.954 kb on + strand at 677.060 kb on + strand, within Pf6N2E2_602 at 677.199 kb on - strand, within Pf6N2E2_602 at 677.199 kb on - strand, within Pf6N2E2_602 at 677.338 kb on + strand at 677.339 kb on - strand at 677.408 kb on - strand, within Pf6N2E2_603 at 677.606 kb on - strand, within Pf6N2E2_603 at 677.606 kb on - strand, within Pf6N2E2_603 at 677.606 kb on - strand, within Pf6N2E2_603 at 677.606 kb on - strand, within Pf6N2E2_603 at 678.031 kb on + strand at 678.032 kb on - strand at 678.061 kb on - strand at 678.067 kb on - strand at 678.070 kb on + strand at 678.070 kb on + strand at 678.070 kb on + strand at 678.071 kb on - strand at 678.071 kb on - strand at 678.071 kb on - strand at 678.080 kb on - strand at 678.100 kb on + strand at 678.101 kb on - strand at 678.101 kb on - strand at 678.101 kb on - strand at 678.101 kb on - strand at 678.143 kb on + strand at 678.155 kb on + strand at 678.170 kb on + strand at 678.171 kb on - strand at 678.353 kb on + strand at 678.354 kb on - strand at 678.462 kb on - strand, within Pf6N2E2_604 at 678.462 kb on - strand, within Pf6N2E2_604 at 678.521 kb on + strand, within Pf6N2E2_604 at 678.521 kb on + strand, within Pf6N2E2_604 at 678.522 kb on - strand, within Pf6N2E2_604 at 678.525 kb on - strand, within Pf6N2E2_604 at 678.525 kb on - strand, within Pf6N2E2_604 at 678.686 kb on + strand, within Pf6N2E2_604 at 678.686 kb on + strand, within Pf6N2E2_604 at 678.687 kb on - strand, within Pf6N2E2_604 at 678.687 kb on - strand, within Pf6N2E2_604 at 678.773 kb on + strand, within Pf6N2E2_604 at 678.773 kb on + strand, within Pf6N2E2_604 at 678.773 kb on + strand, within Pf6N2E2_604 at 678.774 kb on - strand, within Pf6N2E2_604 at 678.774 kb on - strand, within Pf6N2E2_604 at 678.852 kb on + strand, within Pf6N2E2_604 at 678.853 kb on - strand, within Pf6N2E2_604 at 678.854 kb on + strand, within Pf6N2E2_604 at 678.855 kb on - strand, within Pf6N2E2_604 at 678.855 kb on - strand, within Pf6N2E2_604 at 678.866 kb on + strand, within Pf6N2E2_604 at 678.884 kb on - strand, within Pf6N2E2_604 at 678.884 kb on - strand, within Pf6N2E2_604 at 678.884 kb on - strand at 678.940 kb on + strand, within Pf6N2E2_604 at 678.941 kb on - strand, within Pf6N2E2_604 at 678.941 kb on - strand, within Pf6N2E2_604 at 679.028 kb on + strand, within Pf6N2E2_604 at 679.028 kb on + strand, within Pf6N2E2_604
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 676,475 + Pf6N2E2_601 0.43 +1.8 676,475 + Pf6N2E2_601 0.43 -0.4 676,476 - Pf6N2E2_601 0.43 +0.7 676,476 - Pf6N2E2_601 0.43 -1.2 676,760 + Pf6N2E2_601 0.76 -0.9 676,787 + Pf6N2E2_601 0.79 +1.1 676,954 + +0.4 677,060 + Pf6N2E2_602 0.17 +1.0 677,199 - Pf6N2E2_602 0.70 +1.1 677,199 - Pf6N2E2_602 0.70 -1.1 677,338 + +0.2 677,339 - -2.2 677,408 - Pf6N2E2_603 0.16 -0.9 677,606 - Pf6N2E2_603 0.40 -0.4 677,606 - Pf6N2E2_603 0.40 -1.2 677,606 - Pf6N2E2_603 0.40 -0.9 677,606 - Pf6N2E2_603 0.40 -1.2 678,031 + +1.6 678,032 - -0.4 678,061 - +0.3 678,067 - +0.3 678,070 + -3.2 678,070 + +0.0 678,070 + -2.5 678,071 - +0.3 678,071 - +0.2 678,071 - +0.6 678,080 - -2.6 678,100 + -1.3 678,101 - +0.5 678,101 - -0.9 678,101 - -1.4 678,101 - -0.4 678,143 + +0.8 678,155 + +0.6 678,170 + -1.2 678,171 - -1.2 678,353 + -0.7 678,354 - -1.8 678,462 - Pf6N2E2_604 0.13 -1.0 678,462 - Pf6N2E2_604 0.13 +0.1 678,521 + Pf6N2E2_604 0.15 +0.0 678,521 + Pf6N2E2_604 0.15 -0.6 678,522 - Pf6N2E2_604 0.15 -2.6 678,525 - Pf6N2E2_604 0.15 +0.8 678,525 - Pf6N2E2_604 0.15 +0.3 678,686 + Pf6N2E2_604 0.21 -1.6 678,686 + Pf6N2E2_604 0.21 +0.7 678,687 - Pf6N2E2_604 0.21 -1.0 678,687 - Pf6N2E2_604 0.21 -1.6 678,773 + Pf6N2E2_604 0.25 -0.3 678,773 + Pf6N2E2_604 0.25 +0.1 678,773 + Pf6N2E2_604 0.25 -0.9 678,774 - Pf6N2E2_604 0.25 -1.1 678,774 - Pf6N2E2_604 0.25 -0.9 678,852 + Pf6N2E2_604 0.28 -3.4 678,853 - Pf6N2E2_604 0.28 +0.6 678,854 + Pf6N2E2_604 0.28 -0.1 678,855 - Pf6N2E2_604 0.28 -0.7 678,855 - Pf6N2E2_604 0.28 -2.2 678,866 + Pf6N2E2_604 0.28 -0.2 678,884 - Pf6N2E2_604 0.29 -2.1 678,884 - Pf6N2E2_604 0.29 -1.0 678,884 - +0.6 678,940 + Pf6N2E2_604 0.31 -3.2 678,941 - Pf6N2E2_604 0.31 -1.1 678,941 - Pf6N2E2_604 0.31 -0.5 679,028 + Pf6N2E2_604 0.34 +0.7 679,028 + Pf6N2E2_604 0.34 -1.0
Or see this region's nucleotide sequence